Abstract
The amplified fragment length polymorphism (AFLP) technique, employing two different rare cutters, EcoRI and MluI in combination with the frequent cutter MseI, was used to assess genetic diversity and relationships among 21 closely related cultivated Southern African peanut genotypes. A dendrogram was constructed using Jaccard's coefficient and the UPGMA clustering method. Low levels of polymorphism (on average 2.78%) were detected. Results indicated that both EcoRI/MseI and MluI/MseIAFLP enzyme combinations efficiently detected polymorphism within closely related cultivated peanut, although the EcoRI/MseI enzyme combination detected more fragments per primer combination (on average 67.8) as opposed to29.7 by the MluI/MseI enzyme combination. All 21 genotypes could be uniquely distinguished from each other with a minimum of three MluI/MseI primer combinations. Genetic data correlated well with known species and pedigree data, dividing the 21 genotypes into two main groups corresponding to the two subspecies of Arachis hypogaea namely fastigiata and hypogaea. Divisions within the two main groups correlated with botanical types and pedigree data. This is the first report where MluI/MseI primer combinations were used on cultivated peanut and also the first successful detection of polymorphisms between closely related cultivated peanut genotypes worldwide.
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Bhagwat, A., T.G. Krishna & C.R. Bhatia, 1997. RAPD analysis of induced mutants of groundnut (Arachis hypogaea L.). J Genet 76: 201-208.
Blears, M.J., S.A. De Grandis, H. Lee & J.T. Trevors, 1998. Amplified fragment length polymorphism (AFLP): a review of the procedure and its applications. J Ind Microbiol Biotechnol 21: 99-114.
Burow, M.D., C.E. Simpson, A.H. Paterson & J.L. Starr, 1996. Identification of peanut (Arachis hypogaea L.) RAPD markers diagnostic of root-knot nematode (Meloidogyne arenaria (Neal) Chitwood) resistance. Mol Breed 2: 369-379.
Cilliers, A.J. & C.J. Swanevelder, 2003. Status of the South African germplasm collection of groundnut, Arachis hypogaea. SA J Plant Soil 20: 93-95.
Divaret, I., Margalé, E. & G. Thomas, 1999. RAPD markers on seed bulks efficiently assesses the genetic diversity of a Brassica oleracea L. collection. Theor Appl Genet 98: 1029-1035.
Garcia, G.M., H.T. Stalker & G. Kochert, 1994. Introgression analysis of an interspecific hybrid population in peanuts (Arachis hypogaea L.) using RFLP and RAPD markers. Genome 38: 166-176.
Gilbert, J.E., R.V. Lewis, M.J. Wilkinson & P.D.S. Caligari, 1999. Developing an appropriate strategy to assess genetic variability in plant germplasm collections. Theor Appl Genet 98: 1125-1131.
Grieshammer, U. & J.C. Wynne, 1990. Isozyme variability in mature seeds of U.S. peanut cultivars and collections. Peanut Sci 18: 72-75.
Halward, T., H.T. Stalker & G. Kochert, 1993. Development of an RFLP linkage map in diploid peanut species. Theor Appl Genet 87: 379-384.
Halward, T., H.T. Stalker & G. Kochert, 1994. RFLP map of peanut. In: R.L. Phillips & I.K. Vasil (Eds.), DNA-based Markers in Plants, pp. 246-260. Kluwer Academic Publishers, London, UK.
Halward, T.M., H.T. Stalker, E.A. LaRue & G. Kochert, 1991. Genetic variation detectable with molecular markers among unadapted germplasm resources of cultivated peanut and related wild species. Genome 34: 1013-1020.
Halward, T., T. Stalker, E. LaRue & G. Kochert, 1992. Use of singleprimer DNA amplifications in genetic studies of peanut (Arachis hypogaea L.). Plant Mol Biol 18: 315-325.
He, G. & C.S. Prakash, 1997. Identification of polymorphic DNA markers in cultivated peanut (Arachis hypogaea L.). Euphytica 97: 143-149.
Hopkins, M.S., A.M. Casa, T. Wang, S.E. Mitchell, R.E. Dean, G.D. Kochert & S. Kresovich, 1999. Discovery and characterization of polymorphic simple sequence repeats (SSRs) in peanut. Crop Sci 39: 1243-1247.
Jaccard, P., 1908. Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44: 223-270.
Kochert, G., 1995. Molecular mapping and use of molecular markers. Proc Am Peanut Res Edu Soc, Inc 27: 18.
