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Regulatory Sequences for Constitutive, Tissue-Specific, and Induced Expression of Transgenes in Ornamental Plants

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Abstract

Genetic engineering allows for an extension beyond the limits of a species' natural variability. This enables the production of ornamental plants with novel colors and shapes of flowers, enhanced resistance, and a complex of characteristics gaining aesthetic and economic advantages. As a rule, success in achievement of desirable effects requires changes in the expression patterns of particular genes. A key regulatory element determining the level and tissue and temporal specificity in gene expression is a promoter. Therefore, an appropriate promoter must be chosen in the first place to build the planned structures of genetic constructs for transgene expression in plants. In recent years, many novel constitutive, tissue-specific, and induced promoters of plant origin have been explored. They may broaden advancements for derivation of novel forms and cultivars by means of a strictly directed expression of the transferred genes. The review discusses literary data on promoter application to biotechnology for the cases of ornamental plants.

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ACKNOWLEDGMENTS

We thank the reviewers for the attentive consideration of the manuscript and critical comments made.

Funding

The work was supported by the budget project no. 0324-2019-0039.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    O. G. Smirnova, V. K. Shumny & A. V. Kochetov

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  1. O. G. Smirnova
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  2. V. K. Shumny
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  3. A. V. Kochetov
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Correspondence to O. G. Smirnova.

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Translated by A. Aver’yanov

Abbreviations: CaMV35S—promoter of 35S cauliflower mosaic RNA virus; CHS—chalcone synthase; CMV—cucumber mosaic virus; DFR—dihydroflavonol 4-reductase; DREB—dehydration responsive element binding protein; F3H—flavanone 3-hydroxylase; F3'5'H—flavonoid 3',5'-hydroxylase; GUS—β-glucuronidase; SAG12—senescence-associated gene; SHI—short internodes gene; uidA—β-glucuronidase gene; 5'-UTR—5'-untranslated region.

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Smirnova, O.G., Shumny, V.K. & Kochetov, A.V. Regulatory Sequences for Constitutive, Tissue-Specific, and Induced Expression of Transgenes in Ornamental Plants. Russ J Plant Physiol 66, 679–693 (2019). https://doi.org/10.1134/S1021443719050182

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