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Nanoparticle-based Point of Care Immunoassays for in vitro Biomedical Diagnostics

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Abstract

In resource-limited settings, the availability of medical practitioners and early diagnostic facilities are inadequate relative to the population size and disease burden. To address cost and delayed time issues in diagnostics, strip-based immunoassays, e.g. dipstick, lateral flow assay (LFA) and microfluidic paper-based analytical devices (microPADs), have emerged as promising alternatives to conventional diagnostic approaches. These assays rely on chromogenic agents to detect disease biomarkers. However, limited specificity and sensitivity have motivated scientists to improve the efficiency of these assays by conjugating chromogenic agents with nanoparticles for enhanced qualitative and quantitative output. Various nanomaterials, which include metallic, magnetic and luminescent nanoparticles, are being used in the fabrication of biosensors to detect and quantify biomolecules and disease biomarkers. This review discusses some of the principles and applications of such nanoparticle-based point of care biosensors in biomedical diagnosis.

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References

  1. W. G. Lee, Y. G. Kim, B. G. Chung, U. Demirci, and A. Khademhosseini, Adv. Drug Delivery Rev., 2010, 62, 449.

    Article  CAS  Google Scholar 

  2. A. Johannessen, J. Viral Hepatitis, 2015, 22, 362.

    Article  CAS  Google Scholar 

  3. C. Briggs, S. Kimber, and L. Green, Br. J. Haematol., 2012, 158, 679.

    Article  PubMed  Google Scholar 

  4. D. A. Giljohann and C. A. Mirkin, Nature, 2009, 462, 461.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. K. Mahato, A. Srivastava, and P. Chandra, Biosens. Bioelectron., 2017, 96, 246.

    Article  CAS  PubMed  Google Scholar 

  6. S. Sununta, P. Rattanarat, O. Challapakul, and N. Praphairaksit, Anal. Sci., 2018, 34, 109.

    Article  CAS  PubMed  Google Scholar 

  7. P. Worftamongkona, K. Seeda, P. Phansomboon, N. Ratnarathorn, O. Chailapakul, and W. Dungghai, Anal. Sci., 2018, 34, 103.

    Article  Google Scholar 

  8. H. Hisamoto, Anal. Sci., 2017, 33, 753.

    Article  CAS  PubMed  Google Scholar 

  9. T. Komatsu, M. Maeki, A. Ishida, H. Tani, and M. Tokeshi, Anal. Sci., 2018, 34, 39.

    Article  CAS  PubMed  Google Scholar 

  10. V. B. C. Lee, N. F. Mom-Mum, E. Tamiya, and M. U. Ahmed, Anal. Sci., 2018, 34, 7.

    Article  CAS  PubMed  Google Scholar 

  11. C. Schmidt and J. Storsberg, Biomedicines, 2015, 3, 203.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. W. Zhou, X. Gao, D. Liu, and X. Chen, Chem. Rev., 2015, 115, 10575.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. E. Kazura, B. R. Lubbers, E. Dawson, J. A. Phillips and F. Baudenbacher, Biomed. Microdevices, 2017, 19.

    Google Scholar 

  14. G. C. Ilacas, A. Basa, A. Sen and F. A. Gomez, Anal. Sci., 2018, 34, 115.

    Article  CAS  PubMed  Google Scholar 

  15. M. Ishii, P. Preechakasedkit, K. Yamada, O. Chailapakul, K. Suzuki, and D. Citterio, Anal. Sci., 2018, 34, 51.

    Article  CAS  PubMed  Google Scholar 

  16. V. X. G. Oliveira, A. A. Dias, L. L. Carvalho, T. M. G. Cardoso, F. Colmati, and W. K. T. Coltro, Anal. Sci., 2018, 34, 91.

    Article  CAS  PubMed  Google Scholar 

  17. Y. He, S. Zhang, X. Zhang, M. Baloda, A. S. Gurung, H. Xu, X. Zhang, and G. Liu, Biosens. Bioelectron., 2011, 26, 2018.

