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Morphological and Molecular Genetic Analysis of Green Microalgal Strains Isolated from Commercial Biological Products Based on “Living Сhlorella”

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Abstract

In the present study, Chlorella-like strains of green microalgae from various commercial biological products were studied for the first time using morphological and molecular genetic analyses. It was found that representatives of the Chlorella clade or sister Parachlorella clade were present in all analyzed samples. In addition, microalgae from the genera Edaphochlorella, Chloroidium, Edaphochloris, and Muriella belonging to the Trebouxiophyceae class and Coelastrella and Chromochloris belonging to the Chlorophyceae class were found. According to direct light microscopy data, filamentous cyanobacteria were observed in all preparations, and the presence of diatoms was also noted in the Gera biopreparation. Since the correct identification of green microalgae based on phenotypic traits is very difficult due to their scarcity, lack of information, and variability, the control of the composition of microalgae in biological products should be carried out using modern molecular genetic methods.

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Funding

The study was funded by the Russian Foundation for Basic Research, project no. 19-34-60002.

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Authors and Affiliations

  1. Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290, Pushchino, Russia

    E. S. Krivina & A. D. Temraleeva

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  1. E. S. Krivina
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  2. A. D. Temraleeva
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Correspondence to E. S. Krivina.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by P. Kuchina

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Krivina, E.S., Temraleeva, A.D. Morphological and Molecular Genetic Analysis of Green Microalgal Strains Isolated from Commercial Biological Products Based on “Living Сhlorella”. Moscow Univ. Biol.Sci. Bull. 77, 147–151 (2022). https://doi.org/10.3103/S0096392522030063

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