Russian Journal of Plant Physiology

, Volume 60, Issue 2, pp 193–199 | Cite as

Dependence of cytokinin distribution in plants on their physical and chemical properties and transpiration rate

  • A. V. Korobova
  • A. N. Vasinskaya
  • G. R. Akhiyarova
  • S. Yu. Veselov
  • G. R. Kudoyarova
  • W. Hartung
Research Papers


The effect of transpiration on cytokinin accumulation and distribution in 7-day-old wheat (Triticum durum Desf.) seedlings grown on nutrient medium supplemented with zeatin or its riboside was studied. The content of cytokinins in plants and nutrient medium was measured by the immunoenzyme analysis; cytokinin distribution between root cells was assessed immunohistochemically using antibodies against zeatin derivatives. The rate of transpiration was reduced 20-fold by plant placing in humid chamber. At normal transpiration, after 6 h of plant incubation on the solution of zeatin, the level of cytokinins in plant tissues increased stronger than after incubation on the solution of zeatin riboside (by 7.3 and 3.5 times, respectively, as compared with control), although the rates of both cytokinin uptake were equal. Most portions of cytokinins were retained in the roots, which was stronger expressed in the case of free zeatin uptake. A decrease in the rate of transpiration did not affect substantially the zeatin absorption from nutrient medium and the total level of cytokinin accumulation in plants, but these indices were sharply decreased in the case of zeatin riboside. In the zone of absorption of both control roots and roots treated with cytokinins, more intense cytokinin immunostaining was observed in the cells of the central cylinder. The interrelation between cytokinin distribution between the cells and apoplast, their inactivation, and transport over the plant and their form (zeatin or zeatin riboside) used for treatment is discussed.


Triticum durum zeatin zeatin riboside transport solid-phase immunoassay immunolocalization transpiration 