Kochert, G., T. Halward, W.D. Branch & C.E. Simpson, 1991. RFLP variability in peanut (Arachis hypogaea L.) cultivars and wild species. Theor Appl Genet 81: 565-570.
Krapovickas, A. & W.C. Gregory, 1994. Taxonomia del genera Arachis (Leguminosae). Bonplandia 8: 1-184.
Krapovickas, A. & V.A. Rigoni, 1960. La nomenclatura de las subspecies y variedades de Arachis hypogaea L. Rev de Invest Agric 14: 197-228.
Lacks, G.D. & H.T. Stalker, 1993. Isozyme analysis of Arachis species and interspecific hybrids. Peanut Sci 20: 76-81.
Lanham, P.G., S. Fennell, J.P. Moss & W. Powell, 1992. Detection of polymorphic loci in Arachis germplasm using random amplified polymorphic DNAs. Genome 35: 885-889.
Lu, J. & B. Pickersgill, 1993. Isozyme variation and species relationships in peanut and its wild relatives (Arachis L.-Leguminosae). Theor Appl Genet 85: 550-560.
Paik-Ro, O.G., R.L. Smith & D.A. Knauft, 1992. Restriction fragment length polymorphism evaluation of six peanut species within the Arachis section. Theor Appl Genet 84: 201-208.
Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen & R.W. Allard, 1984. Ribosomal DNA spacer-length polymoprhism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81: 8014-8018.
Savage, G.P. & J.I. Keenan, 1994. The composition and nutritive value of groundnut kernels. In: J. Smartt (Ed.), The groundnut Crop: A Scientific Basis for Improvement, pp. 173-213. Chapman and Hall, London.
Smartt, J. & H.T. Stalker, 1982. Speciation and cytogenetics in Arachis. In: H.E. Patee & C.T. Young (Eds.), Peanut Science and Technology, pp. 21-49. Am Peanut Res Educ Soc, Yoakum, Texas.
Sokal, R.R. & C.D. Michener, 1958. A statistical method for evaluating systematic relationships. Univ Kansas Sci Bull 38: 1409-1438.
Stalker, H.T., T.D. Phillips, J.P. Murphy & T.M. Jones, 1994. Variation of isozyme patterns among Arachis species. Theor Appl Genet 87: 746-755.
Subramanian, V., S. Gurtu, R.C. Nageswara Rae & S.N. Nigam, 2000. Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay. Genome 43: 656-660.
Swanevelder, C.J., 2000. Groundnuts Always Tops. Agricultural Research Council, Potchefstroom.
van der Merwe, P.J.A., 1981. Die Geskiedenis van Grondbone in Suidelike Afrika. Grondbone A.2.
van der Merwe, P.J.A. & L.P. van der Merwe, 1988. Harts: A groundnut cultivar with resistance to black pod rot (Chalara elegans). Appl Plant Sci 2: 82-84.
van der Merwe, P.J.A. & W.J. Vermeulen, 1977. A new groundnut cultivar: Sellie. Agroplantae 9: 71-72.
Vierling, R.A., Z. Xiang, C.P. Joshi, M.L. Gilbert & H.T. Nguyen, 1994. Genetic diversity among elite Sorghum lines revealed by resitriction fragment length polymorphisms and random amplified polymorphic DNAs. Theor Appl Genet 87: 816-820.
Vivek, B.S. & P.W. Simon, 1999. Phylogeny and relationships in Dacus based on restriction fragment length polymorphisms (RFLPs) of the chloroplast and mitochondrial genomes. Euphytica 105: 183-189.
Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Van De Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper & M. Zabeau, 1995. AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 21: 4407-4414.
Wynne, J.C. & T.M. Halward, 1989. Cytogenetics and genetics of Arachis. Crit Rev Plant Sci 8: 189-220.
Young, N.D., N.F. Weeden & G. Kochert, 1996. Genome mapping in legumes (Fam. Fabaceae). In: A.H. Paterson (Ed.), Genome Mapping in Plants, pp. 211-227. R.G. Landes Co., Austin, Texas.
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Herselman, L. Genetic variation among Southern African cultivated peanut (Arachis hypogaea L.) genotypes as revealed by AFLP analysis. Euphytica 133, 319–327 (2003). https://doi.org/10.1023/A:1025769212187
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DOI: https://doi.org/10.1023/A:1025769212187