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Endocellulase and exocellulase activities of two Streptomyces strains isolated from a forest soil

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Abstract

Two Streptomyces strains, M7a and M23, from a Brazilian forest soil were evaluated for the cellulase production of their superna tants after growth in a microcrystalline cellulose medium, using carboxy methylcellulose and filter paper as substrates at different temperatures and pH values. Endoglucanase and exoglucanase activities were compared to a commercial Trichoderma reesei cellulase using fluorogenic conjugated substrates Similar specific activities were observed for the enzyme preparations of strain M23 and T. reesei. For M7a the activities were about seven times higher than those obtained for T. reesei. Extracellular or cell-associated cellobiase activities were not detected in both strains.

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Authors and Affiliations

  1. Instituto de Microbiologia Prof. Paulo de Góes-UFRJ, CCS, Bloco I, Ilha do Fundão, CEP 21490 021, Rio de Janeiro, Brazil

    Luzia T. A. S. Semêdo, Rosana C. Gomes, Rosangela M. A. Soares, Luiz F. Linhares & Rosalie R. R. Coelho

  2. Instituto de Química-UFRJ, C.T., Bloco A, Ilha do Fundão, CEP 21949 900, Rio de Janeiro, Brazil

    Elba P. S. Bon

Authors
  1. Luzia T. A. S. Semêdo
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  2. Rosana C. Gomes
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  3. Elba P. S. Bon
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  4. Rosangela M. A. Soares
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  5. Luiz F. Linhares
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  6. Rosalie R. R. Coelho
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Correspondence to Rosalie R. R. Coelho.

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Semêdo, L.T.A.S., Gomes, R.C., Bon, E.P.S. et al. Endocellulase and exocellulase activities of two Streptomyces strains isolated from a forest soil. Appl Biochem Biotechnol 84, 267–276 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:267

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  • DOI: https://doi.org/10.1385/ABAB:84-86:1-9:267

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