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Bidimensional Analyses of the Intra- and Extracellular Proteomes of Steroid Producer Mycobacteria

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Microbial Steroids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2704))

Abstract

The importance of the pathogenic mycobacteria has mainly focused the omic analyses on different aspects of their clinical significance. However, those industrially relevant mycobacteria have received less attention, even though the steroid market sales in 2021 were estimated in $56.45 billion.

The extracellular proteome, due to its relevance in the sterol processing and uptake, and the intracellular proteome, because of its role in steroids bioconversion, are the core of the present chapter. Both, monodimensional gels, as preparatory analysis, and bidimensional gels as proteome analysis are described. As a proof of concept, the protein extraction methods for both sub-proteomes of Mycobacterium are described. Thus, procedures and relevant key points of these proteome analyses are fully detailed.

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Acknowledgments

Special thanks to (a) the ESTELLA project (“DESign of bio-based Thermoset polymer with rEcycLing capabiLity by dynAmic bonds for bio-composite manufacturing”) (Project no. 101058371) funded by the European Union through the Horizon Europe Framework Programme (call: HORIZON-CL4-2021-RESILIENCE-01-11) and (b) the BioPac project (Development of bioactive and lifespan-controlled bioplastics) (Ref. no. TED2021-131864B-C21) funded by the MCIN (Ministerio de Ciencia e Innovación)/AEI (Agencia Estatal de Investigación)/10.13039/501100011033 (Digital Object Identifier) and the European Union “NextGenerationEU”/PRTR (Recovery, Transformation and Resilience Plan).

Ana Ibañez is supported by a “Margarita Salas” modality postdoctoral grant (Reference no. UP2021-025) through the University of León awarded by the Spanish Ministry of Universities within the Recovery, Transformation and Resilience Plan (Modernization and Digitalization of the Educational System), the funding of which comes from the European Recovery Instrument European Union-NextGenerationEU.

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Authors and Affiliations

  1. Área de Bioquímica y Biología Molecular, Departamento de Biología Molecular, Facultad de Veterinaria, Universidad de León, León, Spain

    Carlos Barreiro

  2. Instituto de Investigación de la Viña y el Vino, Escuela de Ingeniería Agraria, Universidad de León, León, Spain

    Ana M. Ibáñez

  3. Instituto Tecnológico Agrario de Castilla y León (ITACyL), Área de Investigación Agrícola, Valladolid, Spain

    Ana M. Ibáñez

Authors
  1. Carlos Barreiro
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  2. Ana M. Ibáñez
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Correspondence to Carlos Barreiro .

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Editors and Affiliations

  1. Área de Bioquímica y Biología Molecular, Departamento de Biología Molecular, Facultad de Veterinaria, Universidad de León, León, Spain

    Carlos Barreiro

  2. Department of Biotechnology, Curia Spain, León, Spain

    José-Luis Barredo

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Barreiro, C., Ibáñez, A.M. (2023). Bidimensional Analyses of the Intra- and Extracellular Proteomes of Steroid Producer Mycobacteria. In: Barreiro, C., Barredo, JL. (eds) Microbial Steroids. Methods in Molecular Biology, vol 2704. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3385-4_7

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  • DOI: https://doi.org/10.1007/978-1-0716-3385-4_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3384-7

  • Online ISBN: 978-1-0716-3385-4

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