Skip to main content
SpringerLink
Log in

Freezing of dehydrated calli of spring wheat (Triticum aestivum L.) in liquid nitrogen and their morphogenetic potential

  • Botany
  • Published:
Biology Bulletin Aims and scope Submit manuscript

Abstract

A cryopreservation method developed earlier was modified for freezing of calli derived from mature embryos of four spring wheat (Triticum aestivum L.) pure lines. The effects of particular stages of cryopreservation protocol on water content, number of calli recovering growth, and rate of morphogenesis were analyzed. Regrowth was observed in 90.5–100% of calli after dehydration and in 93.3–100% after freezing-thawing. Dehydration, but not freeze-thawing significantly decreased the frequency of morphogenetic variation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bajaj, Y.P.S., Freeze-Preservation of Plant Cells—a Novel Approach to the Conservation of Gemplasm, in Genetics and Wheat Improvement, Gupta, A.K., Ed., Delhi: Oxford, IBH, 1980, pp. 141–149.

    Google Scholar 

  • Bajaj, Y.P.S., Survival of Somatic Hybrid Protoplasts of Wheat × Pea and Rice × Pea Subjected To −196°C, Indian J. Exp. Biol., 1983, vol. 21, pp. 120–122.

    Google Scholar 

  • Blakesley, D., Mazrooei, S.A., Bhatti, M.H., and Henshaw, G.G., Cryopreservation of Non-Encapsulated Embryogenic Tissue of Sweet Potato (Ipomoea batatas), Plant Cell Rep., 1996, vol. 15, pp. 873–876.

    Article  CAS  Google Scholar 

  • Butenko, R.G., Popov, A.S., Volkova, L.A., et al., Recovery of Cell Cultures and Their Biosynthetic Capacity after Storage of Dioscorea deltoidea and Panax ginseng Cells in Liquid Nitrogen, Plant Sci. Lett., 1984, vol. 33, pp. 285–292.

    Article  CAS  Google Scholar 

  • Butenko, R.G., Popov, A.S., Volkova, L.A., et al., Plant Cell Viability at Different Deep Freezing Protocols, Tsitologiya, 1983, no. 10, pp. 1191–1196.

  • Chaturvedi, H.C. and Mitra, G.C., A Shift in Morphogenetic Pattern in Citrus Callus Tissue during Prolonged Culture, Ann. Botany, 1975, vol. 39, pp. 683–687.

    Google Scholar 

  • Chen, T.H.H., Kartha, K.K., and Gusta, L.V., Cryopreservation of Wheat Suspension Culture and Regenerable Callus, Plant Cell Tiss. Organ Cult., 1985, vol. 4, pp. 101–109.

    Article  Google Scholar 

  • Cho, J.S., Hong, S.M., Joo, S.Y., et al., Cryopreservation of Transgenic Rice Suspension Cells Producing Recombinant HCTLA4Ig, Applied Microbiol. Biotechnol., 2007, vol. 73, no. 6, pp. 1470–1476.

    Article  CAS  Google Scholar 

  • Danso, K.E. and Ford-Lloyd, B.V., Cryopreservation of Embryogenic Calli of Cassava using Sucrose Cryoprotection and Air Desiccation, Plant Cell Rep., 2004, vol. 22, pp. 623–631.

    Article  CAS  PubMed  Google Scholar 

  • Dumet, D., Engelmann, F., Chabrillange, N., and Duval, Y., Cryopreservation of Oil Palm (Elaeis guineensis Jacq.) Somatic Embryos Involving a Desiccation Step, Plant Cell Rep., 1993, vol. 12, pp. 352–355.

    Article  CAS  Google Scholar 

  • Engelmann, F., Plant Cryopreservation: Progress and Prospects, In Vitro Cell. Dev. Biol. Plant., 2004, vol. 40, no. 5, pp. 427–433.

    Article  Google Scholar 

  • Karp, A., Somaclonal Variation As a Tool for Crop Improvement, Euphytica, 1995, vol. 85, pp. 295–302.

    Article  Google Scholar 

  • Keefe, P. and Henshaw, G., A Note on the Multiple Role of Artificial Nucleation of the Suspending Medium during Two-Step Cryopreservation Procedures, Cryoletters, 1984, vol. 5, pp. 71–78.

