Skip to main content
SpringerLink
Log in

Identification and Characterization of Novel Thermophilic Bacteria from Hot Springs, Erzurum, Turkey

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

The aim of present study was the isolation and characterization of thermophilic bacteria from three different hot springs in Erzurum, Turkey. For this purpose, 85 bacteria were isolated and characterized by ERIC-PCR genomic fingerprinting and classical identification methods such as morphological, physiological and biochemical characteristics. According to the results, 29 bacterial isolates with different band profiles were analyzed by 16S rRNA gene sequencing and identified as belonging to the genus of Bacillus, Pseudomonas, Silanimonas, Thermomonas and Thauera. This is the first report on the isolation of Silanimonas lenta, Thauera sp. and Thermomonas haemolytica from Turkey Hot Springs. The amylase, lipase and protease enzyme production potentials of the isolates were 80%, 91.25% and 81.25%, respectively. Moreover, 56.47% of the isolates (48) were able to produce all of these enzymes. Therefore, the results of the study indicated that these bacteria and their enzymes can be used as a source of industrial enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Miquel P (1888) Monographie d'u bacille vivant au-dela' de 70"centigrades. Ann Microgr 1:3–10

    Google Scholar 

  2. Brock TD, Freeze H (1969) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol 98:289–297

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Ramaley RF, Hixson J (1970) Isolation of a nonpigmented, thermophilic bacterium similar to Thermus aquaticus. J Bacteriol 103:527–528

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Oshima T, Imahori K (1974) Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int J Syst Bacteriol 24:102–112

    Article  CAS  Google Scholar 

  5. Baltaci MO, Genc B, Arslan S, Adiguzel G, Adiguzel A (2017) Isolation and characterization of thermophilic bacteria from geothermal areas in Turkey and preliminary research on biotechnologically important enzyme production. Geomicrobiol J 34(1):53–62. https://doi.org/10.1080/01490451.2015.1137662

    Article  CAS  Google Scholar 

  6. Mohammad BT, Al Daghistani HI, Jaouani A, Abdel-Latif S, Kennes C (2017) Isolation and characterization of thermophilic bacteria from Jordanian Hot Springs: Bacillus licheniformis and Thermomonas hydrothermalis isolates as potential producers of thermostable enzymes. Int J Microbiol. https://doi.org/10.1155/2017/6943952

    Article  PubMed  PubMed Central  Google Scholar 

  7. Chrisnasari R, Verina D, Tapatfeto AC, Pranata S, Patjajani T, Wahjudi M, Purwanto MGM (2018) Isolating and characterising chitinolytic thermophilic bacteria from Cangar Hot Spring, East Java. Pertanika J Trop Agric Sci 41(3):1437–1448

    Google Scholar 

  8. Stetter KO (2013) A brief history of the discovery of hyperthermophilic life. Biochem Soc Trans 41:416–420. https://doi.org/10.1042/BST20120284

    Article  PubMed  CAS  Google Scholar 

  9. Gomez J, Steiner W (2004) The biocatalytic potential of extremophiles and extremozymes. Food Technol Biotechnol 2:223–235

    Google Scholar 

  10. Meintanis C, Chalkou KI, Kormas KA et al (2008) Application of rpoB sequence similarity analysis, REP-PCR and BOX-PCR for the differentiation of species within the genus Geobacillus. Lett Appl Microbiol 46:395–401

    Article  CAS  PubMed  Google Scholar 

  11. Singh AK, Tripathi BM, Sahay H et al (2010) Biochemical and molecular characterization of thermo-alkalitolerant xylanase producing bacteria from thermal springs of Manikaran, Indian. J Microbiol 50:S2–S9

    CAS  Google Scholar 

  12. Yoneda Y, Yoshida T, Yasuda H et al. (2013) A novel thermophilic, hydrogenogenic, and carboxydotrophic bacterium Calderihabitans maritimus gen. nov., sp. nov. from a marine sediment core of an undersea caldera. Int J Syst Evol Microbiol 63:3602–3608

