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Plasmacytomas and Hybridomas

Development and Applications

  • Chapter
Monoclonal Antibodies

Abstract

Köhler and Milstein (1975) launched a new era in immunological research by showing that somatic cell hybridization could be used to generate a continuous “hybridoma” cell line producing a monoclonal antibody. The hybridoma technology has already fulfilled one of the major goals that immunologists have been trying to achieve for years: the routine production of large amounts of homogeneous antibody against a wide variety of antigens. Each of the subsequent chapters in this monograph will illustrate the usefulness of such reagents.

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References

  • Abbas, A. K., and Klaus, G. G. B., 1977, Inhibition of antibody production in plasmacytoma cells by antigen, Eur. J. Immunol. 7:667.

    Article  PubMed  CAS  Google Scholar 

  • Adetugbo, K., Milstein, C., and Secher, D. S., 1977, Molecular analysis of spontaneous somatic mutants, Nature 265:299.

    Article  PubMed  CAS  Google Scholar 

  • Baumal, R. and Scharff, M. D., 1973, Synthesis, assembly and secretion of mouse immunoglobulin, Transplant. Rev. 14:163.

    PubMed  CAS  Google Scholar 

  • Baumal, R., Bloom, B., and Scharff, M. D., 1971, Induction of long-term lymphocyte lines from delayed hypersensitive human donors using specific antigen plus Epstein-Barr virus, Nature [New Biol] 230:20.

    CAS  Google Scholar 

  • Bernard, O., Nobumichi, H., and Tonegawa, S., 1978, Sequences of mouse immunoglobulin light chain genes before and after somatic changes, Cell 15:1133.

    Article  PubMed  CAS  Google Scholar 

  • Bloom, B. R., Diamond, B., Muschel, R., Rosen, N., Schneck, J., Damiani, G., Rosen, O., and Scharff, M. D., 1978, Genetic approaches to the mechanism of macrophage functions, Fed. Proc. 37:2765.

    PubMed  CAS  Google Scholar 

  • Capra, J. D., Tung, A. S., and Nisonoff, A., 1975a, Structural studies on induced antibodies with defined idiotypic specificities. I. The heavy chains of anti-p-azophenylarsonate antibodies from A/J mice bearing a cross-reactive idiotype, J. Immunol. 114:1548.

    PubMed  CAS  Google Scholar 

  • Capra, J. D., Tung, A. S., and Nisonoff, A., 1975b, Structural studies on induced antibodies with defined idiotypic specificities, J. Immunol. 115:414.

    PubMed  CAS  Google Scholar 

  • Cebra, J. J., Koo, P. H., and Ray, A., 1974, Specificity of antibodies: Primary structural basis of hapten binding, Science 186:263.

    Article  PubMed  CAS  Google Scholar 

  • Coffino, P., Knowles, B., Nathenson, S., and Scharff, M. D., 1971, Suppression of immunoglobulin synthesis by cellular hybridization. Nature [New Biol] 231:87.

    Article  CAS  Google Scholar 

  • Coffino, P., Baumal, R., Laskov, R., and Scharff, M. D., 1972, Cloning of mouse myeloma cells and detection of rare variants, J. Cell Physiol. 79:429.

    Article  PubMed  CAS  Google Scholar 

  • Cohn, M., 1967, Natural history of the myeloma, Cold Spring Harbor Symp. Quant. Biol. 32:211.

    Article  CAS  Google Scholar 

  • Cold Spring Harbor Symposia on Quantitative Biology, 1967, Vol. 32, Antibodies.

    Google Scholar 

  • Cook, W. D., and Scharff, M. D., 1977, Antigen-binding mutants of mouse myeloma cells, Proc. Natl. Acad. Sci. USA 74:5687.

    Article  PubMed  CAS  Google Scholar 

  • Cotton, R. G. H., and Milstein, C., 1973, Fusion of two imrnunoglobulin-producing myeloma cells, Nature 244:42.

    Article  PubMed  CAS  Google Scholar 

  • Cotton, R. G. H., Secher, D. S., and Milstein, C., 1973, Somatic mutation and the origin of antibody diversity. Clonal variability of the immunoglobulin produced by MOPC 21 cells in culture, Eur. J. Immunol. 3:135.

