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Proximity-Ligation Assay to Detect Peroxisome-Organelle Interaction

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Peroxisomes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2643))

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Abstract

Peroxisomes are essential organelles in mammals, which contribute to cellular lipid metabolism and redox homeostasis. They do not function as isolated entities but cooperate and interact with other subcellular organelles, in particular the endoplasmic reticulum, mitochondria, and lipid droplets. Those interactions are often mediated by membrane contact sites. Tether proteins at those sites bring the organelles in close proximity to facilitate metabolite and lipid transfer as well as organelle communication. There is great interest in the investigation of the physiological functions of peroxisome-organelle contacts and how they are regulated. Here, we present an antibody- and fluorescence-based proximity ligation approach used successfully in our laboratory for the detection and quantification of peroxisome-organelle interactions in cultured mammalian cells.

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Acknowledgments

We would like to thank Tina A. Schrader for critical reading of the manuscript and helpful comments. This work was in part supported by the Biotechnology and Biological Sciences Research Council (BB/R016844/1; BB/T002255/1).

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Authors and Affiliations

  1. Faculty of Health and Life Sciences, Biosciences, University of Exeter, Exeter, Devon, UK

    Maki Kamoshita & Michael Schrader

  2. Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan

    Maki Kamoshita

Authors
  1. Maki Kamoshita
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  2. Michael Schrader
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Corresponding author

Correspondence to Michael Schrader .

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Editors and Affiliations

  1. Faculty of Health and Life Sciences, Biosciences, University of Exeter, Exeter, Devon, UK

    Michael Schrader

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kamoshita, M., Schrader, M. (2023). Proximity-Ligation Assay to Detect Peroxisome-Organelle Interaction. In: Schrader, M. (eds) Peroxisomes. Methods in Molecular Biology, vol 2643. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3048-8_10

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  • DOI: https://doi.org/10.1007/978-1-0716-3048-8_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3047-1

  • Online ISBN: 978-1-0716-3048-8

  • eBook Packages: Springer Protocols

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