Abstract
Sheepgrass (Leymus chinensis (Trin.) Tzvel.) has different environmental adaptation and spans different soil and climatic conditions and supports soil and water protection, ecological construction, and animal husbandry, especially in the Northern China. It has excellent genetic resources and is worth digging. The development and application of next-generation sequencing technology (NGS) have been greatly accelerated gene resource mining in sheepgrass. The establishment of omics database of diverse stress treatments of freezing, saline-alkaline, drought, wounding, defoliation, and animal saliva deposition and organs at specific developmental stages has promoted the global overview of particular biological properties and provides resources to discover novel genes in sheepgrass. A number of stress-related genes has been cloned and experimentally validated, and the elite genes in sheepgrass may provide a choice for forage and crops improvement. In the future, the availability of sheepgrass genome and the development of new technologies, such as CRISPR/Cas9 genome editing, phenotyping and genotyping, genome-wide association study (GWAS), etc., will accelerate sheepgrass gene resource mining and utilities.
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References
Bai YF, Han XG, Wu JG et al (2004) Ecosystem stability and compensatory effects in the Inner Mongolia grassland. Nature 431:181–184
Bentley DR, Balasubramanian S, Swerdlow HP et al (2008) Accurate whole human genome sequencing using reversible terminator chemistry. Nature 456:53–59
Chen S, Li XQ, Zhao A et al (2009) Genes and pathways induced in early response to defoliation in rice seedlings. Curr Issues Mol Biol 11(2):81–100
Chen S, Huang X, Yan X et al (2013) Transcriptome analysis in sheepgrass (Leymus chinensis): a dominant perennial grass of the eurasian steppe. PLoS One 8:e67974
Chen S, Cai Y, Zhang L et al (2014) Transcriptome analysis reveals common and distinct mechanisms for sheepgrass (Leymus chinensis) responses to defoliation compared to mechanical wounding. PLoS One 9:e89495
Cheng L, Li X, Huang X et al (2013) Overexpression of sheepgrass R1-MYB transcription factor LcMYB1 confers salt tolerance in transgenic Arabidopsis. Plant Physiol Biochem 70:252–60
Eid J, Fehr A, Gray J et al (2009) Real-time DNA sequencing from single polymerase molecules. Science 323:133–138
Gao Q, Li XX, Jia JT et al (2016) Overexpression of a novel cold-responsive transcript factor LcFIN1 from Leymus chinensis enhances tolerance to low temperature stress in transgenic plants. Plant Biotechnol J 14:861–874
Haas BJ, Papanicolaou A, Yassour M et al (2013) De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nat Protoc 8(8):1494–1512
Huang X, Peng X, Zhang L et al (2014) Bovine serum albumin in saliva mediates grazing response in Leymus chinensis revealed by RNA sequencing. BMC Genomics 15:1126
Kopecký D, Studer B (2014) Emerging technologies advancing forage and turf grass genomics. Biotechnol Adv 32(1):190–199
Larson SR, Kishii M, Tsujimoto H et al (2012) Leymus EST linkage maps identify 4NsL-5NsL reciprocal translocation, wheat-Leymus chromosome introgressions, and functionally important gene loci. Theor Appl Genet 124:189–206
Li XX, Hou SL, Gao Q et al (2013a) LcSAIN1, a novel salt-induced gene from sheepgrass, confers salt stress tolerance in transgenic Arabidopsis and rice. Plant Cell Physiol 54(7):1172–1185
Li XX, Gao Q, Liang Y et al (2013b) A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis. Plant Physiol Biochem 64:52–59
Liu GS, Li XF (2011) Germplasm of sheepgrass. Science Presss, Beijing
Liu D, Hu R, Palla KJ, Tuskan GA, Yang X (2016) Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research. Curr Opin Plant Biol 30:70–77
Liu Z, Liu P, Qi D et al (2017) Enhancement of cold and salt tolerance of Arabidopsis by transgenic expression of the S-adenosylmethionine decarboxylase gene from Leymus chinensis. J Plant Physiol 211:90–99
Ma HY, Liang ZW (2007) Effects of different soil pH and soil extracts on the germination and seedling growth of Leymus chinensis. Chin Bull Bot 24:181–188
Ma T, Li ML, Zhao AG et al (2014) Journal of Plant Physiology. LcWRKY5: an unknown function gene from Leymus chinensis improves drought tolerance in transgenic Arabidopsis. Plant Cell Rep 33:1507–1518
Margulies M, Egholm M, Altman WE, Attiya S, Bader JS et al (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437:376–380
McKernan KJ, Peckham HE, Costa GL et al (2009) Sequence and structural variation in a human genome uncovered by short-read, massively parallel ligation sequencing using two-base encoding. Genome Res 19:1527–1541
Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681
Nevo E, Chen GX (2010) Drought and salt tolerances in wild relatives for wheat and barley improvement. Plant Cell Environ 33:670–685
Pandey MK, Roorkiwal M, Singh VK et al (2016) Emerging genomic tools for legume breeding: current status and future prospects. Front Plant Sci 7:455
Peng XJ, Ma XY, Fan WH et al (2011) Improved drought and salt tolerance of Arabidopsis thaliana by transgenic expression of a novel DREB gene from Leymus chinensis. Plant Cell Rep 30:1493–1502
Peng XJ, Zhang LX, Zhang LX et al (2013) The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis. Plant Cell Tissue Organ Cult 113:245–256
Ruttink T, Sterck L, Rohde A et al (2013) Orthology guided assembly in highly heterozygous crops: creating a reference transcriptome to uncover genetic diversity in Lolium perenne. Plant Biotechnol J 11:605–617
Su M, Li XX, Li XF et al (2013) Molecular characterization and defoliation-induced expression of a sucrose transporter LcSUT1 gene in sheep grass (Leymus chinensis). Plant Mol Biol Report 31:1184–1191
Sun Y, Wang F, Wang N et al (2013) Transcriptome exploration in Leymus chinensis under saline-alkaline treatment using 454 pyrosequencing. PLoS One 8(1):e53632
Thompson JF, Steinmann KE (2010) Single molecule sequencing with a HeliScope genetic analysis system. Curr Protoc Mol Biol. Chapter 7
Wang RZ, Chen L, Bai YG et al (2008) Seasonal dynamics in resource partitioning to growth and storage in response to drought in a perennial rhizomatous grass, Leymus chinensis. J Plant Growth Regul 27:39–48
Wang LJ, Li XF, Chen SY et al (2009) Enhanced drought tolerance in transgenic Leymus chinensis plants with constitutively expressed wheat TaLEA3. Biotechnol Lett 31:313–319
Zhao PC, Liu PP, Yuan GX et al (2016) New insights on drought stress response by global investigation of gene expression changes in Sheepgrass (Leymus chinensis). Front Plant Sci 7:954
Zhou QY, Jia JT, Huang X et al (2014) The large-scale investigation of gene expression in Leymus chinensis stigmas provides a valuable resource for understanding the mechanisms of poaceae self-incompatibility. BMC Genomics 15:399
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Chen, S., Huang, X., Yan, X., Zhang, L., Zhao, P. (2019). Advances on Gene Resource Mining in Sheepgrass (Leymus chinensis). In: Liu, G., Li, X., Zhang, Q. (eds) Sheepgrass (Leymus chinensis): An Environmentally Friendly Native Grass for Animals. Springer, Singapore. https://doi.org/10.1007/978-981-13-8633-6_11
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DOI: https://doi.org/10.1007/978-981-13-8633-6_11
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