Abstract
The expressed sequence tags (ESTs) referenced in this report are the first transcriptomes in a leaf from a half-shade ginseng plant. A cDNA library was constructed from samples of the leaves of 4-year-old Panax ginseng plants, which were cultured in a field. The 2,896 P. ginseng cDNA clones represent 1,576 unique sequences, consisting of 1,167 singletons and 409 contig sequences. BLAST comparisons of the cDNAs in GenBank's non-redundant databases revealed that 2,579 of the 2,896 cDNAs (89.1%) exhibited a high degree of sequence homology to genes from other organisms. The majority of the identified transcripts were found to be genes related with energy, metabolism, subcellular localization, and protein synthesis and transport. The chlorophyll a/b-binding protein ESTs in the ginseng leaf samples manifested a substantially higher level of expression than was observed in other plant leaves. The ESTs involved in ginsenoside biosynthesis were also identified and discussed.
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Abbreviations
- CAB:
-
chlorophyll a/b-binding protein
- cDNA:
-
complementary DNA
- ESTs:
-
expressed sequence tags
References
Ablett E, Seaton G, Scott K, Shelton D, Graham MW, Baverstock P, Lee LS, Henry R (2000) Analysis of grape ESTs: Global gene expression patterns in leaf and berry. Plant Sci 159:87–95
Bishop GJ, Nomura T, Yokota T, Harrison K, Noguchi T, Fujioka S, Takatsuto S, Jones JDG, Kamiya Y (1999) The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis. Proc Natl Acad Sci USA 96:1761–1766
Bohlmann J, Meyer-Gauen G, Croteau R (1998) Plant terpenoid synthases: Molecular biology and phylogenetic analysis. Proc Natl Acad Sci USA 95:4126–4133.
Brandle JE, Richman A, Swanson AK, Chapman BP (2002) Leaf ESTs from Stevia rebaudiana: A resource for gene discovery in diterpene synthesis. Plant Mol Biol 50:613–622
Choi DW, Jung JD, Ha YI, Park HW, In DS, Chung HJ, Liu JR (2005) Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites. Plant Cell Rep 23:557–566
De la Torre WR, Burkey KO (1991) Acclimation of barley to changes in light intensity: Photosynthetic electron transport activity and components. Photosynth Res 24:127–136
Devarenne TP, Ghosh A, Chappell J (2002) Regulation of squalene synthase, a key enzyme of sterol biosynthesis, in tobacco. Plant Physiol 129:1095–1106
Green BR, Salter AH (1996) Light regulation of nuclear-encoded thylakoid proteins. In: Andersson B, Salter AH, Barber J (eds) Molecular Genetics of Photosynthesis. Oxford University Press, Oxford, pp 75–103
Haralampidis K, Bryan G, Qi X, Papadopoulou K, Bakht S, Melton R, Osbourn A (2001) A new class of oxidosqualene cyclases directs synthesis of antimicrobial phytoprotectants in monocots. Proc Natl Acad Sci USA 98:13431–13436
Haralampidis K, Trojanowska M, Osbourn AE (2002) Biosynthesis of triterpenoid saponins in plants. Adv Biochem Eng Biotechnol 75:31–49
Hayashi H, Huang P, Kirakosyan A, Inoue K, Hiraoka N, Ikeshiro Y, Kushiro T, Shibuya M, Ebizuka Y (2001) Cloning and characterization of a cDNA encoding beta-amyrin synthase involved in glycyrrhizin and soyasaponin biosyntheses in licorice. Biol Pharm Bull 24:912–916
Jung JD, Park HW, Hahn Y, Hur CG, In DS, Chung HJ, Liu JR, Choi DW (2003) Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tags. Plant Cell Rep 22:224–230
Kushiro T, Shibuya M, Ebizuka Y (1998) Beta-amyrin synthase: Cloning of oxidosqualene cyclase that catalyzes the formation of the most popular triterpene among higher plants. Eur J Biochem 256:238–244
Morris PC, Kumar A, Bowles DJ, Cuming AC (1990) Osmotic stress and abscisic acid regulate the expression of the Em gene of wheat. Eur J Biochem 190:625–630
Morita M, Shibuya M, Kushiro T, Masuda K, Ebizuka Y (2000) Molecular cloning and functional expression of triterpene synthases from pea (Pisum sativum). Eur J Biochem 267:3453–3460
Seemann JR, Sharkey TD, Wang J, Osmond CB (1987) Environmental effects on photosynthesis, nitrogen use efficiency and metabolite pools in leaves of sun and shade plants. Plant Physiol 84:796–802
Sticher O (1998) Getting to the root of ginseng. Chemtech 28:26–32
Stitt M (1986) Limitation of photosynthesis by carbon metabolism I. Evidence for excess electron transport capacity in leaves carrying out photosynthesis in saturating light and CO2. Plant Physiol 81:1115–1122
Suzuki H, Achnine L, Xu R, Matsuda SP, Dixon RA (2002) A genomics approach to the early stages of triterpene saponin biosynthesis in Medicago truncatula. Plant J 32:1033–1048
Vogler BK, Pittler MH, Ernst E (1999) The efficacy of ginseng, a systematic review of randomized clinical trials. Eur J Clin Pharmacol 55:567–575.
Yang W, Bai X, Kabelka E, Eaton C, Kamoun S, van der Knaap E, Francis D (2004) Discovery of single nucleotide polymorphisms in Lycopersicon esculentum by computer aided analysis of expressed sequence tags. Mol Breeding 14:21–34
Walters RG, Horton P (1994) Acclimation of Arabidopsis thaliana to the light environment: Changes in composition of the photosynthetic apparatus. Planta 195:248–256
White JA, Todd J, Newman T, Focks N, Girke T, Martínez de Ilárduya M, Jaworski JG, Ohlrogge JB, Benning C (2000) A new set of Arabidopsis expressed sequence tags from developing seeds. The metabolic pathway from carbohydrates to seed oil. Plant Physiol 124:1582–1594
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This work was financially supported by the Rural Development Administration, BIOGREEN 21 Project.
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Communicated by I. S. Chung
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Kim, M.K., Lee, BS., In, JG. et al. Comparative analysis of expressed sequence tags (ESTs) of ginseng leaf. Plant Cell Rep 25, 599–606 (2006). https://doi.org/10.1007/s00299-005-0095-0
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DOI: https://doi.org/10.1007/s00299-005-0095-0