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Enzyme-linked lectinosorbent assay of Escherichia coli and Staphylococcus aureus

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Abstract

In this study, we developed a microplate sandwich analysis of Escherichia coli and Staphylococcus aureus bacterial pathogens based on the interaction of their cell wall carbohydrates with natural receptors called lectins. An immobilized lectin-cell-biotinylated lectin complex was formed in this assay. Here, we studied the binding specificity of several plant lectins to E. coli and S. aureus cells, and pairs characterized by high-affinity interactions were selected for the assay. Wheat germ agglutinin and Ricinus communis agglutinin were used to develop enzyme-linked lectinosorbent assays for E. coli and S. aureus cells with the detection limits of 4 × 106 and 5 × 105 cells/mL, respectively. Comparison of the enzyme-linked immonosorbent assay and the enzyme-linked lectinosorbent assay demonstrated no significant differences in detection limit values for E. coli. Due to the accessibility and universality of lectin reagents, the proposed approach is a promising tool for the control of a wide range of bacterial pathogens.

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Authors and Affiliations

  1. A.N. Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences, Moscow, 119071, Russia

    O. D. Hendrickson, N. I. Smirnova, A. V. Zherdev & B. B. Dzantiev

  2. G.Kh. Bunatyan Institute of Biochemistry, National Academy of Sciences of Armenia, Erevan, 0014, Armenia

    V. K. Gasparyan

Authors
  1. O. D. Hendrickson
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  2. N. I. Smirnova
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  3. A. V. Zherdev
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  4. V. K. Gasparyan
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  5. B. B. Dzantiev
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Correspondence to B. B. Dzantiev.

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Original Russian Text © O.D. Hendrickson, N.I. Smirnova, A.V. Zherdev, V.K. Gasparyan, B.B. Dzantiev, 2017, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2017, Vol. 53, No. 1, pp. 96–103.

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Hendrickson, O.D., Smirnova, N.I., Zherdev, A.V. et al. Enzyme-linked lectinosorbent assay of Escherichia coli and Staphylococcus aureus . Appl Biochem Microbiol 53, 107–113 (2017). https://doi.org/10.1134/S0003683817010082

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  • DOI: https://doi.org/10.1134/S0003683817010082

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