Abstract
Microbial surfactants are a structurally diverse group of compounds consisting of hydrophilic and hydrophobic domains and which partition preferentially at interfaces. Biosurfactants are of increasing interest commercially as substitutes for synthetic surfactants particularly for environmental applications. This article discusses recent progress in the genetic and biochemical analysis of biosurfactant synthesis as well as the current status of fermentation technologies.
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References
Israelachvili, J.N. 1985. Intermolecular and Surface Forces. Academic Press, NY.
Scriven, L.E. 1976. Equilibrium bi-continuous structures. In: Micellization, Solubilization, and Microemulsions. Mittal, K.L. (Ed.). Plenum Press, NY .
Cooper, D.G.,1986. Biosurfactants Microbiol. Sci. 3: 145–149.
Haferburg, D., Hommel, R., Claus, R. and Kleber, H.-P. 1986. Extracellular microbial lipids as biosurfactants. Advanc. Biochem. Eng. Biotechnol. 33: 54–93.
Cooper, D.G. and Zajic, J.E. 1980. Surface-active compounds from microorganisms. Adv. Appl. Microbiol. 26: 229–253.
Horbett, T.A. 1988. Molecular origins of the surface activity of proteins. Protein Eng. 2: 172–174.
Ramsay, B., McCarthy, J., Guerra-Santos, L., Käppeli, O., Feichter, A. and Margaritis, A. 1988. Biosurfactant production and diauxic growth of Rhodococcus aurantiacus when using n-alkanes as the carbon cource. Can. J. Microbiol. 34: 1209–1212.
Finnerty, W.R. and Singer, M.E. 1984. A microbial biosurfactant— physiology, biochemistry, and applications. Dev. Ind. Microbiol. 25: 31–46.
Hommel, R., Stüwer, O., Stuber, W., Haferburg, D. and Kleber, H.-P. 1987. Production of water-soluble surface-active exolipids by Torulopsis apicola . Appl. Microbiol. Biotechnol. 26: 199–205.
Kim, J.-S., Powalla, M., Lang, S., Wagner, F., Lunsdorf, H. and Wray, V. 1990. Microbial glycolipid production under nitrogen limitation and resting cell conditions. J. Biotechnol. 13: 257–266.
Guerra-Santos, L.H., Kappeli, O. and Fiechter, A. 1986. Dependence of Pseudomonas aeruginosa continuous culture biosurfactant production on nutritional and environmental factors. Appl. Microbiol. Biotechnol. 24: 443–448.
Reiling, H.E., Thanei-Wyss, U., Guerra-Santos, L.H., Hirt, R., Kappeli, O. and Fiechter, A. 1986. Pilot plant production of rhamnolipid biosurfactant by Pseudomonas aeruginosa . Appl. Environ. Microbiol. 51: 985–989.
Robert, M., Mercadé, M.E., Bosch, M.P., Parra, J.L., Espuny, M.J., Manresa, M.A. and Guinea, J. 1989. Effect of the carbon source on biosurfactant production by Pseudomonas aeruginosa 44T1. Biotechnol. Lett. 11: 871–874.
Matsuyama, T., Kaneda, K., Ishizuka, I., Toida, T. and Yano, I. 1990. Surface-active novel glycolipid and linked 3-hydroxy fatty acids produced by Serratia rubidaea . J. Bacteriol. 172: 3015–3022.
Cooper, D.G. and Paddock, D.A. 1984. Production of a biosurfactant from Torulopsis bombicola . Appl. Environ. Microbiol. 47: 173–176.
Göbbert, U., Lang, S. and Wagner, F. 1984. Sophorose lipid formation by resting cells of Torulopsis bombicola . Biotechnol. Lett. 6: 225–230.
Asmer, H.-J., Lang, S., Wagner, F. and Wray, V. 1988. Microbial production, structure elucidation and bioconversion of sophorose lipids. J. Amer. Oil Chem. Soc. 65: 1460–1466.
