Abstract
Stress-induced methylglyoxal (MG) functions as a toxic molecule, inhibiting plant physiological processes such as photosynthesis and antioxidant defense systems. In the present study, an attempt was made to investigate the MG detoxification through glutathione metabolism in indica rice [Oryza sativa L. ssp. indica cv. Pathumthani 1] under salt stress by exogenous foliar application of paclobutrazol (PBZ). Fourteen-day-old rice seedlings were pretreated with 15 mg L−1 PBZ foliar spray. After 7 days, rice seedlings were subsequently exposed to 0 (control) or 150 mM NaCl (salt stress) for 12 days. Prolonged salt stress enhanced the production of MG molecules and the oxidation of proteins, leading to decreased activity of glyoxalase enzymes, glyoxalase I (Gly I) and glyoxalase II (Gly II). Consequently, the decreased glyoxalase activities were also associated with a decline in reduced glutathione (GSH) content and glutathione reductase (GR) activity. PBZ pretreatment of rice seedlings under salt stress significantly lowered MG production and protein oxidation, and increased the activities of both Gly I and Gly II. PBZ also increased GSH content and GR activity along with the up-regulation of glyoxalase enzymes, under salt stress. In summary, salinity induced a high level of MG and the associated oxidative damage, while PBZ application reduced the MG toxicity by up-regulating glyoxalase and glutathione defense system in rice seedlings.
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Abbreviations
- Gly I:
-
Glyoxalase I
- Gly II:
-
Glyoxalase II
- GR:
-
Glutathione reductase
- GSH:
-
Reduced glutathione
- GSSG:
-
Oxidized glutathione
- MG:
-
Methylglyoxal
- PBZ:
-
Paclobutrazol
- PTT1:
-
Pathumthani 1
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Acknowledgments
The authors would like to thank Chiang Mai University for granting support and partially support by the Thailand Graduate Institute of Science and Technology (TGIST), National Science and Technology Development Agency (NSTDA) Grant (granting number TG-22-10-55-005D) for BK as doctoral program.
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Khunpon, B., Cha-um, S., Faiyue, B. et al. Paclobutrazol mitigates salt stress in indica rice seedlings by enhancing glutathione metabolism and glyoxalase system. Biologia 73, 1267–1276 (2018). https://doi.org/10.2478/s11756-018-0132-4
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DOI: https://doi.org/10.2478/s11756-018-0132-4