    Article  CAS  PubMed  Google Scholar 

  13. Cihan AC, Tekin N, Ozcan B et al (2012) The genetic diversity of genus Bacillus and the related genera revealed by 16s rRNA gene sequences and ARDRA analyses isolated from geothermal regions of Turkey. Braz J Microbiol 43:309–324

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Brock TD, Boylen KL (1973) Presence of thermophilic bacteria in laundry and domestic hot-water heaters. Appl Environ Microbiol 25(1):72–76

    Article  CAS  Google Scholar 

  15. Brock TD, Brock KM, Belly RT, Weiss RL (1972) Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch Microbiol 84(1):54–68

    CAS  Google Scholar 

  16. Strom PF (1985) Identification of thermophilic bacteria in solid-waste composting. Appl Environ Microbiol 50(4):906–913

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Slobodkin A, Campbell B, Cary SC, Bonch-Osmolovskaya E, Jeanthon C (2001) Evidence for the presence of thermophilic Fe (III)-reducing microorganisms in deep-sea hydrothermal vents at 13°N (East Pacific Rise). FEMS Microbiol Ecol 36(2–3):235–243

    PubMed  CAS  Google Scholar 

  18. Goorissen HP, Boschker HTS, Stams AJM, Hansen TA (2003) Isolation of thermophilic Desulfotomaculum strains with methanol and sulfite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum sp. nov. Int J Syst Evol Microbiol 53:1223–1229

    Article  CAS  PubMed  Google Scholar 

  19. Sako Y, Nakagawa S, Takai K, Horikoshi K (2003) Marinithermus hydrothermalis gen. nov., sp. nov., a strictly aerobic, thermophilic bacterium from a deep-sea hydrothermal vent chimney. Int J Syst Evol Microbiol 53:59–65

    Article  CAS  PubMed  Google Scholar 

  20. Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  CAS  PubMed  Google Scholar 

  21. Bae J, Rhee S, Park JR, Kim B, Park Y (2005) Isolation of uncultivated anaerobic thermophiles from compost by supplementing cell extract of Geobacillus toebii in enrichment culture medium. Extremophiles 9(6):477–485

    Article  PubMed  Google Scholar 

  22. Aanniz T, Ouadghiri M, Melloul M, Swings J, Elfahime E, Ibijbijen J, Ismaili M, Amar M (2015) Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils. Braz J Microbiol 46(2):443–453. https://doi.org/10.1590/S1517-838246220140219

    Article  PubMed  PubMed Central  Google Scholar 

  23. Adiguzel A, Ozkan H, Baris O, Inan K, Gulluce M, Sahin F (2009) Identification and characterization of thermophilic bacteria isolated from hot springs in Turkey. J Microbiol Methods 79(3):321–328. https://doi.org/10.1016/j.mimet.2009.09.026

    Article  PubMed  CAS  Google Scholar 

  24. Yilmaz B, Baltaci MO, Sisecioglu M, Adiguzel A (2015) Thermotolerant alkaline protease enzyme from Bacillus licheniformis A10: purification, characterization, effects of surfactants and organic solvents. J Enzym Inhib Med Chem 31(6):1241–1247. https://doi.org/10.3109/14756366.2015.1118687

    Article  CAS  Google Scholar 

  25. Gormez A, Sahin F, Gulluce M, Aslan I (2013) Identification and characterization of Pseudomonas syringae isolated from apricot trees in the Erzurum Province of Turkey and evaluation of cultivar reaction. Int J Plant Pathol 95(3):525–532

    Google Scholar 

  26. Harley JP, Prescott LM (2002) Laboratory exercises in Microbiology, 4th edn. The McGraw-Hill Companies, New York

    Google Scholar 

  27. Lazo GR, Roffey R, Gabriel DW (1987) Pathovars of Xanthomonas campestris are distinguishable by restriction fragment-length polymorphism. Int J Syst Evol Microbiol 37(3):214–221. https://doi.org/10.1099/00207713-37-3-214