Rapp, P., Bock, H., Wray, V. and Wagner, F., 1979. rmation, isolation and characterization of trehalose dimycolates from Rhodococcus eryihropotis grown on n-alkane. J. Gen. Microbiol. 115: 491–503.
Powalla, M., Lang, S. and Wray, V. 1989. Penta- and disaccharide lipid formation by Nocardia corynebacteroidesgrown on n-alkanes. Appl. Microbiol. Biotechnol. 31: 473–479.
Jenneman, G.E., McInerney, M.J., Knapp, R.M., Clark, J.B., Ferro, J.M., Revus, D.E. and Menzie, D.E. 1983. A halotolerant, biosurfactant-producing Bacillus species potentially useful for enhanced oil recovery. Dev. Ind. Microbiol. 24: 485–492.
Javaheri, M., Jenneman, G.E., McInerney, M.J. and Knapp, R.M. 1985. Anaerobic production of a biosurfactant by Bacillus licheniformis JF-2 Appl. Environ. Microbiol. 50: 698–700.
McInerney, M.J., Javaheri, M. and Nagle, D.P. 1990. Properties of the biosurfactant produced by Bacillus licheniformis strain JF-2. J. Ind. Microbiol. 5: 95–102.
Lin, S.C., Goursaud, J.-C., Kramer, P.J., Georgiou, G. and Sharma, M.M. 1990. Production of biosurfactant by Bacillus licheniformis strain JF-2. In: Microbial Enhancement of Oil Recovery—Recent Advances. Donaldson, E. C. (Ed.). Elsevier Science Publishers, Amsterdam.
Horowitz, S., Gilbert, J.N. and Griffin, W.M. 1990. Isolation and characterization of a surfactant produced by Bacillus licheniformis 86. J. Ind. Microbiol. 6: 243–248.
Neu, T.R., Hartner, T. and Poralla, K. 1990. Surface active properties of viscosin: a peptidolipid antibiotic. Appl. Microbiol. Biotechnol. 32: 518–520.
Matsuyama, T., Fujita, M. and Yano, I. 1985. Wetting agent produced by Serratia marcescens . FEMS Microbiol. Lett. 28: 125–129.
Matsuyama, T., Murakami, T., Fujita, M., Fujita, S. and Yano, I. 1986. Extracellular vesicle formation and biosurfactant production by Serratia marcescens . J. Gen. Microbiol. 132: 865–875.
Arima, K., Kakinuma, A. and Tamura, G., 1968. rfactin, a crystalline peptidelipid surfactant produced by Bacillus subtilis: isolation, characterization and its inhibition of fibrin clot formation Biochem. Biophys. Res. Com. 31: 488–494.
Cooper, D.G., MacDonald, C.R., Duff, S.J.B. and Kosaric, N. 1981. Enhanced production of surfactin from Bacillus subtilis by continuous product removal and metal cation additions. Appl. Environ. Microbiol. 42: 408–412.
Sheppard, J.D. and Mulligan, C.N. 1987. The production of surfactin by Bacillus subtilis grown on peat hydrolysate. Appl. Microbiol. Biotechnol. 27: 110–116.
Sandrin, C., Peypoux, F. and Michel, G. 1990. Coproduction of surfactin and iturin A, lipopeptides with surfactant and antifungal properties, by Bacillus subtilis . Biotechnol. Appl. Biochem. 12: 370–375.
Persson, A., Österberg, E. and Dostalek, M. 1988. Biosurfactant production by Pseudomonas fluorescens 378: growth and product characteristics. Appl. Microbiol. Biotechnol. 29: 1–4.
Pareilleux, A. 1979. Hydrocarbon assimilation by Candida lipolytica: Formation of a biosurfactant; effects on respiratory activity and growth. Eur. J. Appl. Microbiol. Biotechnol. 8: 91–101.
Zajic, J.E., Guignard, H. and Gerson, D.F. 1977. Properties and bio'degradation of a bioemulsifier from Corynebacterium hydrocarboclastus . Biotechnol. Bioeng. 19: 1303–1320.