    Google Scholar 

  • Kendall, E.J., Qureshi, J.A., Kartha, K.K., et al., Regeneration of Freezing-Tolerant Spring Wheat (Triticum aestivum, L.) Plants from Cryoselected Callus, Plant Physiol., 1990, vol. 94, pp. 1756–1762.

    Article  CAS  PubMed  Google Scholar 

  • Linch, P.T., Benson, E.E., Jones, J., et al., The Embryogenic Potential of Rice Cell Suspensions Affects Their Recovery Following Cryogenic Storage, Euphytica, 1995, vol. 85, pp. 347–349.

    Article  Google Scholar 

  • Matsubayashi, Y. and Sakagami, Y., Phytosulfokine, Sulfated Peptides That Induce the Proliferation of Single Mesophyll Cells of Asparagus officinalis, L., Proc. Natl. Acad. Sci. USA. Cell Biology, 1996, vol. 93, pp. 7623–7627.

    Article  CAS  Google Scholar 

  • Mazur, P., Cryobiology: The Freezing of Biological System, Science, 1970, vol. 168, no. 3934, pp. 939–949.

    Article  CAS  PubMed  Google Scholar 

  • Moukadiri, O., Lopes, C.R., and Cornejo, M.J., Physiological and Genomic Variations in Rice Cells Recovered from Direct Immersion and Storage in Liquid Nitrogen, Physiol. Plant., 1999, vol. 105, pp. 442–449.

    Article  CAS  Google Scholar 

  • Murashige, T. and Skoog, F., A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures, Physiol. Plant., 1962, vol. 15, no. 4, pp. 473–497.

    Article  CAS  Google Scholar 

  • Panis, B. and Lambardi, M., Status of Cryopreservation Technologies in Crops and Forest, in The Role of Biotechnology, Villa Gualino, Turin, Italy, March 5–7, 2005, pp. 43–54.

  • Plokhinskii, N.A., Matematicheskie Metody v Biologii (Mathematical Methods in Biology), Moscow: Mosk. Gos. Univ., 1978.

    Google Scholar 

  • Popov, A.S. and Fedorovskii, D.N., Lesions of Plasmalemma of Diascorea Cells Cultured in vitro during Their Cryopreservation, Fiziol. Rast., 1992, vol. 39, no. 2, pp. 335–343.

    Google Scholar 

  • Popov, A.S., Chernyak, N.D., and Paukov, V.N., Improvement of Reculturing of Cells after Their Storage in Liquid Nitrogen, Fiziol. Rast., 1984, vol. 31, no. 3, pp. 602–605.

    CAS  Google Scholar 

  • Popov, A.S., Some Mechanisms of Plant Cell Cryodamage in vitro and Characteristic of Their Cryopreservation, Fiziol. Rast., 1993, vol. 40, no. 3, pp. 485–496.

    Google Scholar 

  • Popova, E.V., Lee, E.J., Wu, C.H., et al., A Simple Method for Cryopreservation of Ginkgo Biloba Callus, Plant Cell Tiss. Organ Cult., 2009, vol. 97, pp. 337–343.

    Article  CAS  Google Scholar 

  • Pritchard, H.W., Grout, B.W.W., and Short, K.C., Osmotic Stress as a Pregrowth Procedure for Cryopreservation 1. Growth and Ultrastructure of Sycamore and Soybean Cell Suspensions, Ann. Botany, 1986, vol. 57, pp. 41–48.

    Google Scholar 

  • Vysotskaya, O.N., Danilova, S.A., and Popov, A.S., Method of Cryopreservation in vitro of Meristems Isolated from Strawberry, RF Patent No. 2 302 107, Byull. Izobret., no. 19, 2007.

  • Salcheva, G. and Samygin, G.A., Microscopic observations of Freezing of Winter Wheat Tissues, Fiziol. Rast., 1963, vol. 10, no. 1, pp. 65–72.

    Google Scholar 

  • Samygin, G.A., Prichiny vymerzaniya rastenii (Causes of Plant Winterkilling), Moscow: Nauka, 1974.

