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Development of cross-linked collagen/pullulan ocular film for sustained delivery of Besifloxacin using novel spin-coating technique

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Abstract

Spin coaters are ideal tools for the preparation of thin and ultrathin films. In the present investigation, an attempt has been made to develop a novel and simplified method for the preparation of cross-linked collagen (CCG)/pullulan film. Marine collagen (MCG) has been modified, and crosslinking efficiency was preliminarily confirmed by observing an increase in CH2–CH2 stretching using vibrational spectroscopy, dynamic light scattering, X-ray diffraction, etc. The spray drying of CCG and Besifloxacin (BFN)–CCG produced spherical-shaped particles as confirmed from surface morphological observations. The molecular weight of MCG was slightly decreased after crosslinking (CCG) and shifted from 4205 to 4112 Da indicating the copolymerization. The emergence of high intensity peak in Nuclear Magnetic Resonance at 63 ppm was assigned for covalent bond formation in CCG. The BFN–CCG pullulan film (67.38%) showed an increase in dissolution time at the end of 12 h. Developed BFN–CCG pullulan film demonstrated enhanced antibacterial effect against Staphylococcus Aureus and Escherichia Coli.

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Authors and Affiliations

  1. Department of Pharmaceutics, Dr. Rajendra Gode College of Pharmacy, Malkapur, Dist, Buldhana, M.S., 443 101, India

    Mahesh P. More & Prashant K. Deshmukh

  2. Deparment of Pharmaceutics, H. R. Patel Institute of Pharmaceutical Education and Research, Karvand Naka, Shirpur, Dist, Dhule, M.S., 425 405, India

    Shweta Patil

  3. Department of Chemical Engineering, Institute of Chemical Technology, Mumbai, 400 019, India

    Sharwari Ghodke & Ratnesh Jain

  4. Department of Pharmaceutical Chemistry, H. R. Patel Insitute of Pharmaceutical Education and Research, Karvand Naka, Shirpur, Dist, Dhule, M.S., 425 405, India

    Pravin O. Patil

  5. Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, 400 019, India

    Prajakta Dandekar

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  1. Mahesh P. More
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More, M.P., Patil, S., Ghodke, S. et al. Development of cross-linked collagen/pullulan ocular film for sustained delivery of Besifloxacin using novel spin-coating technique. Journal of Materials Research 36, 3278–3292 (2021). https://doi.org/10.1557/s43578-021-00359-3

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