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Interaction of Human Serum Albumin with Indomethacin: Spectroscopic and Molecular Modeling Studies

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Abstract

In this work, the interaction between indomethacin (IM) and human serum albumin (HSA) under simulative physiological conditions was investigated by the methods of fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and molecular modeling. The experiment results showed that the fluorescence quenching of HSA by IM was a result of the formation of an IM–HSA complex and the corresponding association constants (K a) between IM and HSA at four different temperatures were determined according to the modified Stern–Volmer equation. The resulting thermodynamic parameters ΔG, ΔH, and ΔS at different temperatures indicate that the hydrophobic force plays a major role for IM–HSA association, but hydrogen bonds also could not be excluded. A molecular modeling study further confirmed the binding mode and indicated that the binding of IM to HSA primarily takes place in sub-domain IIA (site I). The conformational investigation showed that the presence of IM decreased the α-helical content of HSA and induced slight unfolding of the polypeptides of protein, which confirmed that some microenvironmental and conformational changes occur for HSA molecules.

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Acknowledgements

We gratefully acknowledge the financial support from the National Natural Science Foundation of China (Grant No. 20921062), Research Program of Hubei Province Department of Education (D20101302) and the National Innovation Experiment Program for University Students (101048902).

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Authors and Affiliations

  1. College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei, 434023, P.R. China

    Jing Zhang, Hui-Hui Sun, Ye-Zhong Zhang, Li-Yun Yang, Jie Dai & Yi Liu

  2. College of Chemistry and Molecular Sciences and State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, P.R. China

    Yi Liu

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  1. Jing Zhang
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Correspondence to Ye-Zhong Zhang or Yi Liu.

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Zhang, J., Sun, HH., Zhang, YZ. et al. Interaction of Human Serum Albumin with Indomethacin: Spectroscopic and Molecular Modeling Studies. J Solution Chem 41, 422–435 (2012). https://doi.org/10.1007/s10953-012-9809-4

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