Abstract
The development of the new pharynx in anterior body fragments of G. tigrina was followed for 7 days by immunocytochemical (ICC) study using antiserum to neuropeptide F (NPF) and muscle staining with phalloidin. ICC investigation revealed the presence of NPF in pharyngeal nervous system, peripheral nerve plexuses, in central nervous system of intact planarians. NPF-immunoreactive (IR) nerve fibres were found at the site of regeneration surrounding pharyngeal rudiment. Restoration of the pharynx function during regeneration was analysed by appearance of food response in anterior fragments. Stimulating effects of NPF and FMRF on the pharyngeal regeneration has been observed. The data indicates an important role of neuropeptides in morphogenetic processes.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Brain, S. D., Cox, H. M. (2006) Neuropeptides and their receptors. Brit. J. Pharmacol.. 147, 202–211.
Brown, M. R., Crim, J. W., Arata, R. C., Cai, H. N., Chun, C., Shen, P. (1999) Identification of a Drosophila brain-gut peptide related to neuropeptide Y family. Peptides. 20, 1035–1042.
Cerstiaens, A., Benfekih, L., Zouiten, H., Verhaert, P., De Loof, A., Schoof, L. (1999) Led-NPF-1 stimulates ovarian development in locusts. Peptides. 20, 39–44.
Day, T. A., Maule, A. G. (1999) Parasitic peptides! The structure and function of neuropeptides in parasitic worms. Peptides. 20, 999–1019.
Garczynski, S., Crim, J. W., Brown, M. R. (2005) Characterization of neuropeptide F and its receptor from African malaria mosquito, Anophelis gambiae. Peptides. 26, 99–107.
Gustafsson, M. K. S., Halton, D. W., Kreshchenko, N. D., Movsessian, S. O., Raikova, O. I., Reuter, M., Terenina, N. B. (2002) Neuropeptides in flatworms. Peptides. 23, 2053–2061.
Halton, D. W., Maule, A. G. (2004) Flatworm nerve-muscle: structural and functional analysis. Can. J. Zool.. 82, 316–333.
Hrchkova, G., Velebny, S., Halton, D. W., Day, T. A., Maule, A. G. (2004) Pharmacological characterization of neuropeptide F (NPF)-induced effects on the motility of Mesocestoides corti (syn. Mesocestoides vogae) larvae. Int. J. Parasitol.. 34, 83–93.
Humphries, J. E., Kimber, M. J., Barton, Y.-W., Hsu, W., Marks, N. J., Greer, B., Harriot, P., Maule, A. G., Day, T. (2004) Structure and bioactivity of neuropeptide F from the human parasites Schistosoma mansoni and Schistosoma japonicum. J. Biol. Chem.. 279, 39880–39885.
Kreshchenko, N. D., Sheiman, I. M. (1998) The role of peripharyngeal area in regeneration of the pharynx in planarians Dugesia tigrina. Russian J. Dev. Biol.. 29, 115–120. (In Russian).
Kreshchenko, N. D., Reuter, M., Sheiman, I. M., Halton, D. W., Maule, A. G., Johnston, R. N., Shaw, C., Gustafsson, M. K. S. (1999) Relationship between musculature and nervous system in the regenerating pharynx in Girardia tigrina (Plathelminthes). Invert. Repr. Develop.. 35, 109–125.
Kreshchenko, N., Sheiman, I., Reuter, M., Gustafsson, M. K. S., Halton, D. W., Maule, A. G. (2001) Effects of FMRFamide-related peptides and Neuropeptide F on planarian regeneration (Platyhelminthes, Tricladida). Belgium J. Zool. 131(S1), 147–148.
Kreshchenko, N., Sedelnikov, Z., Sheiman, I., Reuter, M., Maule, A. G., Gustafsson, M. K. S. (2008) Effect of Neuropeptide F on planarian regeneration. Cell Tissue Res.. 331, 739–750.
Marks, N. J., Johnson, S., Halton, D. W., Shaw, C., Geary, T. G., Moore, S., Thompson, D. P. (1996) Physiological effects of platyhelminth RFamide peptides on muscle strip preparations of Fasciola hepatica (Trematoda, Digenea). Parasitology. 113, 394–401.
McVeigh, P., Kimber, M. J., Novozhilova, E., Day, T. A. (2005) Neuropeptide signaling system in flatworms. Parasitology 131S, 41–55.
Moneypenny, C. G., Kreshchenko, N., Day, T. A., Moffett, C., Halton, D. W., Maule, A. G. (2001) Physiological effects of FMRFamide-related peptides and classical transmitters on dispersed muscle fibres of the turbellarian, Procerodes littoralis. Parasitology. 122, 447–455.
Reuter, M., Gustafsson, M. K. S., Sheiman, I. M., Terenina, N., Halton, D. W., Maule, A. G., Shaw, C. (1995) The nervous system of Tricladida: An immunocytochemical study. Invertebrate Neuroscience. 1, 133–143.
Reuter, M., Sheiman, I. M., Gustafsson, M. K. S., Halton, D. W., Maule, A. G., Shaw, C. (1996) Development of the nervous system in Dugesia tigrina during regeneration after fission and decapitation. Invertebrate Reprod. Dev.. 29, 199–211.
Reuter, M., Kreshchenko, N. (2004) Flatworm asexual multiplication implicates stem cells and regeneration. Can. Zool.. 82, 334–356.
Rőszer, T., Kappelmayer, J., Nagy, G. G., Szentmiklósi, A. J., Basnakian, A. G., Bánfalvi, G. (2006) The neuropeptide FMRFamide can protect cells against apoptosis in the snail digestive gland. Apoptosis. 11, 173–182.
Salo, E. (2006) The power of regeneration and the stem-cell kingdom: freshwater planarians (Platyhelminthes). BioEssays. 28, 546–559.
Salo, E., Baguna, J. (2002) Regeneration in planarians and other worms: new findings, new tools and new perspectives. J. Exp. Zool.. 292, 528–539.
Schoof, L., Ckynen, E., Certiaens, A., Baggerman, G., Wei, Z., Vercammen, T., Nachman, R., De Loof, A., Tanaka, S. (2001) Newly discovered functions for some myotropic neuropeptides in locust. Peptides. 22, 219–227.
Sheiman, I. M., Zubina, E. V., Kreshchenko, N. D. (2002) Regulation of the feeding behavior of planarian Dugesia (Girardia) tigrina. J. Evol. Biochem. Physiol.. 38, 414–418. (In Russian)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Kreshchenko, N.D. Functions of Flatworm Neuropeptides NPF, GYIRF and FMRF in Course of Pharyngeal Regeneration of Anterior Body Fragments of Planarian, Girardia tigrina. BIOLOGIA FUTURA 59 (Suppl 2), 199–207 (2008). https://doi.org/10.1556/ABiol.59.2008.Suppl.29
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/ABiol.59.2008.Suppl.29