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Study of allelic composition of Vrn-1 and Ppd-1 genes in early–ripening and middle–early varieties of spring soft wheat in siberia

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Russian Journal of Genetics: Applied Research

Abstract

Using allele-specific primers, a combination of the Vrn-A1, Vrn-B1, Vrn-D1, Ppd-D1, and Ppd-B1 alleles was analyzed in 48 early-ripening and middle–early varieties of the spring soft wheat from different breeding centers of Siberia. Six haplotypes were detected, including two most widespread with two dominant genes (Vrn-A1 and Vrn-B1) on the background of recessivePpd-D1b gene (causing sensitivity to the photoperiod). The dominant Ppd-D1a allele was found in combination with dominant Vrn-A1 and Vrn-B1 genes only in one variety (Tulun 15), which is the earliest among all the analyzed varieties. A significant variability in the duration of vegetation was detected within each haplotype assuming a strong influence of the genetic background on the given trait. The results obtained can be used in marker-assisted selection of the most optimal genotypes for specific cultivation conditions.

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References

  • Beales, J., Turner, A., Griffiths, S., Snape, J.W., and Laurie, D.A., A pseudo-response regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat (Triticum aestivum L.), Theor. Appl. Genet., 2007, vol. 115, no. 5, pp. 721–733.

    Article  CAS  PubMed  Google Scholar 

  • Distelfeld, A., Li, C., and Dubcovsky, J., Regulation of flowering in temperate cereals, Curr. Opin. Plant Biol., 2009, vol. 12, pp. 1–7.

    Article  Google Scholar 

  • Emtseva, M.V., Efremova, T.T., and Arbuzova, V.S., The influence of Vrn-B1a and Vrn-B1c alleles on the length of developmental phases of substitution and nearisogenic lines of common wheat, Russ. J. Genet., 2013, vol. 49, no. 5, pp. 545–552.

    Article  CAS  Google Scholar 

  • Flyaksberger, K.A., Pshenitsy (Wheats), Moscow: OGIZ, 1938.

    Google Scholar 

  • Fu, D., Szucs, P., Yan, L., Helguera, M., Skinner, J.S., Zitzewits, J.V., Hayes, P.M., and Dubcovsky, J., Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat, Mol. Genet. Genom., 2005, vol. 273, pp. 54–65.

    Article  CAS  Google Scholar 

  • Gomeza, D., Vanzettia, L., Helgueraa, M., Lombardoa, L., Fraschinaa, J., and Miralles, D.J., Effect of Vrn-1, Ppd-1 genes and earliness per se on heading time in Argentinean bread wheat cultivars, Field Crops Res., 2014, vol. 158, pp. 73–81.

    Article  Google Scholar 

  • Iqbal, M., Shahzad, A., and Ahmed, I., Allelic variation at the Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3 and Ppd-D1a loci of Pakistani spring wheat cultivars, Electron. J. Biotechnol., 2010, vol. 14, pp. 1–8.

    Google Scholar 

  • Kosner, J. and Pankova, K., Chromosome substitutions with dominant loci Vrn-1 and their effect on developmental stages of wheat, Czech J. Genet. Plant Breed., 2004, vol. 40, no. 2, pp. 37–44.

    Google Scholar 

  • Kumakov, V.A., Fiziologiya yarovoi pshenitsy (Physiology of Spring Wheat), Moscow: Kolos, 1980.

    Google Scholar 

  • Kumar, S., Sharma, V., Chaudhary, S., Tyagi, A., Mishra, P., Priyadarshini, A., and Singh, A., Genetics of flowering time in bread wheat Triticum aestivum: complementary interaction between vernalization-insensitive and photoperiod-insensitive mutations imparts very early flowering habit to spring wheat, J. Genet., 2012, vol. 91, no. 1, pp. 33–47.

    Article  PubMed  Google Scholar 

  • Leont’ev, S.I., Osnovnye parametry modelei sortov yarovoi pshenitsy intensivnogo tipa dlya stepi i yuzhnoi lesostepi Zapadnoi Sibiri (The Main Parameters of the Models of Spring Wheat Cultivars of Intensive Type for the Steppe and Southern Forest-Steppe of West Siberia), Omsk: OmSKhI, 1980.

    Google Scholar 

  • Merezhko, A.F., Popolnenie, sokhranenie v zhivom vide i izuchenie mirovoi kollektsii pshenitsy, egilopsa i triticale. Metodicheskie ukazaniya (Replenishment, preservation in a living form, and study of the world collection of wheat, Aegilops, and triticale: Guidelines), St. Petersburg: VIR, 1999.

    Google Scholar 

  • Moiseeva, E.A. and Goncharov, N.P., Genetic control of the spring habit in old local cultivars and landraces of common wheat from Siberia, Russ. J. Genet., 2007, vol. 43, no. 4, pp. 369–375.

    Article  CAS  Google Scholar 

  • Nishida, H., Yoshida, T., Kawakami, K., et al., Structural variation in the 5’upstream region of photoperiod insensitive alleles Ppd-A1a and Ppd-B1a identified in hexaploid wheat (Triticum aestivum L.), and their effect on heading time, Mol. Breeding, 2013, vol. 31, no. 1, pp. 27–37.

