Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1227)
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Table of contents (18 protocols)
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BAC Construction and Characterization
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Modification of BACs
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Gene Expression Using BACs as Vectors
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Applications of BACs in Model Organisms, Medical Genetics, and Drug Discovery
Keywords
About this book
Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with BACs. Updated chapters included in this edition present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model organisms, and medical genetics and drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and tips to troubleshoot and avoid known pitfalls.
Authoritative and cutting-edge, Bacterial Artificial Chromosomes, Second Edition seeks to aid scientists in advancing their research using these exciting BAC techniques and strategies.
Editors and Affiliations
Bibliographic Information
Book Title: Bacterial Artificial Chromosomes
Editors: Kumaran Narayanan
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1652-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-1651-1Published: 20 September 2014
Softcover ISBN: 978-1-4939-5430-8Published: 22 September 2016
eBook ISBN: 978-1-4939-1652-8Published: 19 September 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 348
Number of Illustrations: 12 b/w illustrations, 36 illustrations in colour
Topics: Cell Biology, Gene Function