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Molecular characterization of MYB transcription factor genes from Panax ginseng

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Abstract

Transcription factors (TFs) are essential for gene regulation in all living organisms, including plants, where among numerous other functions, they control temporal and spatial gene expression in response to environmental stimuli. The v-myb avian myeloblastosis viral oncogene homolog (MYB) family is the largest TF family in plants, with its members involved in developmental processes as well as secondary metabolism. Little is known about MYB genes in Panax ginseng Meyer, despite ginseng’s importance as a widely-used medicinal plant. In this study, we isolated nine MYB genes from P. ginseng (PgMYBs). Phylogenetic comparison of these genes with the MYB genes of other plant species revealed that the PgMYBs clustered into different families based on their putative functions including terpene regulation; five PgMYBs clustered in the R2R3 family, and four PgMYBs clustered in the MYB-related protein group. Further expression analysis of five PgMYBs showed consistently high expression in flower and leaf tissue, suggesting that these PgMYB genes may be involved in development of the above-mentioned tissues. Four PgMYBs were downregulated in response to methyl jasmonate (MJ) and salicylic acid (SA), whereas PgMYB3 was up-regulated, suggesting a role for all these genes in stress response. This is the first comprehensive study of the MYB gene family in P. ginseng, and the information provided here will facilitate further exploration of the functions of these TFs.

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Abbreviations

ESTs:

expressing sequence tags

JA:

jasmonic acid

MJ:

methyl jasmonate

MYB:

myeloblastosis viral oncogene homolog

SA:

salicylic acid

TFs:

transcription factors

References

  1. Mitsuda, N. and Ohme-Takagi, M., Functional analysis of transcription factors in Arabidopsis, Plant Cell Physiol., 2009, vol. 50, pp. 1232–1248.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Dubos, C., Stracke, R., Grotewold, E., Weisshaar, B., Martin, C., and Lepiniec, L., MYB transcription factors in Arabidopsis, Trends Plant Sci., 2010, vol. 15, pp. 573–581.

    Article  CAS  PubMed  Google Scholar 

  3. Ogata, K., Structure and dynamics of the transcription factor, Myb, in DNA-sequence recognition, Seikagaku, 1998, vol. 70, pp. 1233–1250.

    CAS  PubMed  Google Scholar 

  4. Wang, F., Suo, Y., Wei, H., Li, M., Xie, C., Wang, L., Chen, X., and Zhang, Z., Identification and characterization of 40 isolated Rehmannia glutinosa MYB family genes and their expression profiles in response to shading and continuous cropping, Int. J. Mol. Sci., 2015, vol. 16, pp. 15 009–15 030.

    CAS  Google Scholar 

  5. Kim, Y.J., Zhang, D., and Yang, D.C., Biosynthesis and biotechnological production of ginsenosides, Biotechnol. Adv., 2015, vol. 33, pp. 717–735.

    Article  CAS  PubMed  Google Scholar 

  6. Rahimi, S., Kim, Y.J., and Yang, D.C., Production of ginseng saponins: elicitation strategy and signal transductions, Appl. Microbiol. Biotechnol., 2015, vol. 99, pp. 6987–6996.

    Article  CAS  PubMed  Google Scholar 

  7. Afrin, S., Zhu, J., Cao, H., Huang, J., Xiu, H., Luo, T., and Luo, Z., Molecular cloning and expression profile of an abiotic stress and hormone responsive MYB transcription factor gene from Panax ginseng, Acta Biochim. Biophys. Sin., 2015, vol. 47, pp. 267–277.

    Article  PubMed  Google Scholar 

  8. Kim, M.K., Lee, B.S., In, J.G., Sun, H., Yoon, J.H., and Yang, D.C., Comparative analysis of expressed sequence tags (ESTs) of ginseng leaf, Plant Cell Rep., 2006, vol. 25, pp. 599–606.

