Abstract
Aims
Drought stress affects crop growth and productivity by causing morphological, physiological, and molecular changes. Pseudomonas koreensis is a plant-growth-promoting rhizobacterium that has potential for inducing stress tolerance in crops; however, the underlying mechanisms remain unclear. Therefore, this study verified the effects of P. koreensis on drought stress in tomato (Solanum lycopersicum Mill.) and explored the associated mechanisms.
Methods
Tomato seedlings that were exposed to the cell-free culture filtrate of P. koreensis GS or not were grown under non-stressed and drought-stressed conditions. Plant and leaf samples subjected to integrated physiological, transcriptomic, and metabolomic analyses.
Results
Under drought stress, treatment with cell-free culture filtrate prominently increased plant height (7.7%), root length (17.2%), dry biomass (19.8%), relative water content (19.2%), leaf chlorophyll content (13.6%), and net photosynthesis rate (56.5%) in tomato. The culture filtrate treatment also enhanced catalase and peroxidase activity but decreased malondialdehyde content in drought-stressed leaves. Many differentially expressed genes and accumulated metabolites associated with antioxidant defense were enriched in flavonoid biosynthesis, glutathione metabolism, porphyrin and chlorophyll metabolism, photosynthesis, and fructose and mannose metabolism, which jointly contributed to the alleviation of drought stress.
Conclusions
The enhancement of plant antioxidant capacity is the key factor for P. koreensis GS cell-free culture filtrate in alleviating drought stress in tomato.
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Acknowledgements
This research was financially supported by the Youth Project of National Natural Science Foundation of China (31600407), the Science and Technology Major Project of Ordos (2022EEDSKJZDZX019), the Science and Technology Planning Project of Inner Mongolia Autonomous Region (2022YFHH0114), the Natural Science Foundation of Shaanxi Province, China (2021JQ-151), and the Qinchuangyuan Scientific and Technological Innovation Funds of China (2021ZDZX-NY-0005).
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Guo, Q., Sun, C., Jing, Y. et al. Pseudomonas koreensis culture filtrate alleviates tomato drought stress: modulation of antioxidant systems coupled with the porphyrin and chlorophyll–photosynthesis–fructose and mannose axis. Plant Soil 484, 237–256 (2023). https://doi.org/10.1007/s11104-022-05786-7
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DOI: https://doi.org/10.1007/s11104-022-05786-7