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Peripheral light-harvesting LH2 complex can be assembled in cells of nonsulfur purple bacterium Rhodoblastus acidophilus without carotenoids

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Abstract

The effect of carotenoids on the assembly of LH2 complex in cells of the purple nonsulfur bacterium Rhodoblastus acidophilus was investigated. For this purpose, the bacterial culture was cultivated with an inhibitor of carotenoid biosynthesis — 71 µM diphenylamine (DPA). The inhibitor decreased the level of biosynthesis of the colored carotenoids in membranes by ~58%. It was found that a large amount of phytoene was accumulated in them. This carotenoid precursor was bound nonspecifically to LH2 complex and did not stabilize its structure. Thermostability testing of the isolated LH2 complex together with analysis of carotenoid composition revealed that the population of this complex was heterogeneous with respect to carotenoid composition. One fraction of the LH2 complex with carotenoid content around 90% remains stable and was not destroyed under heating for 15 min at 50°C. The other fraction of LH2 complex containing on average less than one molecule of carotenoid per complex was destroyed under heating, forming a zone of free pigments (and polypeptides). The data suggest that a certain part of the LH2 complexes is assembled without carotenoids in cells of the nonsulfur bacterium Rbl. acidophilus grown with DPA. These data contradict the fact that the LH2 complex from nonsulfur bacteria cannot be assembled without carotenoids, but on the other hand, they are in good agreement with the results demonstrated in our earlier studies of the sulfur bacteria Allochromatium minutissimum and Ectothiorhodospira haloalkaliphila. Carotenoidless LH2 complex was obtained from these bacteria with the use of DPA (Moskalenko, A. A., and Makhneva, Z. K. (2012) J. Photochem. Photobiol., 108, 1–7; Ashikhmin, A., et al. (2014) Photosynth. Res., 119, 291–303).

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Abbreviations

BChl:

bacteriochlorophyll

DM:

dodecyl-ß-D-maltopyranoside

DPA:

diphenylamine

DPA sample (membrane, complex):

sample obtained from cells grown with 71 µM DPA

LH:

light harvesting

RC:

reaction center

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    M. A. Bol’shakov, A. A. Ashikhmin, Z. K. Makhneva & A. A. Moskalenko

Authors
  1. M. A. Bol’shakov
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  2. A. A. Ashikhmin
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  3. Z. K. Makhneva
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  4. A. A. Moskalenko
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Correspondence to A. A. Moskalenko.

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Original Russian Text © M. A. Bol’shakov, A. A. Ashikhmin, Z. K. Makhneva, A. A. Moskalenko, 2015, published in Biokhimiya, 2015, Vol. 80, No. 9, pp. 1420–1430.

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Bol’shakov, M.A., Ashikhmin, A.A., Makhneva, Z.K. et al. Peripheral light-harvesting LH2 complex can be assembled in cells of nonsulfur purple bacterium Rhodoblastus acidophilus without carotenoids. Biochemistry Moscow 80, 1169–1177 (2015). https://doi.org/10.1134/S0006297915090072

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  • DOI: https://doi.org/10.1134/S0006297915090072

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