Skip to main content
Log in

Effect of the addition of microbial surfactants on hydrocarbon degradation in a soil population in a stirred reactor

  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

The hydrocarbon degradation rate could be doubled by the addition of sophorose lipids as biosurfactants in a model system containing 10% soil and a 1.35% hydrocarbon mixture of tetradecane, pentadecane, hexadecene, 1,2,4-trimethylcyclohexane, pristane (2,6,10,14-tetramethylpentadecane) phenyldecane and naphthalene suspended in mineral salts medium. The adaptation phases for two degradation phases were shortened, and the extent of degradation and final biomass were increased. The added biosurfactants were degraded after they had facilitated degradation of all hydrocarbon components.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Atlas RM, Bartha R (1973) Effects of some commercial oil herders, dispersants and bacterial inocula on Biodegradation of oil in seawater. In: Ahearn DG, Meyers SP (eds) The microbial degradation of oil pollutants. Lousiana State University publ. no. LSU-SG-73-01, Baton Rouge, pp 283–289

  • Bernern K-H van (1984) Experimentelle Untersuchungen zur Wirkung von Rohöl und Rohöl/Tensid-Gemischen im Ökosystem Wattenmeer. II. Eindringverhalten und Persistenz von Rohölkohlenwasserstoffen in Sedimenten nach experimenteller Kontamination. Senckenb Marit 16:13–23

    Google Scholar 

  • Bossert I, Bartha R (1984) The fate of petroleum in soil ecosystems. In: Atlas RM (ed) Petroleum microbiology. Macmillan, New York, pp 435–473

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal Biochem 72:248–254

    Google Scholar 

  • Cooney J (1984) The fate of petroleum pollutants in freshwater ecosystems. In: Atlas RM (ed) Petroleum microbiology. Macmillan, New York, pp 399–433

    Google Scholar 

  • Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung (1981) 9. Lieferung H 41, Verlag Chemie, Weinheim

  • Hisatsuka K-I, Nakahara T, Sano N, Yamada K (1971) Formation of rhamnolipid byPseudomonas aeruginosa and its function in hydrocarbon fermentation. Agric Biol Chem 35:686–692

    Google Scholar 

  • Hodge JD, Hofreiter BT (1962) Determination of reducing sugars and carbohydrates. In: Whistler RL, BeMiller IN (eds) Methods in carbohydrate chemistry, vol 1: Academic Press, New York, pp 380–394

    Google Scholar 

  • Lang S, Wagner F (1987) Structure and properties of biosurfactants. In: Kosaric N, Cairns WL, Gray NCC (eds) Biosurfactants and biotechnology. Dekker, New York, pp 21–45

    Google Scholar 

  • Mattei G, Bertrand J-C (1985) Production of biosurfactants by a mixed bacteria population grown in continuous culture on crude oil. Biotechnol Lett 7:217–222

    Google Scholar 

  • Mattei G, Rambeloarisoa E, Giusti G, Rontani JF, Bertrand JC (1986) Fermentation procedure of a crude oil in continuous culture on seawater. Appl Microbiol Biotechnol 23:302–304

    Google Scholar 

  • Näveke R, Tepper KP (1982) Einführung in die mikrobiologischen Arbeitsmethoden. Fischer, Stuttgart

    Google Scholar 

  • Oberbremer A, Müller-Hurtig R (1989) Aerobic stepwise hydrocarbon degradation and formation of biosurfactants by an original soil population in a stirred reactor. Appl Microbiol Biotechnol 31:582–586

    Google Scholar 

  • Shailubhai K (1986) Treatment of petreoleum industry oil sludge in soil. Trends Biotechnol 4:200–206

    Google Scholar 

  • Syldatk C, Wagner F (1987) Production of biosurfactants. In: Koraric N, Cairns WL, Gray NCC (eds) Biosurfactants and biotechnology. Dekker, New York, pp 89–120

    Google Scholar 

  • Zajic JE, Seffens W (1984) Biosurfactants. CRC Crit Rev Microbiol 5:87–107

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oberbremer, A., Müller-Hurtig, R. & Wagner, F. Effect of the addition of microbial surfactants on hydrocarbon degradation in a soil population in a stirred reactor. Appl Microbiol Biotechnol 32, 485–489 (1990). https://doi.org/10.1007/BF00903788

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00903788

Keywords

Navigation