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A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells

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Somatic Cell Genetics

Abstract

A simple method is described for promoting the fusion of mouse myeloma cells in suspension with polyethylene glycol (PEG 1000). By carefully controlling the concentration of PEG and the time of exposure of the cells, it was possible to obtain hybridization frequencies several-hundred-fold higher than those obtained with Sendai virus.

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Author notes

  1. Malcolm L. Gefter

    Present address: Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts

Authors and Affiliations

  1. Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York

    Malcolm L. Gefter, David H. Margulies & Matthew D. Scharff

Authors
  1. Malcolm L. Gefter
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  2. David H. Margulies
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  3. Matthew D. Scharff
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Gefter, M.L., Margulies, D.H. & Scharff, M.D. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells. Somat Cell Mol Genet 3, 231–236 (1977). https://doi.org/10.1007/BF01551818

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  • DOI: https://doi.org/10.1007/BF01551818

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