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Relationship between the concentration of macroelements, their uptake and multiplication of cherry rootstock Gisela 5 in vitro

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Abstract

Sweet cherry rootstock Gisela 5 was micropropagated on Murashige and Skoog (MS) medium, on MS medium containing double-strength macro salts (MS 2×), 1/2 strength (MS 1/2) and 1/4 strength (MS 1/4) with 4.4 μM BA, 0.5 μM NAA, and 0.3 μM GA3. All the analyses and parameters were monitored on day 0, day 20 and day 40 after subculturing. As fresh and dry weight of the explants increased during subculturing, the fresh and dry weight of the media decreased. The pH of the media declined during subculturing following by slow increase on media MS 1/2 and MS 1/4. Gisela 5 showed the best growth and development on MS 2× and MS media with the highest N and P uptake. Growth and multiplication depend on the uptake of these elements from the medium.

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Authors and Affiliations

  1. Fruit and Grape Research Centre, ARI ‘Serbia’, Kralja Petra I/9, 32000, Čačak, Yugoslavia

    Durdina Ružić, Durdina Ružić & Radosav Cerović

  2. The Serbian Academy of Science and Art, Knez Mihailova 35, 11000, Belgrade, Yugoslavia

    Miloje Sarić

  3. The Faculty of Biology, Studentski trg 16, 11000, Belgrade, Yugoslavia

    Ljubinka Ćulafić

Authors
  1. Durdina Ružić
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  2. Miloje Sarić
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  3. Radosav Cerović
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  4. Ljubinka Ćulafić
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Correspondence to Durdina Ružić.

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Ružić, D., Sarić, M., Cerović, R. et al. Relationship between the concentration of macroelements, their uptake and multiplication of cherry rootstock Gisela 5 in vitro . Plant Cell, Tissue and Organ Culture 63, 9–14 (2000). https://doi.org/10.1023/A:1006412901992

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