    Article  CAS  Google Scholar 

  • Current Topics in Microbiology and Immunology, 1978, Vol. 81, Lymphocyte Hybridomas (F. Melchers, M. Potter, and N. Warner, eds.), Springer-Verlag, Berlin, Heidelberg, New York.

    Google Scholar 

  • Davidson, R. L., and Gerald, P. S., 1976, Improved techniques for induction of mammalian cell hybridization by polyethylene glycol, Somat. Cell Genet. 2:165.

    Article  PubMed  CAS  Google Scholar 

  • Diamond, B., Bloom, B. R., and Scharff, M. D., 1978, The Fc receptors of primary and cultured phagocytic cells studied with homogeneous antibodies, J. Immunol 121:1329.

    PubMed  CAS  Google Scholar 

  • Francus, T., and Birshtein, B. K., 1978, An IgG2a-producing variant of an IgG2b-producing mouse myeloma cell line. Structural studies on the Fc region of parent and variant heavy chains, Biochemistry 17:4324.

    Article  PubMed  CAS  Google Scholar 

  • Fu, S. M., Chiorazzi, N., Kunkel, H. G., Halper, J. P., and Harris, S. R., 1978, Induction of in vitro differentiation and immunoglobulin synthesis of human leukemic B lymphocytes, J. Exp. Med. 148:1570.

    Article  PubMed  CAS  Google Scholar 

  • Galfre, G., Howe, S. C., Milstein, C., Butcher, G. W., and Howard, J. C., 1977, Antibodies to major histocompatibility antigens produced by hybrid cell lines, Nature 266:550.

    Article  PubMed  CAS  Google Scholar 

  • Galfre, G., Milstein, C., and Wright, B., 1979, R × rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG, Nature 277:131.

    Article  PubMed  CAS  Google Scholar 

  • Gefter, M. L., Margulies, D. H., and Scharff, M. D., 1977, A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells, Somat. Cell Genet. 2:231.

    Article  Google Scholar 

  • Haas, W., and von Boehmer, H., 1978, Techniques for separation and selection of antigen specific lymphocytes, Curr. Top. Microbiol Immunol 84.

    Google Scholar 

  • Haber, E., 1970, Antibodies of restricted heterogeneity for structural study, Fed. Proc. 29:66.

    PubMed  CAS  Google Scholar 

  • Haber, E., Margolies, M. N., and Cannon, L. E., 1977, Structure of the framework and complementarity regions of elicited antibodies, in: Antibodies in Human Diagnosis and Therapy (E. Haber and R. M. Krause, eds.), Raven Press, New York, pp. 45–78.

    Google Scholar 

  • Helmreich, E., Kern, M., and Eisen, H. N., 1961, The secretion of antibody by isolated lymph node cells, J. Biol. Chem. 236:464.

    PubMed  CAS  Google Scholar 

  • Horibata, K., and Harris, A. W., 1970, Mouse myelomas and lymphomas in culture, Exp. Cell Res. 60:61.

    Article  PubMed  CAS  Google Scholar 

  • Howard, J. C., Butcher, G. W., Galfre, G., and Milstein, C., 1978, Monoclonal anti-rat MHC (H-l) alloantibodies, Curr. Top. Microbiol. Immunol. 81:54.

    PubMed  CAS  Google Scholar 

  • Kearney, J. F., Radbruch, A., Liesegang, B., and Rajewsky, K., 1980, A new mouse myeloma cell line which has lost immunoglobulin expression but permits the construction of antibody secreting hybrid cells lines, J. Immunol. 123:1548.

    Google Scholar 

  • Kishimoto, T., Hirano, T., Kuritani, T., Yamamura, Y., Ralph, P., and Good, R. A., 1978, Induction of IgG production in human B lymphoblastoid cell lines with normal human T cells, Nature 271:756.

    Article  PubMed  CAS  Google Scholar 

  • Köhler, G., and Milstein, C., 1975, Continuous cultures of fused cells secreting antibody of predefined specificity, Nature 256:495.