    Google Scholar 

  • Samygin, G.A., Causes of Damage of Plant Cells by Extracellular Ice, Fiziol. Rast., 1994, vol. 41, pp. 614–625.

    Google Scholar 

  • Sowa, S., Oleszczuk, S., and Zimny, J., A Simple and Efficient Method for Cryopreservation of Embryogenic Triticale Calli, ACTA Physiol. Plant, 2005, vol. 27, no. 2, pp. 237–243.

    Article  Google Scholar 

  • Steponkus, P., Dowgert, M., and Gordon-Kamm, W., Destabilization of the Plasma Membrane of Isolated Plant Protoplasts during a Freeze-Thaw Cycle: The Influence of Cold Acclimation, Cryobiology, 1983, vol. 20, no. 4, pp. 448–465.

    Article  CAS  PubMed  Google Scholar 

  • Tantau, H. and Darffling, K., In vitro-Selection of Hydroxyproline-Resistant Cell Lines of Wheat (Triticum aestivum): Accumulation of Proline, Decrease in Osmotic Potential, and Increase in Frost Tolerance, Physiol. Plant., 1991, vol. 82, no. 2, pp. 243–248.

    Article  CAS  Google Scholar 

  • Trunova, T.I., Sugars as a Factor Increases Frost Hardiness of Plants, Izv. Akad. Nauk SSSR, Ser. Biol., 1972, no. 2, pp. 185–189.

  • Tumanov, I.I., Krasavtsev, O.A., and Trunova, T.I., Survival of Winter Wheat at −196°C as a Result of Vitrification, Dokl. Akad. Nauk SSSR, 1965, vol. 161, pp. 978–981.

    Google Scholar 

  • Vannini, G.L. and Poli, F., Binucleation and Abnormal Chromosome Distribution in Euglena gracilis Cells Treated with Dimethyl Sulfoxide, Protoplasma, 1983, vol. 114, pp. 62–66.

    Article  CAS  Google Scholar 

  • Wang, W.C., Shang, X.M., Ycel, M., and Nguyen, H.T., Selection of Cultured Wheat Cells for Tolerance to High Temperature Stress, Crop Sci. Soc. Amer., 1993, vol. 33, pp. 315–320.

    Article  Google Scholar 

  • Wang, X.J., Loh, C.S., Yeoh, H.H., and Sun, W.Q., Differential Mechanisms to Induce Dehydration Tolerance by Abscisic Acid and Sucrose in Spathoglottis plicata (Orchidaceae) Protocorms, Plant Cell Environ., 2003, vol. 26, pp. 737–744.

    Article  CAS  Google Scholar 

  • Zhang, L., Rybczynski, J.J., Langenberg, W.G., et al., An Efficient Wheat Transformation Procedure: Transformed Calli with Long-Term Morphogenic Potential for Plant Regeneration, Plant Cell Rep., 2000, vol. 19, pp. 241–250.

    Article  CAS  Google Scholar 

  • Zhang, Y.X., Wang, J.H., Bian, H.W., and Zhu, M.Y., Pregrowth-Desiccation: A Simple and Efficient Procedure for the Cryopreservation of Rice (Oryza sativa (L.) Embryogenic Suspension Cells, Cryoletters, 2001, vol. 22, no. 4, pp. 221–228.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, 127276, Russia

    A. I. Solov’eva, O. N. Vysotskaya, A. S. Popov & Yu. I. Dolgikh

Authors
  1. A. I. Solov’eva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. N. Vysotskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. S. Popov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yu. I. Dolgikh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. I. Solov’eva.

Additional information

Original Russian Text © A.I. Solov’eva, O.N. Vysotskaya, A.S. Popov, Yu.I. Dolgikh, 2010, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2010, No. 5, pp. 574–580.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Solov’eva, A.I., Vysotskaya, O.N., Popov, A.S. et al. Freezing of dehydrated calli of spring wheat (Triticum aestivum L.) in liquid nitrogen and their morphogenetic potential. Biol Bull Russ Acad Sci 37, 489–495 (2010). https://doi.org/10.1134/S1062359010050080

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1062359010050080

Keywords

Search

Navigation