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Dissection of the interaction between human holo-transferrin and ciprofloxacin in the presence of silver nanoparticles: spectroscopic approaches

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Abstract

The binding of ciprofloxacin (CIP) to human holo-transferrin (HTF) in the presence of silver nanoparticles (AgNPs) has been investigated by fluorescence quenching and circular dichroism (CD) techniques as well as resonance light scattering under physiological conditions. It was determined that the intrinsic fluorescence of HTF was quenched by CIP in the presence of AgNPs through static quenching, thus confirming that a CIP-HTF complex was formed in both the binary and ternary systems. However, the analysis of HTF fluorescence quenching in these binary and ternary systems indicated that the AgNPs were affected upon complex formation between CIP and HTF and that the binding affinity between them became more substantial when the AgNPs coexisted with the drug. Fluorescence quenching proved that HTF had one class of binding sites for CIP in both binary and ternary systems. CD spectra indicated that the secondary structure of HTF changed when increasing the CIP concentration and during the simultaneous presence of CIP and AgNPs, which led to decreased contents of α-helix and β-sheet structures in HTF, inducing destabilization of the protein.

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Abbreviations

AgNPs:

silver nanoparticles

CD:

circular dichroism

CIP:

ciprofloxacin

HTF:

human holo-transferrin

REES:

red edge excitation shift

RLS:

resonance light scattering.

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Author notes

  1. Jamshidkhan Chamani

    Present address: Department of Biochemistry and Biophysics, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran

    Fatemeh Koohzad, Mahtab JahanShah-Talab, Maryam Kamshad & Reza Assaran Darban

  2. Endoscopic and Minimally Invasive Surgery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Sima Beigoli

Authors
  1. Fatemeh Koohzad
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  2. Sima Beigoli
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  3. Mahtab JahanShah-Talab
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  4. Maryam Kamshad
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  5. Reza Assaran Darban
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Correspondence to Jamshidkhan Chamani.

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Koohzad, F., Beigoli, S., JahanShah-Talab, M. et al. Dissection of the interaction between human holo-transferrin and ciprofloxacin in the presence of silver nanoparticles: spectroscopic approaches. Biologia 72, 569–580 (2017). https://doi.org/10.1515/biolog-2017-0066

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  • DOI: https://doi.org/10.1515/biolog-2017-0066

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