relative water content


phosphate-buffered saline


PBS with gelatin and Tween


PBS with Tween


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  1. 1.
    Kulaeva, O.N. and Kusnetsov, V.V., Recent Advances and Horizons of the Cytokinin Studying, Russ. J. Plant Physiol., 2002, vol. 49, pp. 561–574.CrossRefGoogle Scholar
  2. 2.
    Romanov, G.A., How Do Cytokinins Affect the Cell? Russ. J. Plant Physiol., 2009, vol. 56, pp. 268–290.CrossRefGoogle Scholar
  3. 3.
    Kusnetsov, V.V., Oelmöller, R., Sarwat, M.I., Porfirova, S.A., Cherepneva, G.N., and Herrmann, R.G., Cytokinins, Abscisic Acid and Light Affect Accumulation of Chloroplast Proteins in Lupinus luteus Cotyledons without Notable Effect on Steady-State mRNA Levels, Planta, 1994, vol. 194, pp. 318–327.CrossRefGoogle Scholar
  4. 4.
    Mok, M.C., Cytokinins and Plant Development — an Overview, Cytokinins. Chemistry, Activity, and Function, Mok, D.W.S., Mok, M.C, Eds., Boca Raton: CRC, 1994, pp. 155–166.Google Scholar
  5. 5.
    Mok, D.W. and Mok, M.C., Cytokinin Metabolism and Action, Annu. Rev. Plant Physiol. Plant Mol. Biol., 2001, vol. 52, pp. 89–118.PubMedCrossRefGoogle Scholar
  6. 6.
    Korszun, Z.R., Knight, C., and Chen, C.-M., A Stereochemical Model for Cytokinin Activity, FEBS Lett., 1989, vol. 243, pp. 53–56.CrossRefGoogle Scholar
  7. 7.
    Takei, K., Ueda, N., Aoki, K., Kuromori, T., Hirayama, T., Shinozaki, K., Yamaya, T., and Sakakibara, H., AtIPT3 Is a Key Determinant of Nitrate-Dependent Cytokinin Biosynthesis in Arabidopsis, Plant Cell Physiol., 2004, vol. 45, pp. 1053–1062.PubMedCrossRefGoogle Scholar
  8. 8.
    Mok, M.C., Martin, R.C., and Mok, D.W.S., Cytokinins: Biosynthesis, Metabolism and Perception, In Vitro Cell Dev. Biol. — Plant, 2000, vol. 36, pp. 102–107.CrossRefGoogle Scholar
  9. 9.
    Haberer, G. and Kieber, J.J., Cytokinins. New Insights into a Classic Phytohormone, Plant Physiol., 2002, vol. 128, pp. 354–362.PubMedCrossRefGoogle Scholar
  10. 10.
    Sakakibara, H., Cytokinins: Activity, Biosynthesis, and Translocation, Annu. Rev. Plant Biol., 2006, vol. 57, pp. 431–449.PubMedCrossRefGoogle Scholar
  11. 11.
    Lee, D.J., Kim, S., Ha, Y.-M., and Kim, J., Phosphorelay of Arabidopsis Response Regulator 7 (ARR7) at the Putative Phospho-Accepting Site Is Required for ARR7 to Act as a Negative Regulator of Cytokinin Signaling, Planta, 2008, vol. 227, pp. 577–587.PubMedCrossRefGoogle Scholar
  12. 12.
    Faiss, M.L., Zalubilova, J.L., Strnad, M.L., and Schmülling, T., Conditional Transgenic Expression of the IPT Gene Indicates a Function for Cytokinins in Paracrine Signalling in Whole Tobacco Plants, Plant J., 1997, vol. 12, pp. 401–415.PubMedCrossRefGoogle Scholar
  13. 13.
    Sakakibara, H., Nitrate-Specific and Cytokinin-Mediated Nitrogen Signaling Pathways in Plants, J. Plant Res., 2003, vol. 116, pp. 253–257.PubMedCrossRefGoogle Scholar
  14. 14.
    Shtratnikova, V.Yu. and Kulaeva, O.N., Cytokinin-Dependent Expression of the ARR5::GUS Construct during Transgenic Arabidopsis Growth, Russ. J. Plant Physiol., 2008, vol. 55, pp. 756–764.CrossRefGoogle Scholar
  15. 15.
    Vysotskaya, L.B., Veselov, S.Yu., and Kudoyarova, G.R., Effect on Shoot Water Relations, and Cytokinin and Abscisic Acid Levels of Inducing Expression of a Gene Coding for Isopentenyltransferase in Roots of Transgenic Tobacco Plants, J. Exp. Bot., 2010, vol. 61, pp. 3709–3717.PubMedCrossRefGoogle Scholar
  16. 16.
    Hirose, N.L., Makita, N.L., Yamaya, T.L., and Sakakibara, H., Functional Characterization and Expression Analysis of a Gene, OsENT2, Encoding an Equilibrative Nucleoside Transporter in Rice Suggest a Function in Cytokinin Transport, Plant Physiol., 2005, vol. 138, pp. 196–206.PubMedCrossRefGoogle Scholar
  17. 17.
    Traub, M., Flörchinger, M., Piecuch, J., Kunz, H., Weise-Steinmetz, A., Deitmer, J.W., Ekkehard Neuhaus, H., and Möhlmann T., The Fluorouridine Insensitive 1 (FUR1) Mutant Is Defective in Equilibrative Nucleoside Transporter 3 (ENT3), and Thus Represents An Important Pyrimidine Nucleoside Uptake System in Arabidopsis thaliana, Plant J., 2007, vol. 49, pp. 855–864.PubMedCrossRefGoogle Scholar
