Abstract
Semi-dwarf varieties in wheat associated with gibberellic acid (GA3)-insensitive height reducing genes have led to significant increases in yield but often fall below this potential because of poor seedling emergence after deep showing. Alternative semi-dwarf genes may have the potential to reduce plant height without compromising early plant growth. In durum wheat, bulk segregant analysis was used to screen microsatellite markers linked with the GA3-sensitive genes Rht14 in cv. Castelporziano, Rht16 in cv. Edmore M1 and Rht18 in cv. Icaro. Molecular marker Xbarc3-6A for Rht14, Rht16 and Rht18 showed significant polymorphic differences among DNA bulks for height classes. The genes Rht14, Rht16 and Rht18 were linked with Xbarc3 (11.7–28.0 cM) on the short arm of chromosome 6A and they appear to be allelic. Semi-dwarf genes on chromosome 6AS may potentially be used in breeding for improved establishment.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Addisu, M, Snape, J.W., Simmonds, J.R., Gooding, M.J. 2009. Reduced height (Rht) and photoperiod insensitivety (Ppd) allele associations with establishment and early growth of wheat in contrasting production system. Euphytica 166:249–267.
Dellaporta, S.L., Wood, J., Hicks, J.B. 1983. A plant DNA mini preparation: Version II. Plant Mol. Biol. Rep. 1:19–21.
Ellis, M.H., Rebetzke, G.J., Azanza, F., Richards, R.A., Spielmeyer, W. 2005. Molecular mapping of gibberellin-responsive dwarfing genes in bread wheat. Theor. Appl. Genet. 111:423–430.
Ellis, M.H., Rebetzke, G.J., Chandler, P., Bonnett, D.G., Spielmeyer, W., Richards, R.A. 2004. The effect of different height reducing genes on the early growth of wheat. Funct. Plant Biol. 31:583–589.
Evans, L.T. 1993. Crop Evolution, Adaptation, and Yield. Cambridge University Press, Cambridge, UK.
Gale, M.D., Youssefian, S. 1985. Dwarfing genes in wheat. In: Russell, G.E. (ed.), Progress in Plant Breeding. Butterworths, London, UK, pp. 1–35.
Giorgi, B., Barbera, F., Bitti, O., Cavicchioni, G. 1984. Yield performance of F3 progenies from a durum wheat involving two different semidwarfing genes: Rht1 and Sd mutation. In: Semi-dwarf Cereal Mutants and Their Use in Cross-breeding II. Research Coordination Meeting 1982. IAEA, Vienna, Austria, pp. 91–95.
Konzak, C.F. 1988. Genetic analysis, genetic improvement and evaluation of induced semi-dwarf mutants in wheat. In: Semi-dwarf Cereal Mutants and Their Use in Cross-breeding III. Research Coordination Meeting 1985. IAEA, Vienna, Austria, pp. 77–94.
Konzak, C.F., Wilson, M.R., Franks, P.A. 1984. Progress in the evaluation, use in breeding, and genetic analysis of semidwarf mutants of wheat. In: Semi-dwarf Cereal Mutants and Their Use in Cross-breeding II. Research Coordination Meeting 1982. IAEA, Vienna, Austria, pp. 39–50.
Kosambi, D.D. 1944. The estimation of map distances from recombination values. Ann. Eugen. 12:172–175.
Kosuge, K., Watanabe, N., Kuboyama, T., Melnik, V.M., Yanchenko, V.I., Rosova, M.A., Goncharov, N.P. 2008. Cytological and microsatellite mapping of mutant genes for spherical grain and compact spikes in durum wheat. Euphytica 159:289–296.
Laing, D.R., Fischer, R.A. 1977. Adaptation of semidwarf wheat cultivars to rainfed conditions. Euphytica 26:129–139.
Michelmore, R.W., Para, I., Kesselli, R.V. 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis — a rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Nat. Acad. Sci. USA 88:9828–9832.
Perry, M.W., D’Antuono, M.F. 1989. Yield improvement and associated characteristics of some Australian spring wheat cultivars introduced between 1860 and 1982. Aust. J. Agric. Res. 40:457–472.
Rebetzke, G.J., Richards, R.A., Fischer, V.M., Mickelson, B.J. 1999. Breeding long coleoptile, reduced height wheats. Euphytica 106:158–168.
Rebetzke, G.J., Richards, R.A., Sirault, X.R.R., Morrison, A.D. 2004. Genetic analysis of coleoptile length and diameter of wheat. Aust. J. Agric. Res. 55:733–743.
Rebetzke, G.J., Bruce, S.E., Kirkegaard, J.A. 2005. Longer coleoptiles improve emergence through crop residues to increase seedling number and biomass in wheat (Triticum aestivum L.). Plant Soil 272:87–100.
Rebetzke, G.J., Richards, R.A., Fettell, N.A., Long, M., Condon, A.G., Forrester, R.I., Botwright, T.L. 2007. Genotypic increases in coleoptile length improves stand establishment, vigour and grain yield of deep-sown wheat. Field Crops Res. 100:10–23.
Richards, R.A. 1992. The effect of dwarfing genes in spring wheat in dry environments. II. Growth, water use and water use efficiency. Aust. J. Agric. Res. 43:529–539.
Röder, M.S., Korzun, V., Gill, B.S., Ganal, M.W. 1998. The physical mapping of microsatellite markers in wheat. Genome 41:278–283.
Schillinger, W.F., Donaldson, E., Allan, R.E., Jones, S.S. 1998. Winter wheat seedling emergence from deep sowing depths. Agron. J. 90:582–586.
Song, Q.J., Shi, J.R., Singh, S., Fikus, E.W., Costa, J.M., Lewis, J., Gill, B.S., Ward, R., Cregan, P.B. 2005. Development and mapping of microsatellite (SSR) markers in wheat. Theor. Appl. Genet. 110:550–560.
Spielmeyer, W., Hyles, J., Joaquim, P., Azanza, F., Bonnett, D., Ellis, M.E., Moore, C., Richards, R.A. 2007. A QTL on chromosome 6A in bread wheat (Triticum aestivum) is associated with longer coleoptiles, greater seedling vigour and final plant height. Theor. Appl. Genet. 115:59–66.
Torada, A., Koike, M., Mochida, K., Ogihara, Y. 2006. SSR-based linkage map with new markers using an intraspecific population of common wheat. Theor. Appl. Genet. 112:1042–1051.
Waddington, S.R., Osmanzai, M., Yoshida, M., Ransom, J.K. 1987. The yield of durum wheats released in Mexico between 1960 and 1984. J. Agric. Sci. (Camb.) 108:469–477.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by R.A. McIntosh
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Haque, M.A., Martinek, P., Watanabe, N. et al. Genetic mapping of gibberellic acid-sensitive genes for semi-dwarfism in durum wheat. CEREAL RESEARCH COMMUNICATIONS 39, 171–178 (2011). https://doi.org/10.1556/CRC.39.2011.2.1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/CRC.39.2011.2.1