    Article  CAS  Google Scholar 

  • Potokina, E.K., Koshkin, V.A., Alekseeva, E.A., Matvienko, I.I., Filobok, V.A., and Bespalova, L.A., The combination of the Ppd and Vrn gene alleles determines the heading date in common wheat varieties, Russ. J. Genet.: Appl. Res., 2012, vol. 2, no. 4, pp. 311–318.

    Article  Google Scholar 

  • Rudenko, M.I., The problem of selective use of durum wheat in the South-East regions, Tr. Prikl. Botan. Genet. Sel., 1960, vol. 32, no. 2, pp. 227–247.

    Google Scholar 

  • Scarth, R. and Law, C.N., The control of day length response in wheat by the group 2 chromosomes, Z. Pflanzenzgcht, 1984, vol. 92, pp. 140–150.

    Google Scholar 

  • Shcherban, A.B., Efremova, T.T., and Salina, E.A., Identification of a new Vrn-B1 allele using two near-isogenic wheat lines with difference in heading time, Mol. Breeding, 2012a, vol. 29, no. 3, pp. 675–685.

    Article  CAS  Google Scholar 

  • Shcherban, A.B., Emtseva, M.V., and Efremova, T.T., Molecular genetical characterization of vernalization genes Vrn-A1, Vrn-B1 and Vrn-D1 in spring wheat germplasm from Russia and adjacent regions, Cereal Res. Commun., 2012b, vol. 40, pp. 54–65.

    Article  Google Scholar 

  • Shcherban, A.B., Boerner, A., and Salina, E.A., Effect of VRN-1 and PPD-D1 genes on heading time in European bread wheat cultivars, Plant Breed., 2014. doi:10.1111/pbr.12223

    Google Scholar 

  • Stelmakh, A.F., Izuchenie genetiki tipa i skorosti razvitiya myagkikh pshenits vo VSGI, Genetiko-Tsitologicheskie aspekty selektsii sel’skokhozyaistvennykh rastenii: Cb. nauch. tr. (The Study of Genetics of the Type and Growth Rate Speed of Common Wheat at VSGI: Col- lected Scientific Papers), Odessa, 1984, pp. 5–15.

    Google Scholar 

  • Stelmakh, A.F., The genetic nature of the typical alternate wheat, S.-Kh. Biol., 1986, no. 2, pp. 22–30.

    Google Scholar 

  • Stelmakh, A.F., The role of genetic systems in the ontogenetic adaptation of common wheat, in Ekologicheskaya genetika i evolyutsiya (Ecological Genetics and Evolution), Chisinau, 1987, pp. 146–161.

    Google Scholar 

  • Stelmakh, A.F., Geographic distribution of VRN genes in landraces and improved varieties of spring bread wheat, Euphytica, 1990, vol. 45, pp. 113–118.

    Google Scholar 

  • Stelmakh, A.F., Genetic effects of VRN genes on heading date and agronomic traits in bread wheat, Euphytica, 1993, vol. 65, pp. 53–60.

    Article  Google Scholar 

  • Vavilov, N.I., Botanical and geographical bases of breeding, in Teoreticheskie osnovy selektsii rastenii (Theoretical Bases of Plant Breeding), Moscow: Sel’khozgiz, 1935, vol. 1, pp. 17–94.

    Google Scholar 

  • Voronin, A.N. and Stel’makh, A.F., Stages of organogenesis in common wheat lines nearly isogenic for Vrn1–3 loci, Nauch.-Tekhn. byul. VSGI, 1985, no. 1 (55), pp. 19–23.

    Google Scholar 

  • Worland, A.J., The influence of flowering time genes on environmental adaptability in European wheats, Euphytica, 1996, vol. 89, pp. 49–57.

    Article  Google Scholar 

  • Worland, A.J., Borner, A., Korzun, V., Li, M.W., Petrovic, S., and Sayers, E.J., The influence of photoperiod genes on the adaptability of European winter wheat, Euphytica, 1998, vol. 100, pp. 385–394.

    Article  CAS  Google Scholar 

  • Yan, L., Helguera, M., Kato, K., Fukuyama, S., Sherman, J., and Dubcovsky, J., Allelic variation at the VRN-1 promoter region in polyploid wheat, Theor. Appl. Genet., 2004, vol. 109, pp. 1677–1686.

    Article  CAS  PubMed  Google Scholar 

  • Zykin, V.A., Shamanin, V.P., and Belan, I.A., Ekologiya pshenitsy (Wheat Ecology), Omsk: Izd. FGOU VPO OmGAU, 2000.

    Google Scholar 

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Correspondence to E. A. Salina.

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Original Russian Text © I.E. Likhenko, A.I. Stasyuk, A.B. Shcherban’, A.F. Zyryanova, N.I. Likhenko, E.A. Salina, 2014, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2014, Vol. 18, No. 4/1, pp. 691–703.

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Likhenko, I.E., Stasyuk, A.I., Shcherban’, A.B. et al. Study of allelic composition of Vrn-1 and Ppd-1 genes in early–ripening and middle–early varieties of spring soft wheat in siberia. Russ J Genet Appl Res 5, 198–207 (2015). https://doi.org/10.1134/S2079059715030107

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  • DOI: https://doi.org/10.1134/S2079059715030107

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