Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1276)
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About this book
This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls.
Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future.
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Keywords
Table of contents (20 protocols)
Editors and Affiliations
About the editors
Bibliographic Information
Book Title: Bacterial Transcriptional Control
Book Subtitle: Methods and Protocols
Editors: Irina Artsimovitch, Thomas J. Santangelo
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2392-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2391-5Published: 10 February 2015
Softcover ISBN: 978-1-4939-5467-4Published: 05 October 2016
eBook ISBN: 978-1-4939-2392-2Published: 09 February 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 342
Number of Illustrations: 23 b/w illustrations, 25 illustrations in colour
Topics: Microbiology, Bacteriology, Genetic Engineering