    Article  CAS  PubMed  Google Scholar 

  18. L. Tang and J. Li, ACS Sens., 2017, 2, 857.

    Article  CAS  PubMed  Google Scholar 

  19. X. Zhang, A. Zambrano, Z. T. Lin, Y. Xing, J. Rippy, and T. Wu, Arch. Immunol. Ther. Exp. (Warsz.), 2017, 65, 111.

    Article  CAS  PubMed  Google Scholar 

  20. K. A. Kirk, A. Othman, and S. Andreescu, Anal. Sci., 2018, 34, 19.

    Article  CAS  PubMed  Google Scholar 

  21. T. S. Hauck, S. Giri, Y. Gao, and W. C. Chan, Adv. Drug Delivery Rev., 2010, 62, 438.

    Article  CAS  Google Scholar 

  22. T. Piyanan, A. Athipornchai, C. S. Henry, and Y. Sameenoi, Anal. Sci., 2018, 34, 97.

    Article  CAS  PubMed  Google Scholar 

  23. K. Sato, K. Hosokawa, and M. Maeda, Anal. Sci., 2007, 23, 17.

    Article  PubMed  Google Scholar 

  24. M. Larguinho and P. V. Baptista, J. Proteomics, 2012, 75, 2811.

    Article  CAS  PubMed  Google Scholar 

  25. P. Baptista, E. Pereira, P. Eaton, G. Doria, A. Miranda, I. Gomes, P. Quaresma, and R. Franco, Anal. Bioanal. Chem., 2008, 391, 943.

    Article  CAS  PubMed  Google Scholar 

  26. S. K. Ghosh and T. Pal, Chem. Rev., 2007, 107, 4797.

    Article  CAS  PubMed  Google Scholar 

  27. W. Chen, T. Li, S. He, D. Liu, Z. Wang, W. Zhang, and X. Jiang, Sci. China Chem., 2011, 54, 1227.

    Article  CAS  Google Scholar 

  28. W. Zhao, M. M. Ali, S. D. Aguirre, M. A. Brook, and Y. Li, Anal. Chem., 2008, 80, 8431.

    Article  CAS  PubMed  Google Scholar 

  29. C. Rivet, H. Lee, A. Hirsch, S. Hamilton, and H. Lu, Chem. Eng. Sci., 2011, 66, 1490.

    Article  CAS  Google Scholar 

  30. X. J. Xie, W. Xu, and X. G. Liu, Acc. Chem. Res., 2012, 45, 1511.

    Article  CAS  PubMed  Google Scholar 

  31. J. S. Sun, Y. L. Xianyu, and X. Y. Jiang, Chem. Soc. Rev., 2014, 43, 6239.

    Article  CAS  PubMed  Google Scholar 

  32. Y. Wang and S. R. Nugen, Biomed. Microdevices, 2013, 15, 751.

    Article  CAS  PubMed  Google Scholar 

  33. Y. Wang, C. Fill, and S. R. Nugen, Biosensors (Basel), 2012, 2, 32.

    Article  CAS  PubMed  Google Scholar 

  34. X. Huang, Y. Liu, B. Yung, Y. Xiong, and X. Chen, ACS Nano, 2017, 11, 5238.

    Article  CAS  PubMed  Google Scholar 

  35. N. Uehara, Anal. Sci., 2010, 26, 1219.

    Article  CAS  PubMed  Google Scholar 

  36. P. Costa, A. Amaro, A. Botelho, J. Inacio, and P. V. Baptista, Clin. Microbiol. Infect., 2010, 16, 1464.

    Article  CAS  PubMed  Google Scholar 

  37. T.-T. Tsai, S.-W. Shen, C.-M. Cheng and C.-F. Chen, Sci. Technol. Adv. Mater., 2013, 14, 044404.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. B. A. Rohrman, V. Leautaud, E. Molyneux, and R. R. Richards-Kortum, PLoS One, 2012, 7, e45611.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. R. H. Tang, H. Yang, J. R. Choi, Y. Gong, J. Hu, S. S. Feng, B. Pingguan-Murphy, Q. B. Mei, and F. Xu, Talanta, 2016, 152, 269.

