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Anoxybacillus sp. AH1, an α-amylase-producing thermophilic bacterium isolated from Dargeçit hot spring

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Abstract

The present study was conducted to isolate α-amylase-producing thermophilic bacteria from DargeÇit hot springs in Turkey. The morphological, biochemical and physiological characterisation, as well as genetic analysis by 16S rRNA sequences indicated that the isolated strain AH1 was a member of Anoxybacillus genus. The strain was aerobe, Gram-positive and spore-forming rod, exhibiting optimum growth temperature and pH of 60°C and 7.0–7.5, respectively. Optimization of growth medium and enzyme assay conditions for extracellular α-amylase production by the novel thermophilic Anoxybacillus sp. AH1 were carried out in many different media containing a variety of carbon and nitrogen sources. Among various carbon and nitrogen sources, peptone (2054.1 U/mL) at 1% and maltose (1862.9 U/mL) at 0.5% increased α-amylase activity, compared to controls. Moreover, a high enzyme production was observed with potato starch at 0.5% and 1% (2668.4 U/mL and 3627 U/mL, respectively), as well as with 1% soluble starch (2051.9 U/mL). The enzyme activity was found to be rather high in the presence of CaCl2 up to 100 mM.

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Abbreviations

BM:

basal medium

LB:

Luria broth

NB:

nutrient broth

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Department of Biology, Molecular Biology Division, Dicle University, Science Faculty, 21280, Diyarbakır, Turkey

    Ömer Acer, Hemşe Pirinççioğlu, Fatma Matpan Bekler & Kemal Güven

  2. Ziya Gökalp Education Faculty, Science Teaching Division, Dicle University, 21280, Diyarbakır, Turkey

    Reyhan Gül-Güven

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  1. Ömer Acer
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  2. Hemşe Pirinççioğlu
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  3. Fatma Matpan Bekler
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  5. Kemal Güven
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Acer, Ö., Pirinççioğlu, H., Matpan Bekler, F. et al. Anoxybacillus sp. AH1, an α-amylase-producing thermophilic bacterium isolated from Dargeçit hot spring. Biologia 70, 853–862 (2015). https://doi.org/10.1515/biolog-2015-0111

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