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Guanylate Cyclase and Cyclic GMP

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Thomas Krieg

    1. Addenbrooke’s Hospital, Department of Medicine, University of Cambridge, Cambridge, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Robert Lukowski

    1. Toxicology and Clinical Pharmacy, Department of Pharmacology, Universität Tübingen, Tübingen, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Presents current up-to-date methodologies
  • Features tips on trouble shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1020)

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About this book

Recently, many ground-breaking steps have been made towards  better understanding NO/cGMP/PKG pathways, its components, substrates, and their localization within a given cell. These advances were only possible due to the development of sophisticated new techniques in the field. In Guanylate Cyclase and Cyclic GMP: Methods and Protocols expert researchers in the field seek to provide an overview of novel techniques to identify various elements of the NO/cGMP/PKG pathway and further characterize their function, signaling, localization, and importance on a cellular level and in whole animal models providing a higher patho-/physiological integration and relevance.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

 

Authoritative and practical, Guanylate Cyclase and Cyclic GMP: Methods and Protocols seeks to provide  scientist current methods and a useful guide towards the possibility to apply these techniques to their own research.

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
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  2. NO/cGMP: The Past, the Present, and the Future

    • Michael Russwurm, Corina Russwurm, Doris Koesling, Evanthia Mergia
    Pages 1-16

  3. cGMP-Dependent Protein Kinases (cGK)

    • Franz Hofmann, Jörg W. Wegener
    Pages 17-50

  4. Enzyme Assays for cGMP Hydrolyzing Phosphodiesterases

    • Sergei D. Rybalkin, Thomas R. Hinds, Joseph A. Beavo
    Pages 51-62

  5. Radioimmunoassay for the Quantification of cGMP Levels in Cells and Tissues

    • Ronald Jäger, Dieter Groneberg, Barbara Lies, Noomen Bettaga, Michaela Kümmel, Andreas Friebe
    Pages 63-72

  6. Hyperspectral Imaging of FRET-Based cGMP Probes

    • Thomas C. Rich, Andrea L. Britain, Tiffany Stedman, Silas J. Leavesley
    Pages 73-88

  7. Visualization of cGMP with cGi Biosensors

    • Martin Thunemann, Natalie Fomin, Christian Krawutschke, Michael Russwurm, Robert Feil
    Pages 89-120

  8. Advances and Techniques to Measure cGMP in Intact Cardiomyocytes

    • Konrad R. Götz, Viacheslav O. Nikolaev
    Pages 121-129

  9. Real-Time Monitoring the Spatiotemporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cells

    • Kara F. Held, Wolfgang R. Dostmann
    Pages 131-145

  10. Methods for Identification of cGKI Substrates

    • Katharina Salb, Jens Schlossmann
    Pages 147-162

  11. Approaches for Monitoring PKG1α Oxidative Activation

    • Joseph Robert Burgoyne, Philip Eaton
    Pages 163-173

  12. Analysis of cGMP Signaling in Adipocytes

    • Katja Jennissen, Bodo Haas, Michaela M. Mitschke, Franziska Siegel, Alexander Pfeifer
    Pages 175-192

  13. A Genetic Strategy for the Analysis of Individual Axon Morphologies in cGMP Signalling Mutant Mice

    • Hannes Schmidt, Gohar Ter-Avetisyan, Fritz G. Rathjen
    Pages 193-204

  14. Receptor Binding Assay for NO-Independent Activators of Soluble Guanylate Cyclase

    • Peter M. Schmidt, Johannes-Peter Stasch
    Pages 205-214

  15. Direct Intrathecal Drug Delivery in Mice for Detecting In Vivo Effects of cGMP on Pain Processing

    • Ruirui Lu, Achim Schmidtko
    Pages 215-221

  16. The Geisler Method: Tracing Activity-Dependent cGMP Plasticity Changes upon Double Detection of mRNA and Protein on Brain Slices

    • Wibke Singer, Hyun-Soon Geisler, Marlies Knipper
    Pages 223-233

  17. Detection of cGMP in the Degenerating Retina

    • Stylianos Michalakis, Jianhua Xu, Martin Biel, Xi-Qin Ding
    Pages 235-245

  18. Back Matter

    Pages 247-249
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Editors and Affiliations

  • Addenbrooke’s Hospital, Department of Medicine, University of Cambridge, Cambridge, United Kingdom

    Thomas Krieg

  • Toxicology and Clinical Pharmacy, Department of Pharmacology, Universität Tübingen, Tübingen, Germany

    Robert Lukowski

Bibliographic Information

  • Book Title: Guanylate Cyclase and Cyclic GMP

  • Book Subtitle: Methods and Protocols

  • Editors: Thomas Krieg, Robert Lukowski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-459-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-458-6Published: 25 May 2013

  • Softcover ISBN: 978-1-4939-6320-1Published: 23 August 2016

  • eBook ISBN: 978-1-62703-459-3Published: 25 May 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 249

  • Number of Illustrations: 21 b/w illustrations, 25 illustrations in colour

  • Topics: Biochemistry, general

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