    Article  CAS  Google Scholar 

  28. Louws FJ, Fulbright DW, Stephens CT, de Bruijn FJ (1995) Differentiation of genomic structure by rep-PCR fingerprinting to rapidly classify Xanthomonas campestris pv. vesicatoria. Phytopathol 85:528–536

    Article  CAS  Google Scholar 

  29. Louws FJ, Fulbright DW, Stephens CT, de Bruijn FJ (1994) Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Appl Environ Microbiol 60:2286–2295

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Versalovic J, Schneider M, de Bruijin FJ, Lupski JR (1994) Genomic fingerprinting of bacteria using repetitive sequence based PCR (rep-PCR). Methods Mol Cell Biol 5:25–40

    CAS  Google Scholar 

  31. Orhan F, Demircil A, Yanmis D (2017) CaCO3 and MgCO3 dissolving halophilic bacteria. Geomicrobiol J 34(9):804–810

    Article  CAS  Google Scholar 

  32. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  33. Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular evolutionary genetics analysis version 70 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Karaoglu H, Yanmis D, Gurkok S (2016) Magnesite enrichment with Pseudomonas oryzihabitans isolated from magnesite ore. Geomicrobiol J 33(1):46–51

    Article  CAS  Google Scholar 

  35. Ertugrul S, Donmez G, Takac S (2007) Isolation of lipase producing Bacillus sp from olive mill wastewater and improving its enzyme activity. J Hazard Mater 149:720–724

    Article  CAS  PubMed  Google Scholar 

  36. Oztas Gulmus E, Gormez A (2019) Characterization and biotechnological application of protease from thermophilic Thermomonas haemolytica. Arch Microbiol. https://doi.org/10.1007/s00203-019-01728-7

    Article  PubMed  Google Scholar 

  37. Yanmis D, Baltaci MO, Gulluce M, Adiguzel A (2015) Identification of thermophilic strains from geothermal areas in Turkey by using conventional and molecular techniques. Res J Biotechnol 10:39–45

    CAS  Google Scholar 

  38. Adiguzel A, İnan K, Şahin F, Arasoğlu T, Güllüce M, Beldüz AO, Barış Ö (2011) Molecular diversity of thermophilic bacteria isolated from Pasinler Hot Spring (Erzurum, Turkey). Turk J Biol 35:267–274

    CAS  Google Scholar 

  39. Bozoglu C, Hundur S, Alaylar B, Karadayi M, Gulluce M (2015) Isolation and molecular characterization of thermophilic bacteria with xylanase activity from thermal springs in Erzurum. J Life Sci Technol 3(1):32–35

    Google Scholar 

  40. Turnbull PCB (1996) Bacillus. In: Baron S (ed) Medical microbiology, 4th edn. University of Texas Medical Branch, Galveston

  41. Lee EM, Jeon CO, Choi I, Chang K-S, Kim C-J (2005) Silanimonas lenta gen. nov., sp. nov., a slightly thermophilic and alkaliphilic gammaproteobacterium isolated from a hot spring. Int J Sys Evol Microbiol 55:385–389

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to thank the officials of the Erzurum Technical University High-Technology Applications and Research Center (YUTAM) for their technical support.

Author information

Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey

    Ebru Oztas Gulmus & Arzu Gormez

Authors
  1. Ebru Oztas Gulmus
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Arzu Gormez
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Arzu Gormez.

Ethics declarations

Conflict of interest

All authors declared that there is no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Oztas Gulmus, E., Gormez, A. Identification and Characterization of Novel Thermophilic Bacteria from Hot Springs, Erzurum, Turkey. Curr Microbiol 77, 979–987 (2020). https://doi.org/10.1007/s00284-020-01880-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-020-01880-0

Search

Navigation