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Heterologous Expression of the Chrysanthemum R2R3-MYB Transcription Factor CmMYB2 Enhances Drought and Salinity Tolerance, Increases Hypersensitivity to ABA and Delays Flowering in Arabidopsis thaliana

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Abstract

Knowledge on genes related to plant responses to adverse growth conditions and development is essential for germplasm improvement. In this study, a chrysanthemum R2R3-MYB transcription factor gene, designated CmMYB2 (GenBank accession No. JF795918), was cloned and functionally characterized. Expression of CmMYB2 in chrysanthemum leaves was up-regulated in response to drought, salinity and cold stress, as well as by treatment with exogenous abscisic acid (ABA). When the gene was constitutively expressed in Arabidopsis thaliana, it increased plant sensitivity to ABA and reduced stomatal aperture. Plant survival under drought was improved than in the wild type, as was the plants’ salinity tolerance. The level of expression of a number of genes associated with the stress response, including RD22, RD29A, RAB18, COR47, ABA1 and ABA2, was raised in the CmMYB2 transgenic Arabidopsis plants. CmMYB2 transgenic Arabidopsis plants were also delayed in flowering. The expression of CONSTANS (CO), FLOWERING LOCUS T (FT), SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), LEAFY (LFY) and APETALA1 (AP1) genes involved in flowering was down-regulated in the CmMYB2 transgenics. Together, these results suggest that CmMYB2 may be a promising gene for the drought and salt tolerance improvement and flowering-time modulation.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 30872064, 31071820, 31071825), and the Program for New Century Excellent Talents in University of Chinese Ministry of Education (Grant No. NCET-10-0492), and the Fundamental Research Funds for the Central Universities (KYJ 200907, KYZ201112), the Program for Hi-Tech Research, Jiangsu, China, Grant (No. BE2010303), 948 Project of Ministry of Agriculture (Grant No. 2008-G3), by Qing Lan Project of Jiangsu Province (Grant No. 2008 [30]), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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  1. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Hong Shan, Sumei Chen, Jiafu Jiang, Fadi Chen, Yu Chen, Chunsun Gu, Peiling Li, Aiping Song, Xirong Zhu & Haishun Gao

  2. Institute of Fishery Science of Nanjing, 183 HanZhongmen Street, Nanjing, 210036, China

    Hong Shan & Guoqin Zhou

  3. College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China

    Ting Li & Xue Yang

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  1. Hong Shan
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Shan, H., Chen, S., Jiang, J. et al. Heterologous Expression of the Chrysanthemum R2R3-MYB Transcription Factor CmMYB2 Enhances Drought and Salinity Tolerance, Increases Hypersensitivity to ABA and Delays Flowering in Arabidopsis thaliana . Mol Biotechnol 51, 160–173 (2012). https://doi.org/10.1007/s12033-011-9451-1

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