    Article  CAS  PubMed  Google Scholar 

  9. Kyte, J. and Doolittle, R.F., A simple method for displaying the hydropathic character of a protein, J. Mol. Biol., 1982, vol. 157, pp. 105–132.

    Article  CAS  PubMed  Google Scholar 

  10. Howarth, D.G. and Donoghue, M.J., Duplications and expression of DIVARICATA-like genes in Dipsacales, Mol. Biol. Evol., 2009, vol. 26, pp. 1245–1258.

    Article  CAS  PubMed  Google Scholar 

  11. Subramaniyam, S., Mathiyalagan, R., Natarajan, S., Kim, Y.J., Jang, M.G., Park, J.H., and Yang, D.C., Transcript expression profiling for adventitious roots of Panax ginseng Meyer, Gene, 2014, vol. 546, pp. 89–96.

    Article  CAS  PubMed  Google Scholar 

  12. Lipsick, J.S., One billion years of Myb, Oncogene, 1996, vol. 18, pp. 223–235.

    Google Scholar 

  13. Bonke, M., Thitamadee, S., Mahonen, A.P., Hauser, M.T., and Yka, H., APL regulates vascular tissue identity in Arabidopsis, Nature, 2003, vol. 426, pp. 181–186.

    Article  CAS  PubMed  Google Scholar 

  14. Meneses, E., Cárdenas, H., Zárate, S., Brieba, L.G., Orozco, E., López-Camarillo, C., and Azuara-Liceaga, E., The R2R3 Myb protein family in Entamoeba histolytica, Gene, 2010, vol. 455, pp. 32–42.

    Article  CAS  PubMed  Google Scholar 

  15. Zoulias, N., Koenig, D., Hamidi, A., McCormick, S., and Kim, M., A role for PHANTASTICA in medio-lateral regulation of adaxial domain development in tomato and tobacco leaves, Ann. Bot., 2012, vol. 109, pp. 407–418.

    Article  CAS  PubMed  Google Scholar 

  16. Laitinen, R.A.E., Ainasoja, M., Broholm, S.K., Teeri, T.H., and Elomaa, P., Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida, J. Exp. Bot., 2008, vol. 59, pp. 3692–3703.

    Article  Google Scholar 

  17. Zhao, Q. and Dixon, R.A., Transcriptional networks for lignin biosynthesis: more complex than we thought? Trends Plant Sci., 2011, vol. 16, pp. 227–233.

    Article  CAS  PubMed  Google Scholar 

  18. Jung, C., Seo, J.S., Han, S.W., Koo, Y.J., Kim, C.H., Song, S.I., Nahm, B.H., Choi, Y.D., and Cheong, J.J., Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis, Plant Physiol., 2008, vol. 146, pp. 623–635.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Shan, H., Chen, S., Jiang, J., Chen, F., Chen, Y., Gu, C., Li, P., Song, A., Zhu, X., Gao, H., Zhou, G., Li, T., and Yang, X., Heterologous expression of the chrysanthemum R2R3-MYB transcription factor CmMYB2 enhances drought and salinity tolerance, increases hypersensitivity to ABA and delays flowering in Arabidopsis thaliana, Mol. Biotechnol., 2012, vol. 51, pp. 160–173.

    Article  CAS  PubMed  Google Scholar 

  20. Liu, J., Ishitani, M., Halfter, U., Kim, C.S., and Zhu, J.K., The Arabidopsis thaliana SOS2 gene encodes a protein kinase that is required for salt tolerance, Proc. Natl. Acad. Sci. USA, 2000, vol. 97, pp. 3730–3734.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Jin, H. and Martin, C., Multifunctionality and diversity within the plant MYB-gene family, Plant Mol. Biol., 1999, vol. 41, pp. 577–585.

