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SH2 Domains pp 257-267 | Cite as

Structural Characterization of Monomeric/Dimeric State of p59fyn SH2 Domain

  • Radu Huculeci
  • Fabien Kieken
  • Abel Garcia-Pino
  • Lieven Buts
  • Nico van Nuland
  • Tom Lenaerts
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1555)

Abstract

Src homology 2 (SH2) domains are key modulators in various signaling pathways allowing the recognition of phosphotyrosine sites of different proteins. Despite the fact that SH2 domains acquire their biological functions in a monomeric state, a multitude of reports have shown their tendency to dimerize. Here, we provide a technical description on how to isolate and characterize by gel filtration, circular dichroism (CD), and nuclear magnetic resonance (NMR) each conformational state of p59fyn SH2 domain.

Key words

SH2 domain Monomer/dimer Gel filtration chromatography Circular dichroism (CD) Nuclear magnetic resonance (NMR) 

Notes

Acknowledgments

R.H., F.K., Nv.N., L.B., A.G., and T.L. were supported by Fonds Wetenschappelijk Onderzoek (FWO) via research grants G.0.116.09.N.10 and G025915N. Additional funding for T.L. was provided by Fondation de la Recherche Scientifique (FRS-FNRS) via the FRFC grant 2.4606.11. The VIB and the Hercules Foundation support the research in Nv.N. group.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Radu Huculeci
    • 1
    • 2
  • Fabien Kieken
    • 1
    • 2
  • Abel Garcia-Pino
    • 1
    • 2
  • Lieven Buts
    • 1
    • 2
  • Nico van Nuland
    • 1
    • 2
  • Tom Lenaerts
    • 3
    • 4
    • 5
  1. 1.Jean Jeener NMR Center, Structural Biology BrusselsVrije Universiteit BrusselBrusselBelgium
  2. 2.Structural Biology Research CenterVIBBrusselBelgium
  3. 3.MLG, Département d’InformatiqueUniversité Libre de BruxellesBrusselsBelgium
  4. 4.AI-lab, Vakgroep ComputerwetenschappenVrije Universiteit BrusselBrusselBelgium
  5. 5.Interuniversity Institute of Bioinformatics Brussels (IB2)ULB-VUBBrusselsBelgium

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