Jones, G.E. and Starkey, R.L. 1961. Surface-active substances produced by Thiobacillus thiooxidans J. Bacteriol. 82: 788–789.
Beebe, J.L. and Umbreit, W.W. 1971. Extracellular lipid of Thiobacillus thiooxidans J. Bacteriol. 108: 612–614.
Cooper, D.G., Zajic, J.E. and Gerson, D.F. 1979. Production of surface-active lipids by Corynebacterium lepus . Appl. Environ. Microbiol. 37: 4–10.
Gerson, D.F. and Zajic, J.E. 1978. Surfactant production from hydrocarbons by Corynebacterium lepus, sp. nov. and Pseudomonas asphaltmicus, sp. nov. Dev. Ind. Microbiol. 19: 577–599.
MacDonald, C.R., Cooper, D.G. and Zajic, J.E. 1981. Surface-active lipids from Nocardia erythropolis grown on hydrocarbons. Appl. Environ. Microbiol. 41: 117–123.
Hisatsuka, K., Nakahara, T., Sano, N. and Yamada, K. 1971. Formation of rhamnolipid by Pseudomonas aeruginosa and its function in hydrocarbon fermentation Agr. Biol. Chem. 33: 686–692.
Ristau, E. and Wagner, F. 1983. Formation of novel anionic trehalose-tetraesters from Rhodococcus erythropolis under growth limiting conditions. Biotechnol. Lett. 5: 95–100.
Itoh, A., Honda, H., Tomita, F. and Suzuki, T. 1971. Rhamnolipids produced by Pseudomonas aeruginosa grown on n-paraffin J. Antibiotics 24: 855–859.
Kakinuma, A., Ouchida, A., Shima, T., Sugino, H., Isono, M., Tamura, G. and Arima, K. 1969. Confirmation of the structure of surfactin by mass spectrometry Agr. Biol. Chem. 33: 1669–1671.
Kretschmer, A., Bock, H. and Wagner, F. 1982. Chemical and physical characterization of interfacial-active lipids from Rhodococcus erythropolis grown on n-alkanes Appl. Environ. Microbiol. 44: 864–870.
Akit, J., Cooper, D.G., Manninen, K.I. and Zajic, J.E. 1981. Investigation of potential biosurfactant production among phytopathogenic Corynebacteria and related soil microbes Current Microbiol. 6: 145–150.
Goursad, J.-C. 1989. Production of a biosurfactant by Bacillus lickeniformis JF-2. Thesis, The University of Texas, Austin, TX.
Syldatk, C. and Wagner, F. 1987. Production of Biosurfactants. In: Biosurfactants and Biotechnology. Kosaric, N., Cairns, W. L., and Gary, N. C. C. (Eds.). Marcel Decker, Inc., NY.
Greiner, M. and Winkelman, G. 1987. Fermentation and isolation of herbicolin A, a peptide produced by Erwinia herbicola strain A111. Appl. Microbiol. Biotechnol. 34: 565–569.
Kretschmer, A. and Wagner, F. 1983. Characterization of biosynthetic intermediates of trehalose dicorynomycolates from Rhodococcus erythropolis grown on n-alkanes. Biochim. Biophys. Acta. 753: 306–313.
Suzuki, T., Tanaka, H. and Itoh, S. 1974. Sucrose lipids of Arthrobacteria, Corynebacteriaand Nocardiagrown on sucrose Agr. Biol. Chem. 38: 557–563.
Itoh, S. and Suzuki, T. 1974. Fructose-lipids of Arthrobacter, Corynebacteria, Nocardia and Mycobacteria grown on fructose. Agr. Biol. Chem. 38: 1443–1449.
Boulton, C.A. and Ratledge, C. 1987. Biosynthesis of lipid precursors to surfactant production. In: Biosurfactants and Biotechnology. Kosaric, N., Cairns, W. L., and Gary, N. C. C. (Eds.). Marcel Decker Inc., NY.
Vater, J., 1984. Lipopeptides, an interesting class of microbial secondary metabolites. Progr. Colloid Polymer Sci. 72: 12–18.