    Article  CAS  PubMed  Google Scholar 

  22. Sehr, E.M., Agusti, J., Lehner, R., Farmer, E.E., Schwarz, M., and Greb, T., Analysis of secondary growth in the Arabidopsis shoot reveals a positive role of jasmonate signalling in cambium formation, Plant J., 2010, vol. 63, pp. 811–822.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Vom Endt, D., Soares e Silva, M., Kijne, J.W., Pasquali, G., and Memelink, J., Identification of a bipartite jasmonate-responsive promoter element in the Catharanthus roseus ORCA3 transcription factor gene that interacts specifically with AT-hook DNA-binding proteins, Plant Physiol., 2007, vol. 144, pp. 1680–1690.

    Article  Google Scholar 

  24. Yan, H., Zhang, H., Wang, Q., Jian, H., Qiu, X., Wang, J., and Tang, K., Isolation and identification of a putative scent-related gene RhMYB1 from rose, Mol. Biol. Rep., 2011, vol. 38, pp. 4475–4482.

    Article  CAS  PubMed  Google Scholar 

  25. Höll, J., Vannozzi, A., Czemmel, S., D’Onofrio, C., Walker, A.R., Rausch, T., Lucchin, M., Boss, P.K., Dry, I.B., and Bogs, J., The R2R3-MYB transcription factors MYB14 and MYB15 regulate stilbene biosynthesis in Vitis vinifera, Plant Cell, 2013, vol. 25, pp. 4135–4149.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Liu, Y., Shi, Z., Maximova, S.N., Payne, M.J., and Guiltinan, M.J., Tc-MYBPA an Arabidopsis TT2-like transcription factor and functions in the regulation of proanthocyanidin synthesis in Theobroma cacao, BMC Plant Biol., 2015, vol. 15, p. 160.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Kobayashi, S., Ishimaru, M., Hiraoka, K., and Honda, C., Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis, Planta, 2002, vol. 215, pp. 924–933.

    Article  CAS  PubMed  Google Scholar 

  28. An, X.H., Tian, Y., Chen, K.Q., Liu, X.J., Liu, D.D., Xie, X.B., Cheng, C.G., Cong, P.H., and Hao, Y.J., MdMYB9 and MdMYB11 are involved in the regulation of the JA-induced biosynthesis of anthocyanin and proanthocyanidin in apples, Plant Cell Physiol., 2015, vol. 56, pp. 650–662.

    Article  CAS  PubMed  Google Scholar 

  29. Mahjoub, A., Hernould, M., Joubès, J., Decendit, A., Mars, M., Barrieu, F., Hamdi, S., and Delrot, S., Overexpression of a grapevine R2R3-MYB factor in tomato affects vegetative development, flower morphology and flavonoid and terpenoid metabolism, Plant Physiol. Biochem., 2009, vol. 47, pp. 551–561.

    Article  CAS  PubMed  Google Scholar 

  30. Song, S., Qi, T., Huang, H., Ren, Q., Wu, D., Chang, C., Peng, W., Liu, Y., Peng, J., and Xie, D., The Jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription factors MYB21 and MYB24 to affect jasmonate-regulated stamen development in Arabidopsis, Plant Cell, 2011, vol. 23, pp. 1000–1013.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin, 446-701, Korea

    J. Y. Choi, Y. J. Kim, I. S. Chung, W. S. Kwon & D. C. Yang

  2. Graduate School of Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Korea

    R. Abbai, J. Silva, S. Rahimi, D. Myagmarjav & D. C. Yang

Authors
  1. J. Y. Choi
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  2. R. Abbai
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  3. Y. J. Kim
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  4. J. Silva
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  5. S. Rahimi
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  6. D. Myagmarjav
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  7. I. S. Chung
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  8. W. S. Kwon
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  9. D. C. Yang
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Correspondence to J. Y. Choi or R. Abbai.

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These authors contributed equally to this work.

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Choi, J.Y., Abbai, R., Kim, Y.J. et al. Molecular characterization of MYB transcription factor genes from Panax ginseng . Russ J Plant Physiol 64, 398–409 (2017). https://doi.org/10.1134/S1021443717030050

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