    Article  CAS  PubMed  Google Scholar 

  40. Y. Gao, X. L. Deng, W. Wen, X. H. Zhang, and S. F. Wang, Biosens. Bioelectron., 2017, 92, 529.

    Article  CAS  PubMed  Google Scholar 

  41. X. Fu, Z. Cheng, J. Yu, P. Choo, L. Chen, and J. Choo, Biosens. Bioelectron., 2016, 78, 530.

    Article  CAS  PubMed  Google Scholar 

  42. D. S. Kim, Y. T. Kim, S. B. Hong, J. Kim, N. S. Huh, M. K. Lee, S. J. Lee, B. I. Kim, I. S. Kim, Y. S. Huh, and B. G. Choi, Sensors (Basel), 2016, 16.

    Google Scholar 

  43. R. Chapman, Y. Lin, M. Burnapp, A. Bentham, D. Hillier, A. Zabron, S. Khan, M. Tyreman, and M. M. Stevens, ACS Nano, 2015, 9, 2565.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. W. Kim, J. C. Lee, J. H. Shin, K. H. Jin, H. K. Park, and S. Choi, Anal. Chem., 2016, 88, 5531.

    Article  CAS  PubMed  Google Scholar 

  45. A. Hemben, J. Ashley, and I. E. Tothill, Biosensors (Basel), 2017, 7.

    Google Scholar 

  46. A. Chua, C. Y. Yean, M. Ravichandran, B. Lim, and P. Lalitha, Biosens. Bioelectron., 2011, 26, 3825.

    Article  CAS  PubMed  Google Scholar 

  47. P. Liang, H. Yu, B. Guntupalli, and Y. Xiao, ACS Appl. Mater. Interfaces, 2015, 7, 15023.

    Article  CAS  PubMed  Google Scholar 

  48. A. S. Z. Abidin, R. A. Rahim, M. K. M. Arshad, M. F. F. Nabilah, C. H. Voon, T. H. Tang, and M. Citartan, Sensors (Basel), 2017, 17.

    Google Scholar 

  49. P. Preechakasedkit, K. Pinwattana, W. Dungchai, W. Siangproh, W. Chaicumpa, P. Tongtawe, and O. Chailapakul, Biosens. Bioelectron., 2012, 31, 562.

    Article  CAS  PubMed  Google Scholar 

  50. Y. Yuan, J. Zhang, H. C. Zhang, and X. R. Yang, Analyst, 2012, 137, 496.

    Article  CAS  PubMed  Google Scholar 

  51. D. Vilela, M. C. González, and A. Escarpa, Anal. Chim. Acta, 2012, 751, 24.

    Article  CAS  PubMed  Google Scholar 

  52. S. Lathwal and H. D. Sikes, Lab Chip, 2016, 16, 1374.

    Article  CAS  PubMed  Google Scholar 

  53. S. Nantaphol, O. Chailapakul, and W. Siangproh, Anal. Chim. Acta, 2015, 891, 136.

    Article  CAS  PubMed  Google Scholar 

  54. H. Wei, C. Chen, B. Han, and E. Wang, Anal. Chem., 2008, 80, 7051.

    Article  CAS  PubMed  Google Scholar 

  55. W. Kim, J. C. Lee, G. J. Lee, H. K. Park, A. Lee, and S. Choi, Anal. Chem., 2017, 89, 6448.

    Article  CAS  PubMed  Google Scholar 

  56. W. Leesutthiphonchai, W. Dungchai, W. Siangproh, N. Ngamrojnavanich, and O. Chailapakul, Talanta, 2011, 85, 870.

    Article  CAS  PubMed  Google Scholar 

  57. C. P. Han, K. Xu, Q. Liu, X. H. Liu, and J. J. Li, Sens. Actuators, B, 2014, 202, 574.

    Article  CAS  Google Scholar 

  58. P. Miao, T. Liu, X. X. Li, L. M. Ning, J. Yin, and K. Han, Biosens. Bioelectron., 2013, 49, 20.

    Article  CAS  PubMed  Google Scholar 

  59. L. J. Zhao, R. J. Yu, W. Ma, H. X. Han, H. Tian, R. C. Qian, and Y. T. Long, Theranostics, 2017, 7, 876.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  60. M. M. Gao, R. Lin, L. L. Li, L. Jiang, B. F. Ye, H. He and L. L. Qiu, Spectrochim. Acta, Part A, 2014, 126, 178.