Kleinkauf, H. and von Döhren, H. Biosynthesis of peptide antibiotics. Ann. Rev. Microbiol. 41: 259–289.
Nakano, M.M. and Zuber, P. 1990. Molecular biology of antibiotic production in Bacillus CRC Critical Reviews in Biotechnol. 10: 223–240.
Nakano, M.M., Corbell, N. and Zuber, P. 1991. Isolation and characterization of sfp: a gene required for the production of the lipopeptide biosurfactant, surfactin, in Bacillus subtilis . Mol. Gen. Genet. In press.
Kluge, B., Vater, J., Salnikow, J. and Eckart, K. 1988. Studies on the biosynthesis of surfactin, a lipopeptide antibiotic from Bacillus subtilis ATCC 21332. FEES Lett. 231: 107–110.
Ullrich, C., Kluge, B., Palacz, Z. and Vater, J. 1991. Cell-free biosynthesis of surfactin, a cyclic lipopeptide produced by Bacillus subtilis . Biochemistry 30: 6503–6508.
Leahy, J.G. and Colwell, R.R. 1990. Microbial degradation of hydrocarbons in the environment Microbiol. Rev. 54: 305–315.
Broderick, L.S. and Cooney, J.J. 1982. Emulsification of hydrocarbons by bacteria from freshwater ecosystems. Dev. Ind. Microbiol. 23: 425–434.
Oberbremer, A., Müller-Hurtig, R. and Wagner, F. 1990. Effect of the addition of microbial surfactants on hydrocarbon degradation in a soil population in a stirred reactor Appl. Microbiol. Biotechnol. 32: 485–489.
Harvey, S., Elashvili, I., Valdes, J.J., Kamely, D. and Chakrabarty, A.M. 1990. Enhanced removal of Exxon Valdez spilled oil from Alaskan gravel by a microbial surfactant Bio/Technol. 8: 228–230.
Kaeppeli, O. and Fiechter, H. 1976. The mode of interaction between the substrate and cell surface of the hydrocarbon utilizing yeast Candida tropicalis . Biotechnol. Bioeng. 18: 967–974.
Kaeppeli, O. and Fiechter, A. 1977. Component from the cell surface of the hydrocarbon-utilization yeast Candida tropicalis with possible relation to hydrocarbon transport. J. Bacteriol. 131: 917–921.
Bar-Ness, R., Avrahamy, N., Matsuyama, T. and Rosenberg, M. 1988. Increased cell surface hydrophobicity of a Serratia marcescens NS 38 mutant lacking wetting activity J. Bacteriol. 170: 4361–4364.
Kochi, M., Weiss, D.W., Pugh, L.H. and Groupe, V.,1951. Viscosin, a new antibiotic Bacteriological Proc. 51: 29–30.
Bernheimer, A.W. and Avigad, L.S. 1970. Nature and properties of a cytolytic agent produced by Bacillus subtilis . J. Gen. Microbiol. 61: 361–369.
Kurioka, S. and Liu, P.V. 1967. Effect of the hemolysin of Pseudomonas aeruginosa on phophatides and on phospholipase c activity. J. Bacteriol. 93: 670–674.
Bailey, J.E. 1991. Towards a science of metabolic engineering Science 252: 1668–1675.
Stephanopoulos, G. and Valine, J.J. 1991. Network rigidity and metabolic engineering in metabolite overproduction. Science 252: 1675–1681.
Koch, A.K., Reiser, J., Käppeli, O. and Fiechter, A. 1988. Genetic construction of lactose-utilizing strains of Pseudomonas aeruginosa and their application in biosurfactant production Bio/Technology 6: 1335–1339.
Matsuyama, T., Sogawa, M. and Yano, I. 1991. Direct colony thin-layer chromatography and rapid characterization of Serratia marcescens mutants defective in production of wetting agents. Appl. Environ. Microbiol. 53: 1186–1188.