    Article  PubMed  Google Scholar 

  • Köhler, G., and Milstein, C., 1976, Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion, Eur. J. Immunol. 6:511.

    Article  PubMed  Google Scholar 

  • Köhler, G., Howe, S. C., and Milstein, C., 1976, Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines, Eur. J. Immunol. 6:292.

    Article  PubMed  Google Scholar 

  • Krause, R. M., 1970, The search for antibodies with molecular uniformity, Adv. Immunol. 12:1.

    Article  PubMed  CAS  Google Scholar 

  • Kuehl, W. M., 1977, Synthesis of immunoglobulin in myeloma cells, Curr. Top. Microbiol. Immunol. 76:1.

    Article  PubMed  CAS  Google Scholar 

  • Laskov, R., and Scharff, M. D., 1970, Synthesis, assembly and secretion of gamma globulin by mouse myeloma cells, J. Exp. Med. 131:515.

    Article  PubMed  CAS  Google Scholar 

  • Littlefield, J. W., 1964, Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants, Science 145:709.

    Article  PubMed  CAS  Google Scholar 

  • Margulies, D. H., Kuehl, W. M., and Scharff, M. D., 1976, Somatic cell hybridization of mouse myeloma cells, Cell 8:405.

    Article  PubMed  CAS  Google Scholar 

  • Margulies, D. H., Cieplinski, W., Dharmgrongartama, B., Gefter, M. L., Morrison, S. L., Kelly, T., and Scharff, M. D., 1977, Regulation of immunoglobulin expression in mouse myeloma cells, Cold Spring Harbor Symp. Quant. Biol. 41:781.

    Article  PubMed  Google Scholar 

  • Melchers, F., 1978, T cell hybrids: Shortcut or dead end?, Nature 271:9.

    Article  Google Scholar 

  • Milstein, C., and Köhler, G., 1977, Cell fusion and the derivation of cell lines producing specific antibody, in: Antibodies in Human Diagnosis and Therapy (E. Haber and R. M. Krause, eds.), Raven Press, New York, pp. 271–286.

    Google Scholar 

  • Milstein, C., Adetugbo, K., Cowan, N. J., Kohler, G., Secher, D. S., and Wilde, C. D., 1977, Somatic cell genetics of antibody-secreting cells: Studies of clonal diversification and analysis by cell fusion, Cold Spring Harbor Symp. Quant. Biol. 41:793.

    Article  PubMed  Google Scholar 

  • Milstein, C., Galfre, G., Secher, D. S., and Springer, T., 1979, Monoclonal antibodies and cell surface antigens, Cell Biol. Int. Rep. 3(1): 1.

    Article  PubMed  CAS  Google Scholar 

  • Neauport-Sautes, C., Rabourdin-Combe, C., and Fridman, W. H., 1979, T-cell hybrids bear Fc receptors and secrete suppressor immunoglobulin binding factor, Nature 277:656.

    Article  PubMed  CAS  Google Scholar 

  • Pettengill, G. S., and Sorensen, G. D., 1967, Murine myeloma cells in suspension culture, Exp. Cell Res. 47:608.

    Article  PubMed  CAS  Google Scholar 

  • Potter, M., 1972, Immunoglobulin-producing tumors and myeloma proteins of mice, Physiol. Rev. 52:631.

    PubMed  CAS  Google Scholar 

  • Potter, M., 1978, Antigen-binding myeloma proteins of mice, Adv. Immunol 25:141.

    Article  Google Scholar 

  • Potter M., Rudikoff, S., Radian, E. A., and Vrana, M., 1977, Covalent structure of the antigen binding site: Antigen-binding myeloma proteins of the BALB/c mouse, in: Antibodies in Human Diagnosis and Therapy (E. Haber and R. M. Krause, eds.), Raven Press, New York, pp. 9–28.

    Google Scholar 

  • Ralph, P., Richard, J., and Cohn, M., 1975, Reticulum cell sarcoma: An effector cell in antibody-dependent cell-mediated immunity, J. Immunol. 114:898.

    PubMed  CAS  Google Scholar 

  • Rohrer, J. W., Odermatt, B., and Lynch R. G., 1979, Immunoregulation of murine myeloma: Isologous immunization with M315 induces idiotype-specific T cells that suppress IgA secretion by MOP-315 cells in vivo, J. Immunol. 122:2011.

