Abstract
The actinomycete, Micromonospora chalcae produced an extracellular cellulase system when grown on cellulose-containing media. Cellulase activity reached a maximum after 8 days and the β-glucosidase activity was maximal within 16 to 18 days. β-Glucosidase activity was isolated and had a Km of 0.25mM for the substrate p-nitrophenyl-β-D-glucoside. The activity was optimal at pH 7.25 and 52°C.
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Gallagher, J., Winters, A., Barron, N. et al. Production of cellulase and β-glucosidase activity during growth of the actinomycete Micromonospora chalcae on cellulose-containing media. Biotechnol Lett 18, 537–540 (1996). https://doi.org/10.1007/BF00140199
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DOI: https://doi.org/10.1007/BF00140199