Skip to main content
SpringerLink
Log in

Enzyme-linked lectinosorbent assay (ELLA) for detectingBacillus anthracis

  • Short Reports
  • Published:
European Journal of Clinical Microbiology Aims and scope Submit manuscript

Abstract

An enzyme-linked lectinosorbent assay (ELLA) has been developed for the rapid identification ofBacillus anthracis. Using two different lectin-conjugates, the ELLA test can differentiateBacillus anthracis from closely related species, such asBacillus cereus andBacillus cereus var.mycoides, in approximately two hours. In addition to having high specificity, the test can also detect small numbers of the bacterium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Doyle, R., Keller, K.: Lectins in diagnostic microbiology. European Journal of Clinical Microbiology 1984, 3: 4–9.

    PubMed  Google Scholar 

  2. Pistole, T. G.: Interactions of bacteria and fungi with lectins and lectin-like substances. Annual Review of Microbiology 1981, 35: 85–112.

    PubMed  Google Scholar 

  3. Schaefer, R., Kellet, K. F., Doyle, R. J.: Lectins in diagnostic microbiology: use of wheat germ agglutinin for the laboratory identification ofNeisseriagonorrhoeae. Journal of Clinical Microbiology 1979, 10: 669–672.

    PubMed  Google Scholar 

  4. Cole, H. B., Ezzell, J. W., Keller, K. F., Doyle, R. J.: Differentiation ofBacillus anthracis and otherBacillus species by use of lectins. Journal of Clinical Microbiology 1984, 19: 48–53.

    PubMed  Google Scholar 

  5. Engvall, E., Perlmann, P.: Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971, 8: 871–874.

    Google Scholar 

  6. Hilderbrand, R. L.: Elisa (enzyme-linked immunosorbent assay). In: Rytel, M. W. (ed.): Rapid diagnosis in infectious disease. Chemical Rubber Company Press, Boca Raton, FL, 1979, p. 71–88.

    Google Scholar 

  7. Feeley, J. C., Patton, C. M.:Bacillus. In: Lennette, E. H., Balows, A., Hausler, W. J., Truant, J. P. (ed.): Manual of clinical microbiology. American Society for Microbiology, Washington, D.C., 1980, p. 145–149.

    Google Scholar 

  8. Goldstein, I. J., Hayes, C. E.: The lectins: carbohydrate-binding proteins of plants and animals. Advances in Carbohydrate Chemistry and Biochemistry 1978, 35: 127–340.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Louisville, Health Sciences Center, 40292, Louisville, Kentucky, USA

    K. Graham, K. Keller & R. Doyle

  2. Division of Bacteriology, US Army Medical Institute of Infectious Diseases, 21701, Fort Detrick, Maryland, USA

    J. Ezzell

Authors
  1. K. Graham
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Keller
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Ezzell
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Doyle
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Graham, K., Keller, K., Ezzell, J. et al. Enzyme-linked lectinosorbent assay (ELLA) for detectingBacillus anthracis . Eur. J, Clin. Microbiol. 3, 210–212 (1984). https://doi.org/10.1007/BF02014881

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02014881

Keywords

Search

Navigation