    PubMed  CAS  Google Scholar 

  • Sakano, H., Rogers, J. H., Huppi, K., Brack, C., Traunecker, A., Maki, R., Wall, R., and Tonegawa S., 1978, Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segments, Nature 277:627.

    Article  Google Scholar 

  • Scharff, M. D., 1974, The synthesis, assembly and secretion of immunoglobulin: A biochemical and genetic approach, Harvey Lect. 69:125.

    Google Scholar 

  • Scharff, M. D., and Laskov, R., 1970, Synthesis and assembly of immunoglobulin polypeptide chains, Prog. Allergy 14:37.

    PubMed  CAS  Google Scholar 

  • Schulman, M., Wilde, C. D., and Köhler, G., 1978, A better cell line for making hybridomas secreting specific antibodies, Nature 276:269.

    Article  Google Scholar 

  • Seidman, J. G., Leder, A., Nau, M., Norman, B., and Leder, P., 1978, Antibody diversity: The structure of cloned immunoglobulin gene suggests a mechanism for generating new sequences, Science 202:11.

    Article  PubMed  CAS  Google Scholar 

  • Sharon, J., Morrison, S. L., and Kabat, E. A., 1979, Detection of specific hybridoma clones by replica immunoadsorption of their secreted antibodies, Proc. Natl Acad. Sci. USA 76:1420.

    Article  PubMed  CAS  Google Scholar 

  • Slavin, S., and Strober, S., 1978, Spontaneous muring B-cell leukemia, Nature 272:624.

    Article  PubMed  CAS  Google Scholar 

  • Steinitz, M., Klein, G., Koskimies, S., and Makela, O., 1977, EB virus-induced B lymphocyte cell lines producing specific antibody, Nature 269:420.

    Article  PubMed  CAS  Google Scholar 

  • Strosberg, A. D., Collins, J. J., Black, P. H., Malamud, D., Wilbert, S., Block, K. J., and Haber, E., 1974, Transformation by simian virus 40 of spleen cells from a hyperimmune rabbit: Demonstration of production of specific antibody to the immunizing antigen, Proc. Natl. Acad. Sci. USA 71:263.

    Article  PubMed  CAS  Google Scholar 

  • Taniguchi, M., Saito, T., and Tada, T., 1979, Antigen-specific suppressive factor produced by a transplantable I-J bearing T-cell hybridoma, Nature 278:555.

    Article  PubMed  CAS  Google Scholar 

  • Taussig, M. J., and Holliman, A., 1979, Structure of an antigen-specific suppressor factor produced by a hybrid T-cell line, Nature 277:308.

    Article  PubMed  CAS  Google Scholar 

  • Williamson, A., 1971, Biosynthesis of antibodies, Nature 231:359.

    Article  PubMed  CAS  Google Scholar 

  • Zurawski, V. R. Jr., Haber, E., and Black, P. H., 1978, Production of antibody to tetanus toxoid by continuous human lymphoblastoid cell lines, Science 199:1439.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Cell Biology, Albert Einstein College of Medicine, 10461, Bronx, New York, USA

    Dale E. Yelton, David H. Margulies, Betty Diamond & Matthew D. Scharff

Authors
  1. Dale E. Yelton
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  2. David H. Margulies
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  3. Betty Diamond
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  4. Matthew D. Scharff
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Editor information

Editors and Affiliations

  1. University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA

    Roger H. Kennett  & Thomas J. McKearn  & 

  2. The Wistar Institute, Philadelphia, Pennsylvania, USA

    Kathleen B. Bechtol

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© 1980 Plenum Press, New York

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Yelton, D.E., Margulies, D.H., Diamond, B., Scharff, M.D. (1980). Plasmacytomas and Hybridomas. In: Kennett, R.H., McKearn, T.J., Bechtol, K.B. (eds) Monoclonal Antibodies. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7505-4_1

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  • DOI: https://doi.org/10.1007/978-1-4615-7505-4_1

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-7507-8

  • Online ISBN: 978-1-4615-7505-4

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