Advertisement

Applied Biochemistry and Microbiology

, Volume 54, Issue 6, pp 639–648 | Cite as

Identification and Morphological-Physiological Characterization of Astaxanthin Producer Strains of Haematococcus pluvialis from the Black Sea Region

  • E. S. Chelebieva
  • N. V. Dantsyuk
  • K. A. Chekanov
  • I. N. Chubchikova
  • I. V. Drobetskaya
  • G. S. Minyuk
  • E. S. Lobakova
  • A. E. SolovchenkoEmail author
Article
  • 67 Downloads

Abstract

The molecular genetic, morphometric, and physiological-biochemical characteristics of three isolates of the carotenogenic microalga Haematococcus sp. obtained in different regions of the Caucasus and Crimea were studied. According to the results of light microscopy and PCR analysis of 18s rDNA gene fragments, all isolates were identified as Haematococcus pluvialis (clade Chlorogonia, order Volvocales, class Chlorophyceae). It is shown that the strains isolated in the mountainous and valley areas of the Black Sea region differ in important characteristics relevant for mass cultivation: cell size, growth rate, stress tolerance, mass fraction of total carotenoids and astaxanthin in dry matter, and their yield per liter of the start culture. The H. pluvialis isolates most promising for further research and the development of a cultivation technology for the Black Sea regions of the Caucasus and Crimea were strains IMBR-3 (Sevastopol) and IMBR-1 (Adler), which were characterized by the highest astaxanthin production (16.77 ± 1.32 and 17.12 ± 0.72 mg/(L day), respectively).

Keywords:

