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Peroxisomes

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Michael Schrader

    1. Faculty of Health and Life Sciences, Biosciences, University of Exeter, Exeter, UK

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2643)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. Isolation of Mammalian Peroxisomes by Density Gradient Centrifugation

    • Andreas Manner, Markus Islinger
    Pages 1-12

  3. Analysis of Yeast Peroxisomes via Spatial Proteomics

    • Hirak Das, Alexandros Zografakis, Silke Oeljeklaus, Bettina Warscheid
    Pages 13-31

  4. Isolation of Glycosomes from Trypanosoma brucei

    • Chethan K. Krishna, Laura Franke, Ralf Erdmann, Vishal C. Kalel
    Pages 33-45

  5. Immunolabeling for Detection of Endogenous and Overexpressed Peroxisomal Proteins in Mammalian Cells

    • Tina A. Schrader, Ruth E. Carmichael, Michael Schrader
    Pages 47-63

  6. Super-Resolution Imaging of Peroxisomal Proteins Using STED Nanoscopy

    • Eline M. F. de Lange, Rifka Vlijm
    Pages 65-84

  7. Direct Stochastic Optical Reconstruction Microscopy (dSTORM) of Peroxisomes

    • Teresa Klein, Markus Sauer, Süleyman Ergün, Srikanth Karnati
    Pages 85-92

  8. Correlative Light- and Electron Microscopy in Peroxisome Research

    • Rinse de Boer, Ida J. van der Klei
    Pages 93-104

  9. Ultrastructural Analysis and Quantification of Peroxisome-Organelle Contacts

    • Christian Hacker, Tina A. Schrader, Michael Schrader
    Pages 105-122

  10. Detection of Peroxisomal Proteins During Mycobacterial Infection

    • Ananyaashree Behera, Mainak Biswas, Süleyman Ergün, Srikanth Karnati, Avinash Sonawane
    Pages 123-134

  11. Proximity-Ligation Assay to Detect Peroxisome-Organelle Interaction

    • Maki Kamoshita, Michael Schrader
    Pages 135-148

  12. Assay of Reactive Oxygen/Nitrogen Species (ROS/RNS) in Arabidopsis Peroxisomes Through Fluorescent Protein Containing a Type 1 Peroxisomal Targeting Signal (PTS1)

    • Francisco J. Corpas, José M. Palma
    Pages 149-160

  13. Identification of Peroxisome-Derived Hydrogen Peroxide-Sensitive Target Proteins Using a YAP1C-Based Genetic Probe

    • Celien Lismont, Iulia Revenco, Cláudio F. Costa, Hongli Li, Mohamed A. F. Hussein, Paul P. Van Veldhoven et al.
    Pages 161-181

  14. Assessment of the Peroxisomal Redox State in Living Cells Using NADPH- and NAD+/NADH-Specific Fluorescent Protein Sensors

    • Cláudio F. Costa, Hongli Li, Mohamed A. F. Hussein, Yi Yang, Celien Lismont, Marc Fransen
    Pages 183-197

  15. Live-Cell Imaging of Peroxisomal Calcium Levels and Dynamics

    • Yelena Sargsyan, Sven Thoms
    Pages 199-206

  16. Analysis of Peroxisome Biogenesis by Phos-Tag SDS-PAGE

    • Kanji Okumoto, Yukio Fujiki
    Pages 207-215

  17. Targeted Modifications of the Yeast Genome to Study Peroxisomes

    • Lakhan Ekal, Ewald Hettema
    Pages 217-232

  18. Applying CRISPR-Cas9 Genome Editing to Study Genes Involved in Peroxisome Biogenesis or Peroxisomal Functions

    • Serhii Chornyi, Janet Koster, Hans R. Waterham
    Pages 233-245

  19. Generation of Reporter Cell Lines for Endogenous Expression Analysis of Peroxisomal Proteins

    • Beatriz S. C. Silva, Tina A. Schrader, Michael Schrader, Ruth E. Carmichael
    Pages 247-270

  20. Peroxisomes and Viruses: Overview on Current Knowledge and Experimental Approaches

    • Vanessa Ferreira, Ana Rita Ferreira, Daniela Ribeiro
    Pages 271-294
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Keywords

About this book

This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this dynamic organelle are still being discovered through studies in model systems including humans, mice, flies, plants, fungi, and yeast, and this progress is reflected in the chapters included in this collection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Peroxisomes: Methods and Protocols, Second Edition serves as an ideal guide for researchers working on peroxisome- and organelle-based research questions.

Editors and Affiliations

  • Faculty of Health and Life Sciences, Biosciences, University of Exeter, Exeter, UK

    Michael Schrader

Bibliographic Information

  • Book Title: Peroxisomes

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Schrader

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3048-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3047-1Published: 24 March 2023

  • Softcover ISBN: 978-1-0716-3050-1Published: 23 March 2023

  • eBook ISBN: 978-1-0716-3048-8Published: 23 March 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVII, 507

  • Number of Illustrations: 22 b/w illustrations, 66 illustrations in colour

  • Topics: Cell Biology, Cell Biology

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