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Germination and net in vitro growth of peach, almond and peach-almond hybrid embryos in response to mannitol inclusion in the nutrient medium

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Abstract

Nine Prunus accessions were evaluated for germination and plantlet growth in an in vitro osmotic screening test using mannitol as an osmoticum. Embryos from diverse peaches, almonds and peach-almond hybrids were cultured in Woody Plant Medium, and in this same medium modified with the inclusion of 350 mM mannitol. Embryos were stratified in vitro for 60 days, induced to germinate for a two week period and then allowed to develop and grow for another 20 days prior to harvest. Fresh weights of both roots and shoots as well as percent germination were recorded at harvest. The main effects of nutrient medium, germplasm type (peach, peach-almond, almond) and specific Prunus accession were all highly significant (P = 0.01) with regard to fresh weight of roots and shoots. Embryo germination was affected significantly by the inclusion of mannitol in the nutrient medium and by the particular germplasm type (P=0.01 and P=0.05, respectively). Significant interactions of nutrient medium × Prunus accession and nutrient medium × germplasm type were also detected for both root and shoot fresh weights. Almond germplasm cultured in Woody Plant Medium with 350 mM mannitol produced significantly more roots and shoots than either peaches or peach-almond hybrids grown in the same medium. Peach-almond hybrid embryos were observed to germinate at a significantly higher frequency than peach embryos when averaged across both media. Results obtained in this study indicate a wide range of average plantlet fresh weight relative to the specific germplasm challenged in the osmotic screen.

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References

  • Atokple, I.D.K., B.B. Singh & A.M. Emechebe, 1993. Independent inheritance of Striga and Alectra resistance in cowpea genotype B301. Crop Science 33(4): 714-715.

    Article  Google Scholar 

  • Blum, A., 1989. Breeding methods for drought resistance. In: Hamlyn G. Jones, T.J. Flowers & M.B. Jones (Eds), Plants Under Stress. Cambridge University Press, Cambridge.

    Google Scholar 

  • Brooks, R.M. & H.P. Olmo, 1972. Register of New Fruit and Nut Varieties. (Second Edition). University of California Press. Berkeley, California.

    Google Scholar 

  • Day, L.H., 1953. Rootstocks for Stone Fruits. University of California, California Agricultural Experiment Station Extension Bulletin No. 736.

  • Ehdaie, B., A.E. Hall, G.D. Farquhar, H.T. Nguyen & J.G. Waines, 1991. Water-use efficiency and carbon isotope discrimination in wheat. Crop Science 31(5): 1282-1288.

    Article  Google Scholar 

  • Ehlenfeldt, M.K., 1994. The genetic composition and tetrasomic inbreeding coefficients of highbush blueberry cultivars. Hort Science 29(11): 1342-1345.

    Google Scholar 

  • Erb, W.A., A.D. Draper & H.J. Swartz, 1988. Methods of screening blueberry seedling populations for drought resistance. Hort Science 23(2): 312-314.

    Google Scholar 

  • Felipe, A.J., 1989. Frutales de hueso. In: Ediciones Técnicas Europeas s.a. (Eds), Patrones para Frutales de Pepita y Hueso, pp. 64-138. T.G. Alfadir, S.A. Barcelona, España.

    Google Scholar 

  • Garrett, C.M.E., 1986. Influence of rootstock on the susceptibility of sweet cherry scions to bacterial canker, caused by Pseudomonas syringaepvs morsprunorumand syringae. Plant Pathology 35(1): 114-119.

    Google Scholar 

  • Hashmi, G.P., F.A. Hammerschlag, R.N. Huettel & L.R. Krusherg. 1995. Growth, development and response of peach somaclones to the root-knot nematode, Meloidogyne incognita. Journal of the American Society of Horticultural Science 120(6): 932-937.

    Google Scholar 

  • Hsissou, D. & J. Bouharmont, 1994. In vitroselection and characterization of drought-tolerant plants of duram wheat (Triticum durumDesf). Agronomie 2: 65-70.

    Google Scholar 

  • Jones, R.W., 1969. Selection of intercompatible almond and rootknot nematode resistant peach rootstocks as parents for production of hybrid rootstock seed. Journal of the American Society of Horticultural Science 94(2): 89-91.

    Google Scholar 

  • Jones, R.W., 1972. Titan, a seed source for F1 almond ×Nemaguard peach hybrids. Fruit Varieties and Horticultural Digest. 26(1): 18-20.

    Google Scholar 

  • Karunaratne, S., S. Santha & A. Kovoor. 1991. An in vitroassay for drought-tolerant coconut germplasm. Euphytica 53: 25-30.

    Article  Google Scholar 

  • Knowles, S.E., G.F. McLaren, P.G. Glucina & P. Alspach, 1994. Performance of "sundrop’ apricot on 23 rootstocks. New Zealand Journal of Crop and Horticultural Science 22: 419-430.

    Article  Google Scholar 

  • Krishna Rao, M., K. Uma Devi & A. Arundhati, 1990. Applications of genic male sterility in plant breeding. Plant Breeding 105: 1- 25.

    Article  Google Scholar 

  • Krizek, D.T., 1985. Methods of inducing water stress in plants. Hort Science 20(6): 1028-1038.

