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An increase of curdlan productivity by integration of carbon/nitrogen sources control and sequencing dual fed-batch fermentors operation

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Abstract

Curdlan is produced by Agrobacterium sp. ATCC 31749 under nitrogen-limited conditions not associated with cell growth. A novel curdlan production process was developed based on the different nutrient requirements for microbial cell growth and its efficiency was increased by integrating carbon/nitrogen sources control and sequencing dual fed-batch fermentors operation. By feeding ammonium solution to supply abundant nitrogen source and controlling pH in Fermentor I, cell growth was accelerated. High cell density of 29 g/L was attained. The culture broth in Fermentor I was then inoculated into sequencing Fermentor II which alleviated the high requirement for dissolved oxygen and accumulation of inhibitory metabolic by-products during curdlan production. Fermentor I promoted cell growth. Curdlan production started instantaneously in Fermentor II. By feeding nutrient solution with high carbon/nitrogen ratio and NaOH solution for pH adjustment, a feasible and optimal curdlan production process was formulated. The productivity, conversion efficiency and curdlan yield were achieved of 0.98 g/(L h), 57% (w) and 67 g/L, respectively. Such novel process can be scaled up for significant cost reduction at the industrial level.

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Authors and Affiliations

  1. Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, 214122, China

    Z. Y. Zheng, Y. Jiang, X. B. Zhan, L. W. Ma, J. R. Wu, L. M. Zhang & C. C. Lin

Authors
  1. Z. Y. Zheng
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  2. Y. Jiang
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  3. X. B. Zhan
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  4. L. W. Ma
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  5. J. R. Wu
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  6. L. M. Zhang
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  7. C. C. Lin
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Corresponding author

Correspondence to X. B. Zhan.

Additional information

Published in Russian in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 1, pp. 44–51.

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Zheng, Z.Y., Jiang, Y., Zhan, X.B. et al. An increase of curdlan productivity by integration of carbon/nitrogen sources control and sequencing dual fed-batch fermentors operation. Appl Biochem Microbiol 50, 35–42 (2014). https://doi.org/10.1134/S000368381401013X

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  • DOI: https://doi.org/10.1134/S000368381401013X

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