Abstract
Hemin can improve the stress resistance of plants through the heme oxygenase system. Additionally, substances contained in plants, such as secondary metabolites, can improve stress resistance. However, few studies have explored the effects of hemin on secondary metabolite content. Therefore, the effects of hemin on saponin synthesis and the mechanism of plant injury relief by hemin in Conyza blinii were investigated in this study. Hemin treatment promoted plant growth and increased the antioxidant enzyme activity and saponin content of C. blinii under osmotic stress and cold stress. Further study showed that hemin could provide sufficient precursors for saponin synthesis by improving the photosynthetic capacity of C. blinii and increasing the gene expression of key enzymes in the saponin synthesis pathway, thus increasing the saponin content. Moreover, the promotion effect of hemin on saponin synthesis is dependent on heme oxygenase-1 and can be reversed by the inhibitor Zn-protoporphyrin-IX (ZnPPIX). This study revealed that hemin can increase the saponin content of C. blinii and alleviate the damage caused by abiotic stress, and it also broadened the understanding of the relationship between hemin and secondary metabolites in plant abiotic stress relief.
概要
目的
研究氯化血红素对金龙胆草皂苷含量的影响,以及与植物非生物胁迫的相关性。
创新点
通过施加外源氯化血红素研究金龙胆草中皂苷的变化与冷胁迫或渗透胁迫的相关性。
方法
在渗透胁迫和低温胁迫下,施加外源氯化血红素及其抑制剂,检测并分析金龙胆草多项生理指标、皂苷含量差异和关键基因表达量差异。
结论
氯化血红素能通过依赖血红素加氧酶-1的调控方式,提高金龙胆草皂苷的含量,从而减轻非生物胁迫对金龙胆草的伤害。
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This work was supported by the Sichuan Science and Technology Program (No. 2020YFH0136), China.
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Junyi ZHAN and Tianrun ZHENG designed research; Junyi ZHAN, Tianrun ZHENG, Wenjun SUN, Ming YANG, and
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Tianrun ZHENG, Junyi ZHAN, Ming YANG, Maojia WANG, Wenjun SUN, Zhi SHAN, and Hui CHEN declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.
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Zheng, T., Zhan, J., Yang, M. et al. Hemin-induced increase in saponin content contributes to the alleviation of osmotic and cold stress damage to Conyza blinii in a heme oxygenase 1-dependent manner. J. Zhejiang Univ. Sci. B 22, 682–694 (2021). https://doi.org/10.1631/jzus.B2000697
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DOI: https://doi.org/10.1631/jzus.B2000697