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Monoclonal Antibodies

Hybridomas: A New Dimension in Biological Analyses

  • Roger H. Kennett
  • Thomas J. McKearn
  • Kathleen B. Bechtol

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Dale E. Yelton, David H. Margulies, Betty Diamond, Matthew D. Scharff
      Pages 3-17
    3. Vincent R. Zurawski Jr., Paul H. Black, Edgar Haber
      Pages 19-33
  3. Analysis of Immunoglobulin Structure and Genetics

    1. Front Matter
      Pages 35-35
    2. Brian Clevinger, James Schilling, Rogers Griffith, Daniel Hansburg, Leroy Hood, Joseph Davie
      Pages 37-48
    3. Kathleen Denis, Roger H. Kennett, Norman Klinman, Christine Molinaro, Linda Sherman
      Pages 49-59
    4. Hans Hengartner, Tomaso Meo, Edith Müller
      Pages 61-71
  4. Detection and Analysis of Human Gene Products

    1. Front Matter
      Pages 73-73
    2. E. Solomon, E. A. Jones
      Pages 75-102
    3. C. A. Slaughter, M. C. Coseo, C. Abrams, M. P. Cancro, H. Harris
      Pages 103-120
    4. Richard A. Goldsby, Barbara A. Osborne, Edgar G. Engleman
      Pages 121-135
    5. Ronald Levy, Jeanette Dilley, Sherri Brown, Yehudit Bergman
      Pages 137-153
    6. Roger H. Kennett, Zdenka L. Jonak, Kathleen B. Bechtol
      Pages 155-168
  5. Monoclonal Antibodies as Probes in the Study of Cellular Differentiation and Immunogenetics

    1. Front Matter
      Pages 169-169
    2. Kathleen B. Bechtol, Zdenka L. Jonak, Roger H. Kennett
      Pages 171-184
    3. Timothy A. Springer
      Pages 185-217
    4. Thomas J. McKearn, Dawn E. Smilek, Frank W. Fitch
      Pages 219-234
    5. Jeffrey A. Ledbetter, James W. Goding, Takeshi Tokuhisa, Leonard A. Herzenberg
      Pages 235-249
    6. Donald W. Mason, Roger J. Brideau, W. Robert McMaster, Michael Webb, Robert A. H. White, Alan F. Williams
      Pages 251-273
    7. Irwin D. Bernstein, Robert C. Nowinski, Milton R. Tam, Brian McMaster, L. L. Houston, Edward A. Clark
      Pages 275-291
  6. Monoclonal Antibodies to Microorganisms

    1. Front Matter
      Pages 293-293
    2. Robert C. Nowinski, Mary R. Stone, Milton R. Tam, Mark E. Lostrom, W. Neal Burnette, Paul V. O’Donnell
      Pages 295-316
    3. Walter Gerhard, Jonathan Yewdell, Mark E. Frankel, A. Dwight Lopes, Louis Staudt
      Pages 317-333
    4. Hilary Koprowski, Tadeusz Wiktor
      Pages 335-351
  7. Back Matter
    Pages 361-423

About this book

Introduction

On August 7, 1975, Kohler and Milstein published in Nature (256:495) a report describing "Continuous cultures of fused cells secreting antibody of predefined specificity. " Their report has become a classic and has already had a profound effect on basic and applied research in biology and medicine. By the time the first Workshop on Lymphocyte Hybridomas (Current Topics in Microbiology and Im­ munology 81, 1978) was held on April 3-5, 1978, in Bethesda, Maryland, investi­ gators from many laboratories had made hybrids between plasmacytomas and spleen cells from immunized animals and had obtained monoclonal antibodies reacting with a broad variety of antigenic determinants. At the time Kohler and Milstein introduced this new technology, the editors of this volume were involved in the production of antisera against differentiation antigens (K. B. B. ), histocompatibility antigens (T. ]. McK. ), and human tumor­ associated antigens (R. H. K. ). Because of the potential usefulness of monoclonal antibodies in these areas, we each began production of hybridomas and analysis of the resulting monoclonal reagents. One of the most interesting aspects of participation in the early stages of the development and application of hybrid­ oma technology has been observing how the implications of the initial observa­ tions gradually spread first among the practitioners of immunology and immu­ nogenetics, and then to other areas of the biological sciences, such as developmental biology, biochemistry, human genetics, and cell and tumor biology.

Keywords

antibody antigen genetics histocompatibility immunology microbiology

Editors and affiliations

  • Roger H. Kennett
    • 1
  • Thomas J. McKearn
    • 1
  • Kathleen B. Bechtol
    • 2
  1. 1.University of Pennsylvania School of MedicinePhiladelphiaUSA
  2. 2.The Wistar InstitutePhiladelphiaUSA

Bibliographic information