Haematococcus pluvialis carotenoids astaxanthin 

Notes

REFERENCES

  1. 1.
    Han, D., Li, Y., and Hu, Q., Handbook of Microalgal Culture: Applied Phycology and Biotechnology, Richmond, A. and Hu, Q., Eds., New York: :John Wiley and Sons, 2013, pp. 388–405.Google Scholar
  2. 2.
    Shah, M.M.R., Liang, Y., Cheng, J.J., and Daroch, M., Front. Plant Sci., 2016, vol. 7, art. 531, pp. 1–28. doi 10.3389/fpls.2016.00531Google Scholar
  3. 3.
    Moretti, V.M., Mentasti, T., Bellagamba, F., Luzzana, U., Caprino, F., Turchini, G.M., Giani, I., and Valfre, F., Food. Add. Contam., 2006, vol. 23, pp. 1056–1063.CrossRefGoogle Scholar
  4. 4.
    Noroozi, M., Omar, H., Napis, S., Hejazi, M.A., and Tan, S.G., African J. Biotechnol., 2012, vol. 11, no. 84, pp. 15049–15059.Google Scholar
  5. 5.
    Gonzalez, M.A., Cifuentes, A.S., and Gomez, P.I., Gayana. Botanica, 2009, vol. 66, no. 1, pp. 58–70.CrossRefGoogle Scholar
  6. 6.
    Gao, Z., Meng, C., Chen, Y.C., Ahmed, F., Mangott, A., Schenk, P.M., and Li, Y., J. Appl. Phycol., 2015, vol. 27, no. 5, pp. 1853–1860.CrossRefGoogle Scholar
  7. 7.
    Minyuk, G.S., Terent’eva, N.V., and Drobetskaya, I.V., Algologiya, 2007, vol. 17, no. 2, pp. 148–159.Google Scholar
  8. 8.
    Fabregas, J., Otero, A., Maseda, A., and Dominguez, A., J. Biotechnol., 2001, vol. 89, pp. 65–71.CrossRefGoogle Scholar
  9. 9.
    Andersen, R.A., Algal Culturing Techniques, San Diego: Academic Press, 2005.Google Scholar
  10. 10.
    Buchheim, M.A., Sutherland, D.M., Buchheim, J.A., and Wolf, M., Eur. J. Phycol., 2013, vol. 48, no. 3, pp. 318–329.CrossRefGoogle Scholar
  11. 11.
    Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S., Mol. Biol. EV, 2013, vol. 30, pp. 2725–2729.CrossRefGoogle Scholar
  12. 12.
    Edgar, RC., Nucleic Acids Res., 2004, vol. 32, no. 5, pp. 1792–1797.CrossRefGoogle Scholar
  13. 13.
    Aldrich, J.R.A., Stat. Sci., 1997, vol. 12, no. 3, pp. 162–176.CrossRefGoogle Scholar
  14. 14.
    Kimura, M., J. Mol. Evol., 1980, vol. 16, pp. 111–120.CrossRefGoogle Scholar
  15. 15.
    Felsenstein, J., Evolution, 1985, vol. 39, pp. 783–791.CrossRefGoogle Scholar
  16. 16.
    Britton, G., in Eds.: Britton, G., Liaasen-Jensen, S., and Pfander, H., Carotenoids, vol. 1 A: Isolation and Analysis, Basel: Birkauser Verlag, 1995, pp. 199–225.Google Scholar
  17. 17.
    Lichtenthaler, H.K., Meth. Enzymol., 1987, vol. 148, pp. 350–382.CrossRefGoogle Scholar
  18. 18.
    Drobetskaya, I.V., Chubchikova, I.N., Borovkov, A.B., and Minyuk, G.S., Ekol. Morya, 2010, vol. 79, pp. 50–56.Google Scholar
  19. 19.
    Pocock, M.A., Trans. R. Sos. S. Afr., 1960, vol. 36, no. 1, pp. 5–55.CrossRefGoogle Scholar
  20. 20.
    Gu, W., Xie, X., Gao, S., Zhou, W., and Pan, G., PLoS One, 2013, vol. 8, no. 7.Google Scholar
  21. 21.
    Pentecost, A., in The Freshwater Algal Flora of the British Isles: An Identification Guide to Freshwater and Terrestrial Algae, John, D.M., Whitton, B.A., and Brook, A.J., Eds., Cambridge: CUP, 2002, vol. 1, pp. 381–409.Google Scholar
  22. 22.
    Klochkova, T.A., Kwak, M.S., Han, J.W., Motomura, T., Nagasato, C., and Kim, G.H., Algae, 2013, vol. 28, no. 2, pp. 185–192.CrossRefGoogle Scholar
  23. 23.
    Chekanov, K., Lobakova, E., Selyakh, I., Semenova, L., Sidorov, R., and Solovchenko, A., Mar. Drugs, 2014, vol. 12, no. 8, pp. 4504–4520.CrossRefGoogle Scholar
  24. 24.
    Elliot, F.M., Arch. Protistenk., 1934, vol. 82, pp. 250–273.Google Scholar
  25. 25.
    Zhang, C., Liu, J., and Zhang, L., Chinese J. Ocean. Limnol., 2017, vol. 35, no. 5, pp. 1205–1211.CrossRefGoogle Scholar
  26. 26.
    Proschold, T. and Leliaert, F., in Systematics Association Special Volume Series, Brodie, J. and Lewis, J.M., Eds., Boca Raton, FL: CRC Press, 2007, pp. 123–153.Google Scholar
  27. 27.
    Triki, A., Maillard, P., and Gudin, C., Phycologia, 1997, vol. 36, no. 3, pp. 190–194.CrossRefGoogle Scholar
  28. 28.
    Liu, J.G., Yin, M.Y., Zhang, J.P., Meng, Z.C., and Bourne, W.F., Oceanol. Limnol. Sinica, 2000, vol. 31, no. 2, pp. 145–150.Google Scholar
  29. 29.
    Masyuk, N.P. and Demchenko, E.N., Algologiya, 2001, vol. 11, no. 3, pp. 298–310.Google Scholar
  30. 30.
    Minyuk, G.S., Erokhin, V.E., Gordienko, A.P., Terent’eva, N.V., Chubchikova, I.N., and Drobetskaya, I.V., in Mikrovodorosli Chernogo morya: problemy sokhraneniya bioraznoobraziya i biotekhnologicheskogo ispol’zovaniya (Microalgae of the Black Sea: Problems of Biodiversity Conservation and Biotechnological Use), Sevastopol: EKOSI-Gidrofizika, 2008, pp. 353–392.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • E. S. Chelebieva
    • 1
  • N. V. Dantsyuk
    • 1
  • K. A. Chekanov
    • 2
  • I. N. Chubchikova
    • 1
  • I. V. Drobetskaya
    • 1
  • G. S. Minyuk
    • 1
  • E. S. Lobakova
    • 2
  • A. E. Solovchenko
    • 2
    Email author
  1. 1.Kovalevsky Institute of Marine Biological Research, Russian Academy of SciencesSevastopolRussia
  2. 2.Lomonosov Moscow State UniversityMoscowRussia

Personalised recommendations