Koch, A.K., Käppeli, O., Fiechter, A. and Reiser, K. 1991. Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants. J. Bacteriol. 173: 4212–4219.
Mulligan, C.N., Cooper, D.G. and Neufeld, R.J. 1984. Selection of microbes producing biosurfactants in media without hydrocarbons. J. Ferment. Technol. 62: 311–314.
Mulligan, C.N., Chow, T.Y.K. and Gibbs, B. 1989. Enhanced biosurfactant production by a mutant Bacillus subtilis strain Appl. Microbiol. Biotechnol. 31: 486–489.
Nakano, M.M. and Zuber, P. 1988. Identification of a genetic locus required for biosynthesis of the lipopeptide antibiotic surfactin in Bacillus subtilis . J. Bacteriol. 170: 5662–5668.
Nakano, M.M., Magnuson, R., Myers, A., Curry, J., Grossman, A.D. and Zuber, P. 1991. srfA is an operon required for surfactin production, competence development and efficient sporulation in Bacillus subtilis. J. Bacteriol. 173: 1770–1778.
Crossman, A. 1991. Integration of developmental signals and the initiation of sporulation in B subtilis. Cell. 65: 5–8.
Nakano, M.M. and Zuber, P. 1991. Transcription initiation region of the srfA operon, which is controlled by the comP-comA signal transduction system in Bacillus subtilis . J. Bacteriol. In press.
Guerra-Santos, L., Käppeli, O. and Fiechter, A. 1984. Pseudomonas aeruginosa biosurfactant production in continuous culture with glucose as carbon source. Appl. Environ. Microbiol. 48: 301–305.
Suzuki, T., Tanaka, K. and Kinoshita, S. 1969. The extracellular accumulation of trehalose and glucose by bacteria grown on n-alkanes Agr. Biol. Chem. 33: 190–195.
Rubinowitz, C., Gutnick, D.L. and Rosenberg, E. 1982. Emulsan production by Acinetobacter calcoaceticus in the presence of chloram-phenicol J. Bacteriol. 152: 126–132.
Rosenberg, E., Zuckerberg, A., Rubinovitz, C. and Gutnick, D.L. 1979. Emulsifier of ArthrobacterRAG-1: Isolation and emulsifying properties Appl. Environm. Microbiol. 37: 402–408.
Iguchi, T., Takeda, I. and Ohsawa, H. 1969. Emulsifying factor of hydrocarbon produced by a hydrocarbon-assimilating yeast Agr. Biol. Chem. 33: 1657–1658.
Mulligan, C.N. and Gibbs, B.F. 1990. Recovery of biosurfactants by ultrafiltration. J. Chem. Tech. Biotechnol. 47: 23–29.
Kachholtz, T. and Schlingmann, M. 1987. Possible food and agricultural applications of microbial surfactants: An assessment. In: Biosurfactants and Biotechnology. Kosaric, N., Cairns, W. L., and Gary, N. C. C. (Eds.). Marcel Decker, Inc., NY.
Horowitz, S. and Currie, J.K. 1990. Novel dispersants of silicon and aluminum nitride J. Dispersion Sci. Technol. 11: 637–659.
Brown, M.J., Robinson, J.P. and Springham, D.G. 1986. Microbial enhanced oil recovery: progress and prospects CRC Crit. Rev. in Biotechnol. 3: 159–197.
Sarkar, A.K., Goursaud, J.-C., Sharma, M.M. and Georgiou, G. 1990. A critical evaluation of MEOR processes In situ 13: 207–238.
Oberbremer, A., Muller-Hurtig, R. and Wagner, F. 1990. Effect of the addition of microbial surfactants on hydrocarbon degradation in a soil population in a stirred reactor. Appl. Microbiol. Biotechnol. 32: 485–489.
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Georgiou, G., Lin, SC. & Sharma, M. Surface–Active Compounds from Microorganisms. Nat Biotechnol 10, 60–65 (1992). https://doi.org/10.1038/nbt0192-60
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DOI: https://doi.org/10.1038/nbt0192-60
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