Summary
Ultraviolet mutation of Bacillus subtilis ATCC 21332 yielded a stable mutant that produced over three times more of the biosurfactant, surfactin, than the parent strain. By protoplast fusing the mutant (Suf-1) with the marker strain, B. subtilis BGSC strain IA28, the mutation was located between argC4 and hisA1 on the genetic map.
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Mulligan, C.N., Chow, T.Y.K. & Gibbs, B.F. Enhanced biosurfactant production by a mutant Bacillus subtilis strain. Appl Microbiol Biotechnol 31, 486–489 (1989). https://doi.org/10.1007/BF00270781
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DOI: https://doi.org/10.1007/BF00270781