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In vitro and in vivo biocompatibility of polyurethanes synthesized with castor oil polyols for biomedical devices

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Abstract

Polyurethanes (PUs) were synthesized with polyols derived from castor oil and isophorone diisocyanate. The materials were evaluated for their mechanical properties using stress—strain curves, thermogravimetric analysis, differential scanning calorimetry, and contact angle analysis. The biological response of the materials was evaluated by determining their cell viability in vitro, antimicrobial activity against Escherichia coli and Pseudomonas aeruginosa, and biological response in vivo of PUs by means of implanting them in Wistar rats. The cell proliferation on the materials was analyzed using mouse fibroblast L929, human fibroblast MRC-5, and adult human dermal fibroblast (HDFa) cells by the ISO 10993-5 method. The materials showed no toxic effects and promoted cell proliferation. Experiments performed in vivo for 30 days in mice showed that the materials neither affected the wound healing process nor caused adverse effects or severe injuries in the dorsal mid-cervical tissue or organs on histological evaluation. PUs synthesized can be used in biomedical devices.

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Acknowledgments

We would like to thank the Universidad de La Sabana for financing the ING-176-2016 research project and Colciencias for the doctoral grant-loan with the call number 617-2 in 2014. We would also like to thank the Bioterium of the National Institute of Health of Colombia and the Corporación Patología Veterinaria (Corpavet®) for collaboration in conducting the in vivo assays.

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Authors and Affiliations

  1. Doctoral Program in Bioscience, Research Group on Energy, Materials and Environment, Universidad de La Sabana, Chía, Cundinamarca, 53753, Colombia

    Yomaira L. Uscátegui

  2. Research Group on Bioprospecting, Universidad de La Sabana, Chía, Cundinamarca, 53753, Colombia

    Luis E. Díaz

  3. Chemical Engineering Program, Research Group on Energy, Materials and Environment, Universidad de La Sabana, Chía, Cundinamarca, 53753, Colombia

    Manuel F. Valero

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  1. Yomaira L. Uscátegui
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Correspondence to Manuel F. Valero.

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Uscátegui, Y.L., Díaz, L.E. & Valero, M.F. In vitro and in vivo biocompatibility of polyurethanes synthesized with castor oil polyols for biomedical devices. Journal of Materials Research 34, 519–531 (2019). https://doi.org/10.1557/jmr.2018.448

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