  18. 18.
    Hirose, N., Takei, K., Kuroha, T., Kamada-Nobusada, T., Hayashi, H., and Sakakibara, H., Regulation of Cytokinin Biosynthesis, Compartmentalization and Translocation, J. Exp. Bot., 2008, vol. 59, pp. 75–83.PubMedCrossRefGoogle Scholar
  19. 19.
    Miyawaki, K., Matsumoto-Kitano, M., and Kakimoto, T., Expression of Cytokinin Biosynthetic Isopentenyltransferase Genes in Arabidopsis: Tissue Specificity and Regulation by Auxin, Cytokinin, and Nitrate, Plant J., 2004, vol. 37, pp. 128–138.PubMedCrossRefGoogle Scholar
  20. 20.
    Sauter, A. and Hartung, W., The Contribution of Internode and Mesocotyl Tissues to Root-to-Shoot Signalling of Abscisic Acid, J. Exp. Bot., 2002, vol. 53, pp. 297–302.PubMedCrossRefGoogle Scholar
  21. 21.
    Akhiyarova, G.R. and Arkhipova, T.N., Exogenous Zeatin Accumulation in Wheat Root Cells Shows Its Role in the Regulation of Cytokinin Transport, Tsitologya, 2010, vol. 52, pp. 1024–1030.Google Scholar
  22. 22.
    Kudoyarova, G.R., Vysotskaya, L.B., Cherkozyanova, A., and Dodd, I.C., Effect of Partial Rootzone Drying on the Concentration of Zeatin-Type Cytokinins in Tomato (Solanum lycopersicum L.) Xylem Sap and Leaves, J. Exp. Bot., 2007, vol. 58, pp. 161–168.PubMedCrossRefGoogle Scholar
  23. 23.
    Veselov, S.Yu., Valcke, R., van Onkelen, H., and Kudoyarova, G.R., Cytokinin Content and Location in the Leaves of the Wild-Type and Transgenic Tobacco Plants, Russ. J. Plant Physiol., 1999, vol. 46, pp. 26–31.Google Scholar
  24. 24.
    Kudoyarova, G.R., Veselov, S.Yu., Karavaiko, N.N., Guli-zade, V.Z., Cheredova, E.P., Mustafina, A.R., Moshkov, I.E., and Kulaeva, O.N., Immune-Enzyme System for Cytokinin Determination, Sov. Plant Physiol., 1990, vol. 37, pp. 193–199.Google Scholar
  25. 25.
    Glover, B.J., Torney, K., Wilkins, C.G., and Hanke, D.E., Cytokinin Independent-1 Regulates Levels of Different Forms of Cytokinin in Arabidopsis and Mediates Response to Nutrient Stress, J. Plant Physiol., 2008, vol. 165, pp. 251–261.PubMedCrossRefGoogle Scholar
  26. 26.
    Steudle, E., Water Uptake by Roots: An Integration of Views, Plant Soil, 2000, vol. 226, pp. 15–56.CrossRefGoogle Scholar
  27. 27.
    Brugiere, N., Jiao, S.P., Hantke, S., Zinselmeier, C., Roessler, J.A., Niu, X.M., Jones, R.J., and Habben, J.E., Cytokinin Oxidase Gene Expression in Maize Is Localized to the Vasculature, and Is Induced by Cytokinins, Abscisic Acid, and Abiotic Stress, Plant Physiol., 2003, vol. 132, pp. 1228–1240.PubMedCrossRefGoogle Scholar
  28. 28.
    Schraut, D., Ullrich, C.I., and Hartung, W., Lateral ABA Transport in Maize Roots (Zea mays): Visualization by Immunolocalization, J. Exp. Bot., 2004, vol. 55, pp. 1635–1641.PubMedCrossRefGoogle Scholar
  29. 29.
    Sabatini, S., Beis, D., Wolkenfelt, H., Murfett, J., Guilfoyle, T., Malamy, J., Benfey, P., Leyser, O., Bechtold, N., Weisbeek, P., and Scheres, B., An Auxin-Dependent Distal Organizer of Pattern and Polarity in the Arabidopsis Root, Cell, 1999, vol. 99, pp. 463–472.PubMedCrossRefGoogle Scholar
  30. 30.
    Burkle, L., Cedzich, A., Dopke, C., Stransky, H., Okumoto, S., Gillissen, B., Kuhn, C., and Frommer, W.B., Transport of Cytokinins Mediated by Purine Transporters of the PUP Family Expressed in Phloem, Hydathodes, and Pollen of Arabidopsis, Plant J., 2003, vol. 34, pp. 13–26.PubMedCrossRefGoogle Scholar
  31. 31.
    Cedzich, A., Stransky, H., Schulz, B., and Frommer, W.B., Characterization of Cytokinin and Adenine Transport in Arabidopsis Cell Cultures, Plant Physiol., 2008, vol. 148, pp. 1857–1867.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • A. V. Korobova
    • 1
  • A. N. Vasinskaya
    • 1
  • G. R. Akhiyarova
    • 1
  • S. Yu. Veselov
    • 2
  • G. R. Kudoyarova
    • 1
  • W. Hartung
    • 3
  1. 1.Institute of Biology, Ufa Research CenterRussian Academy of SciencesUfaRussia
  2. 2.Bashkir State UniversityUfaRussia
  3. 3.Julius-von-Sachs-Institut für Biowissenschaften der Universität WurzburgWurzburgGermany

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