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Genetic diversity and relatedness among ornamental purslane (Portulaca L.) accessions unraveled by SRAP markers

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Abstract

Ornamental purslanes (Portulaca L.) are a popular annual bedding and container plant for landscaping. Little information is available concerning the genetic characterization of ornamental purslane resources thus far. The purpose of this study was to investigate the genetic diversity and relationships present in a collection of ornamental purslanes from Portulaca umbraticola and P. grandiflora cultivated in China, using sequence-related amplified polymorphism (SRAP) markers. The genotyping showed that 16 SRAP primer combinations totally produced 261 informative fragments and averaged 16.31 per primer combination. The major allele frequency and Nei’s gene diversity was calculated at 0.78 and 0.31 across the loci, indicative of a moderate low diversity. Both unweighted pair group method with arithmetic average (UPGMA) clustering and a Bayesian-based approach apparently assigned the whole accessions into two sub-groups: P. umbraticola and P. grandiflora, well concordant with the botanical classification and flower type. The findings provide a brandnew understanding of genetic diversity and population structure present in ornamental purslane, and benefit a sound design of breeding programs in future.

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Acknowledgements

The present research was partly financed by Top-notch Academic Programs Project of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. Department of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, Jiangsu Province, China

    Sizhen Jia, Zhiming Yan, Yuanhua Wang, Yue Wei & Zhenqiang Xie

  2. Jiangsu Engineering and Technology Center for Modern Horticulture, Jurong, 212400, Jiangsu Province, China

    Sizhen Jia, Zhiming Yan, Yuanhua Wang, Yue Wei & Zhenqiang Xie

  3. College of Horticulture, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095, Jiangsu Province, China

    Fei Zhang

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  1. Sizhen Jia
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  2. Zhiming Yan
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  3. Yuanhua Wang
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  4. Yue Wei
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  5. Zhenqiang Xie
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Correspondence to Fei Zhang.

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Jia, S., Yan, Z., Wang, Y. et al. Genetic diversity and relatedness among ornamental purslane (Portulaca L.) accessions unraveled by SRAP markers. 3 Biotech 7, 241 (2017). https://doi.org/10.1007/s13205-017-0881-8

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