Abstract
Plant cells transformed into octopine-synthesizing tumour cells by the bacterium Agrobacterium tumefaciens survive when cultured in the presence of homo-arginine (HA), whereas both normal plant cells and nopaline producing plant tumour cells do not. Survival of octopine crown gall cells is due to the activity of the enzyme lysopinedehydrogenase (LpDH) in these cells, which converts toxic homo-arginine into non-toxic homo-octopine. The selective toxicity of homo-arginine for normal cells can be applied for the enrichment of octopine Ti plasmid transformed plant cells vs normal plant cells in mixed cultures.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cattoir A, Degryse E, Jacobs M and Negrutiu I (1980) Inhibition of Barley and Arabidopsis callus growth by lysine analogues. Plant Sci Lett 17, 327–332
Davey MR, Cocking EC, Freeman H, Pearce N and Tudor I (1980) Transformation of Petunia protoplasts by isolated Agrobacterium plasmids. Plant Sci Lett 18, 307–313.
Goldman A (1977) Octopine and nopaline dehydrogenases in crown gall tumors. Plant Sci Lett 10, 49–58.
Hack E and Kemp JD (1977) Purification and characterization of the crown gall specific enzyme, octopine synthetase. Biochem Biophys Res Comm 78, 785–791.
Koekman BP, Ooms G, Klapwijk PM and Schilperoort RA (1979) Genetic map of an octopine Ti plasmid. Plasmid 2, 347–357.
Lemmers M, de Beuckeleer M, Holsters M, Zambryski P, Depicker A, Hernalsteens JP, van Montagu M and Schell J (1980) Internal organization, boundaries and integration of Ti plasmid DNA in nopaline crown gall tumours. J Mol Biol 144, 353–376.
Linsmaier EM and Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 8, 100–127.
Maliga P, Breznovits A and Marton L (1973) Streptomycin resistant plants from callus of haploid tobacco. Nature, New Biology 244, 29–30.
Marton L, Wullems GJ, Molendijk L and Schilperoort RA (1979) In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens. Nature 277, 129–131.
Merlo DJ, Nutter RC, Montoya AL, Garfinkel DJ, Drummond MH, Chilton M-D, Gordon MP and Nester EW (1980) The boundaries and copy numbers of Ti plasmid T-DNA vary in crown gall tumors. Molec Gen Genet 177, 637–643.
Murashige T and Skoog F (1962) A rapid method for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15, 473–497.
Ooms G, Hooykaas PJJ, Moolenaar G and Schilperoort RA (1981) Crown gall plant tumors of different morphology, induced by Agrobacterium tumefaciens octopine Ti plasmid mutants. analysis of T-DNA functions. Gene 14, 33–50.
Otten LABM, Vreugdenhil D and Schilperoort RA (1977) Properties of D(+)-lysopine dehydrogenase from crown gall tumor tissue. Biochim Biophys Acta 485, 268–277.
Otten LABM and Schilperoort RA (1978) A rapid microscale method for the detection of lysopine and nopaline dehydrogenase activities. Biochim Biophys Acta 527, 497–500.
Otten LABM (1979) Lysopine dehydrogenase and nopaline dehydrogenase from crown gall tumour tissues. Thesis, University of Leiden, The Netherlands.
Petit A and Tempé J (1976) Etude du métabolisme de l'homo octopine et de l'un de ses analogues par l'Agrobacterium tumefaciens. CR Acad Sci Paris t 282, 69–71.
Schröder J, Schröder G, Huisman H, Schilperoort RA and Schell J (1981) The mRNA for lysopine dehydrogenase in plant tumor cells is complementary to a Ti plasmid fragment. FEBS letters 129, 166–168.
van Slogteren GMS, Planqué K and Lekkerkerk J (1980) Evaluation of parameters affecting the initiation of division of protoplasts of haploid and diploid Nicotiana sylvestris and Nicotiana tabacum. Plant Sci Lett 20, 35–45.
Thomashow MW, Nutter R, Postle K, Chilton M-D, Blattner FR, Powell A, Gordon MP and Nester EW (1980) Recombination between higher plant DNA and Ti plasmid of Agrobacterium tumefaciens. Proc Natl Acad Sci USA 77, 6448–6452.
Thomashow MF, Nutter R, Montoya AL, Gordon MP and Nester EW (1980) Integration and organization of Ti plasmid sequences in crown gall tumors. Cell 19, 729–739.
Wullems GJ, Molendijk L, Ooms G and Schilperoort RA (1981) Retention of tumor markers in F1 progeny plants from in vitro induced octopine and nopaline tumor tissues. Cell 24, 719–727.
Wullems GJ, Molendijk L, Ooms G and Schilperoort RA (1981) Differential expression of crown gall tumor markers in transformants obtained after in vitro transformation of Nicotiana tabacum derived cell wall regenerating protoplasts by Agrobacterium tumefaciens. Proc Natl Acad Sci USA 78, 4344–4348.
Yadav NS, Postle K, Saiki RK, Thomashow MF and Chilton M-D (1980) T DNA of a crown gall teratoma is covalently joined to host plant DNA. Nature 287, 458–461.
Yamada S and Itano HA (1966) Phenanthrenequinone as an analytical reagent for arginine and other monosubstituted guanidines. Biochim Biophys Acta 130, 538–540.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Van Slogteren, G.M.S., Hooykaas, P.J.J., Planqué, K. et al. The lysopinedehydrogenase gene used as a marker for the selection of octopine crown gall cells. Plant Mol Biol 1, 133–142 (1982). https://doi.org/10.1007/BF00024976
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00024976