Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1555)
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About this book
This volume covers an array of techniques available for studying SH2 domains and phosphotyrosine signaling. The book is divided into six parts: Part I outlines the history of SH2, technology development, and cell signaling; Part II focuses on computational approaches and tools used for identification, classification, and predictions of SH2 domain binding partners; Part III details various ways to prepare the SH2 domains as experimental reagents; Part IV presents methods for structural analysis and conventional binding assays using SH2 domains; Part V describes high-throughput and proteomics approaches to aid in analyzing SH2-mediated interactions; and Part VI covers applications for SH2 domain to functional and imaging analyzes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, SH2 Domains: Methods and Protocols is a valuable resource for computational biologists, biochemists, structural biologists, cell biologists, pathologists, and people interested in SH2 domains and phosphotyrosine signaling. Researchers who are investigating how protein interaction domain mediate specificity in signaling systems may also find this book informative.
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Keywords
Table of contents (31 protocols)
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Overview
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Computational Approaches
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Experimental Tools
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Structural Analysis and Binding Assays
Editors and Affiliations
About the editors
Tel: 860-679-1830; Email: machida@neuron.uchc.edu
Bibliographic Information
Book Title: SH2 Domains
Book Subtitle: Methods and Protocols
Editors: Kazuya Machida, Bernard A. Liu
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6762-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6760-5Published: 15 January 2017
Softcover ISBN: 978-1-4939-8295-0Published: 09 June 2018
eBook ISBN: 978-1-4939-6762-9Published: 14 January 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 558
Number of Illustrations: 39 b/w illustrations, 79 illustrations in colour
Topics: Protein Science