    Article  CAS  Google Scholar 

  61. Y. X. Li, M. Hong, Y. Q. Lin, Q. Bin, Z. Y. Lin, Z. W. Cai, and G. N. Chen, Chem. Commun., 2012, 48, 6562.

    Article  CAS  Google Scholar 

  62. H. B. Wang, H. D. Zhang, Y. Chen, and Y. M. Liu, Biosens. Bioelectron., 2015, 74, 581.

    Article  CAS  PubMed  Google Scholar 

  63. S. G. Ge, L. Ge, M. Yan, X. R. Song, J. H. Yu, and S. S. Liu, Biosens. Bioelectron., 2013, 43, 425.

    Article  CAS  PubMed  Google Scholar 

  64. T. Jiang, Y. Song, D. Du, X. Liu, and Y. Lin, ACS Sensors, 2016, 1, 717.

    Article  CAS  Google Scholar 

  65. F. Liu, H. Zhang, Z. Wu, H. Dong, L. Zhou, D. Yang, Y. Ge, C. Jia, H. Liu, Q. Jin, J. Zhao, Q. Zhang, and H. Mao, Talanta, 2016, 161, 205.

    Article  CAS  PubMed  Google Scholar 

  66. C. X. Liu, H. Y. Cai, J. Jia, T. Y. Cao, C. H. Xu, and C. Liu, Technol. Health Care, 2017, 25, S151.

    Article  Google Scholar 

  67. S. Wignarajah, G. A. R. Y. Suaifan, S. Bizzarro, F. J. Bikker, W. E. Kaman, and M. Zourob, Anal. Chem., 2015, 87, 12161.

    Article  CAS  PubMed  Google Scholar 

  68. W. L. Hui, S. N. Zhang, C. Zhang, Y. S. Wan, J. L. Zhu, G. Zhao, S. D. Wu, D. J. Xi, Q. L. Zhang, N. N. Li, and Y. L. Cui, Nanoscale, 2016, 8, 3579.

    Article  CAS  PubMed  Google Scholar 

  69. Y. R. Chen, K. Wang, Z. R. Liu, R. J. Sun, D. X. Cui, and J. H. He, Anal. Bioanal. Chem., 2016, 408, 2319.

    Article  CAS  PubMed  Google Scholar 

  70. T. Kokalj, E. Perez-Ruiz, and J. Lammertyn, Nat. Biotechnol., 2015, 32, 485.

    CAS  Google Scholar 

  71. S. Wang, F. Inci, G. De Libero, A. Singhal, and U. Demirci, Biotechnol. Adv., 2013, 31, 438.

    Article  PubMed  PubMed Central  Google Scholar 

  72. L. R. Goulart, C. U. Vieira, A. P. P. Freschi, F. E. Capparelli, P. T. Fujimura, J. F. Almeida, L. F. Ferreira, I. M. B. Goulart, A. G. Brito-Madurro, and J. M. Madurro, Crit. Rev. Immunol., 2010, 30, 201.

    Article  CAS  PubMed  Google Scholar 

  73. A. D. Warren, G. A. Kwong, D. K. Wood, K. Y. Lin, and S. N. Bhatia, Proc. Natl. Acad. Sci. U. S. A., 2014, 111, 3671.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  74. F. Figueredo, P. T. Garcia, E. Cortón, and W. K. T. Coltro, ACS Appl. Mater. Interfaces, 2016, 8, 11.