    Google Scholar 

  • Ledbetter, C.A., S. Peterson & D. Palmquist, 1996. In vitroosmotic tolerance of six clonally propagated Prunusaccessions. Journal of Genetics and Breeding 50: 1-6.

    Google Scholar 

  • Lloyd, G. & B. McCowen, 1981. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings of the International Plant Propagation Society 30: 421-427.

    Google Scholar 

  • Lipavská, H. & D. Vreugdenhil, 1996. Uptake of mannitol from the media by in vitrogrown plants. Plant Cell, Tissue and Organ Culture 45: 103-107.

    Article  Google Scholar 

  • Okie, W.R., G.L Reighard, T.G. Beckman, A.P. Nyczepir, C.C. Reilly, E.I. Zehr, W.C. Newall Jr., & D.W. Cain, 1994. Fieldscreening Prunusfor longevity in the southeastern United States. HortScience 29(6): 673-677.

    Google Scholar 

  • Ouendeba, G. Ejeta, G. Hanna, W.W. & A.K. Kumar, 1995. Diversity among African pearl millet landrace populations. Crop Science 35(3): 919-924.

    Article  Google Scholar 

  • Rajashekar, G., D. Palmquist & C.A. Ledbetter, 1995. In vitroscreening procedure for osmotic tolerance in Prunus. Plant Cell, Tissue and Organ Culture 41: 159-164.

    Article  Google Scholar 

  • Ramming, D.W., 1983. Embryo culture. In: Moore JN & J. Janick (Eds), Methods in Fruit Breeding (pp. 136-144). Purdue University Press, West Lafayette, Indiana.

    Google Scholar 

  • Ramming, D.W. & O. Tanner, 1983. ‘Nemared’ peach rootstock. HortScience 18(3): 376.

    Google Scholar 

  • Romera, F.J., E. Alcántara & M.D. de la Guardia, 1991. Characterization of the tolerance to iron chlorosis in different peach rootstocks grown in nutrient solution. I. Effect of bicarbonate and phosphate. Plant and Soil 130: 115-119.

    Article  CAS  Google Scholar 

  • Sapra V.T. & A.O. Anaele, 1991. Screening soybean genotypes for drought and heat tolerance. Journal of Agronomy and Crop Science 167: 96-102.

    Article  Google Scholar 

  • Saranga, Y., D. Zamir, A. Marani & J. Rudich, 1993. Breeding tomatoes for salt tolerance: variations in ion concentrations associated with response to salinity. Journal of the American Society of Horticultural Science 118(3): 405-408.

    Google Scholar 

  • SAS Institute Inc. 1989. SAS/STAT® User's Guide, Version 6. Fourth Edition, Volume 2, Cary, NC: SAS Institute Inc. 846 pp.

    Google Scholar 

  • Sharma, S.B., P. Ashokkumar & D. McDonald, 1991. A greenhouse technique to screen pigeonpea for resistance to Heterodera cajani. Annals of Applied Biology 118: 351-356.

    Article  Google Scholar 

  • Sherman, W.B., P.M. Lyrene & R.H. Sharpe, 1991. Flordaguard peach rootstock. HortScience 26(4): 427-428.

    Google Scholar 

  • Snow, L.M., 1905. Development of root hairs. Botanical Gazette 40: 12-48.

    Article  Google Scholar 

  • Uyemoto, J.K., C.F. Luhn, J.A. Griesbach & J.A. Grant, 1995. Occurrence and control of cherry stem pitting disease. Plant Disease 79(4): 366-368.

    Article  Google Scholar 

  • Winer, B.J., 1971. Statistical principles in experimental design. (Second Edition). McGraw-Hill Book Company, New York, NY.

    Google Scholar 

  • Wright, L.N. & G.L. Jordan, 1970. Artificial selection for seedling drouth tolerance in Boer Lovegrass (Eragrostis curvulaNees) Crop Science 10: 99-102.

    Article  Google Scholar 

  • Yadava, U.L. & S.L. Doud, 1989. Rootstock and scion influence growth, productivity, survival, and short life-related performance of peach trees. Journal of the American Society of Horticultural Science 114 (6): 875-880.

    Google Scholar 

  • Yoshida, M.& S. Kanetsugu, 1981. Breeding of peach rootstocks resistant to root-knot nematode. II Breeding of resistant rootstocks by hybridization. Bulletin of the Fruit Tree Research Station, Ministry of Agriculture, Forestry and Fisheries, Series A No. 8. 31-44.

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Authors and Affiliations

  1. United States Department of Agriculture, Agricultural Research Service, Horticultural Crops Research Laborarory, 2021 S. Peach Ave., Fresno, California, 93727-5951, U.S.A

    Craig A. Ledbetter & Sharon J. Peterson

  2. United States Department of Agriculture, Agricultural Research Service, Ecology of Temperate Desert Rangelands, 920 Valley Road, Reno, Nevada, 89512, U.S.A

    Debra E. Palmquist

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  1. Craig A. Ledbetter
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  2. Debra E. Palmquist
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  3. Sharon J. Peterson
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Ledbetter, C.A., Palmquist, D.E. & Peterson, S.J. Germination and net in vitro growth of peach, almond and peach-almond hybrid embryos in response to mannitol inclusion in the nutrient medium. Euphytica 103, 243–250 (1998). https://doi.org/10.1023/A:1018324624252

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