    Article  CAS  PubMed  Google Scholar 

  75. K. Oh, Y. S. Choi, H. Y. Yoon, N. Park, J. Kim, and Y. K. Kim, IEEE Trans. Magn., 2014, 50, 1.

    Google Scholar 

  76. E. Evans, E. F. Gabriel, T. E. Benavidez, W. K. Tomazelli Coltro, and C. D. Garcia, Analyst, 2014, 139, 5560.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  77. E. Evans, E. F. Gabriel, W. K. Coltro, and C. D. Garcia, Analyst, 2014, 139, 2127.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  78. K. Toriyama, T. Suzuki, T. Inoue, H. Eguchi, S. Hoshi, Y. Inoue, H. Aizawa, K. Miyoshi, M. Ohkubo, E. Hiwatashi, H. Tachibana, and Y. Ohashi, J. Clin. Microbiol., 2015, 53, 273.

    Article  PubMed  Google Scholar 

  79. S. Bamrungsap, C. Apiwat, W. Chantima, T. Dharakul, and N. Wiriyachaiporn, Microchim. Acta, 2014, 181, 223.

    Article  CAS  Google Scholar 

  80. Y. J. Song, W. L. Wei, and X. G. Qu, Adv. Mater., 2011, 23, 4215.

    Article  CAS  PubMed  Google Scholar 

  81. M. Ornatska, E. Sharpe, D. Andreescu, and S. Andreescu, Anal. Chem., 2011, 83, 4273.

    Article  CAS  PubMed  Google Scholar 

  82. Q. Ju, M. O. Noor, and U. J. Krull, Analyst, 2016, 141, 2838.

    Article  CAS  PubMed  Google Scholar 

  83. E. Petryayeva and W. R. Algar, Anal. Chem., 2013, 85, 8817.

    Article  CAS  PubMed  Google Scholar 

  84. D. Geissler, L. J. Charbonniere, R. F. Ziessel, N. G. Butlin, H. G. Lohmannsroben, and N. Hildebrandt, Angew. Chem. Int. Ed., 2010, 49, 1396.

    Article  CAS  Google Scholar 

  85. S. Parveen, R. Misra, and S. K. Sahoo, Nanomed. Nanotechnol. Biol. Med., 2012, 8, 147.

    Article  CAS  Google Scholar 

  86. J. Shen, Y. Zhou, F. Fu, H. Xu, J. Lv, Y. Xiong, and A. Wang, Talanta, 2015, 142, 145.

    Article  CAS  PubMed  Google Scholar 

  87. Q. H. Yang, X. Q. Gong, T. Song, J. M. Yang, S. J. Zhu, Y. H. Li, Y. Cui, Y. X. Li, B. B. Zhang, and J. Chang, Biosens. Bioelectron., 2011, 30, 145.

    Article  CAS  PubMed  Google Scholar 

  88. M. O. Noor and U. J. Krull, Anal. Chem., 2013, 85, 7502.

    Article  CAS  PubMed  Google Scholar 

  89. S. Delkhahi, M. Rahaie, and F. Rahimi, J. Fluoresc., 2017, 27, 603.

    Article  CAS  PubMed  Google Scholar 

  90. C. Yu, F. Zeng, M. Luo, and S. Wu, Nanotechnology, 2012, 23, 305503.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, Faisalabad, Pakistan

    Sumaira Nishat, Fazli Rabbi Awan & Sadia Zafar Bajwa

  2. Pakistan Institute of Engineering and Applied Sciences (PIEAS), Nilore, Islamabad, Pakistan

    Sumaira Nishat, Fazli Rabbi Awan & Sadia Zafar Bajwa

  3. Department of Computer Science, University of Agriculture, Faisalabad, Pakistan

    Sumaira Nishat

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  1. Sumaira Nishat
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  2. Fazli Rabbi Awan
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Correspondence to Fazli Rabbi Awan.

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Nishat, S., Awan, F.R. & Bajwa, S.Z. Nanoparticle-based Point of Care Immunoassays for in vitro Biomedical Diagnostics. ANAL. SCI. 35, 123–131 (2019). https://doi.org/10.2116/analsci.18R001

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