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Determining the Tolerance of Selected Almond Rootstock Genotypes to Salt Stress Under in vitro Conditions

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Abstract

The research was carried out to determine the salt stress tolerance status of some almond genotypes considered as rootstock candidates under in vitro conditions. The genotypes were cultured in the MS nutrient medium containing 0, 50, 100 and 150 mM NaCl. Apart from NaCl, 1.0 mg/l BAP, 0.01 mg/l IBA, 30 g/l sucrose and 7 g/l agar were added to the nutrients and the pH was adjusted to 5.7. In vitro shoots were incubated for 4 weeks in a climate room with 16‑h light and 24 °C temperature, and then the number of shoots per explant, the proline, chlorophyll, total phenolics, total flavonoids and total protein contents, superoxide dismutase, CAT and APX enzyme activities were evaluated. In parallel with the increase in salt stress level, it was determined that the number of shoots and chlorophyll contents decreased significantly in all genotypes as compared to the control treatment. The proline, total phenolic, total flavonoid and total protein contents and enzyme activities increased significantly with the increase in the salt level. In the study, no significant difference was observed regarding the tolerance status of the genotypes in the MS medium containing 50 mM NaCl. Considering the 100 and 150 mM NaCl applications, it was determined that the genotypes numbered 9, 29, 54, 120, 134, 183, 185, 196 and 241 showed better development and therefore they stood out in terms of salt tolerance as compared to the other genotypes.

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References

  • Ahmed CB, Rouina BB, Boukhris M (2008) Changes in water relations, photosynthetic activity and proline accumulation in one-year-old olive trees (Olea europaea L. cv. Chemlali) in response to NaCl salinity. Acta Physiol Plant 30:553–560. https://doi.org/10.1007/s11738-008-0154-6

    Article  CAS  Google Scholar 

  • Akça Y, Samsunlu E (2012) The effect of salt stress on growth, chlorophyll content, proline and nutrient accumulation, and K/Na ratio in walnut. Pak J Bot 44(5):1513–1520

    Google Scholar 

  • Akçay D, Eşitken A (2017) MM106 anacı ve üzerine aşılı Golden Delicious elma çeşidine tuz stresinin etkileri. Selçuk Tarım Bilimleri Dergisi 3(2):228–232

    Google Scholar 

  • Aono M, Kubo A, Saji H, Tanaka K, Kondo N (1993) Enhanced tolerance to photooxidative stress of transgenic Nicotiana tabacum with high chloroplastic glutathione reductase activity. Plant Cell Physiol 34(1):129–135. https://doi.org/10.1093/oxfordjournals.pcp.a078386

    Article  CAS  Google Scholar 

  • Aras S, Eşitken A (2018) Physiological responses of cherry rootstocks to short term salinity. Erwerbs-Obstbau 60(2):161–164. https://doi.org/10.1007/s10341-017-0350-x

    Article  Google Scholar 

  • Aziz I, Khan MA (2001) Experimental assessment of salinity tolerance of Ceriops tagal seedlings and saplings from the Indus delta, Pakistan. Aquat Bot 70(3):259–268. https://doi.org/10.1016/S0304-3770(01)00160-7

    Article  CAS  Google Scholar 

  • Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195(1):133–140

    Article  CAS  PubMed  Google Scholar 

  • Berli FJ, Moreno D, Piccoli P, Hespanhol-Vıana L, Silva MF, Bressan-Smith R, Cavagnaro JB, Bottini R (2010) Abscisic acid is involved in the response of grape (Vitis vinifera L.) cv. Malbec leaf tissues to ultraviolet‑B radiation by enhancing ultraviolet-absorbing compounds, antioxidant enzymes and membrane sterols. Plant Cell Environ 33(1):1–10. https://doi.org/10.1111/j.1365-3040.2009.02044.x

    Article  CAS  PubMed  Google Scholar 

  • Bolat I, Kaya C, Almaca A, Timucin S (2006) Calcium sulfate improves salinity tolerance in rootstocks of plum. J Plant Nutr 29(3):553–564. https://doi.org/10.1080/01904160500526717

    Article  CAS  Google Scholar 

  • Bolat I, Dikilitas M, Ercisli S, Ikinci A, Tonkaz T (2014) The effect of water stress on some morphological, physiological, and biochemical characteristics and bud success on apple and quince rootstocks. Sci World J. https://doi.org/10.1155/2014/769732

    Article  Google Scholar 

  • Büyük İ, Soydam-Aydın S, Aras S (2012) Bitkilerin stres koşullarına verdiği moleküler cevaplar. Turk Bull Hyg Exp Biol. https://doi.org/10.5505/TurkHijyen.2012.40316

    Article  Google Scholar 

  • Candan N, Tarhan L (2003) The correlation between antioxidant enzyme activities and lipid peroxidation levels in Mentha pulegium organs grown in Ca2+, Mg2+, Cu2+, Zn2+ and Mn2+ stress conditions. Plant Sci 165(4):769–776. https://doi.org/10.1016/S0168-9452(03)00269-3

    Article  CAS  Google Scholar 

  • Caverzan A, Passaia G, Rosa SB, Ribeiro CW, Lazzarotto F, Margis-Pinheiro M (2012) Plant responses to stresses: role of ascorbate peroxidase in the antioxidant protection. Genet Mol Biol 35(4):1011–1019. https://doi.org/10.1590/S1415-47572012000600016

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Çelik A, Eraslan F (2015) Nitrik oksit uygulamasının tuz stresi altında yetiştirilen mısır bitkisinin mineral beslenmesi ve bazı fizyolojik özellikleri üzerine etkisi. Ziraat Fakültesi Dergisi 10(1):55–64

    Google Scholar 

  • Çetin ES, Babalık Z, Göktürk Baydar N (2012) Bazı sofralık üzüm çeşitlerinde tanelerdeki toplam karbonhidrat, fenolik madde, antosiyanin, β‑karoten ve C vitamini içeriklerinin belirlenmesi. In: IV. Ulusal Üzümsü Meyveler Sempozyumu, pp 3–5

    Google Scholar 

  • Channuntapipat C, Sedgley M, Collins G (2003) Micropropagation of almond cultivars Nonpareil and Ne Plus Ultra and the hybrid rootstock Titan x Nemaguard. Sci Hortic 98:473–484. https://doi.org/10.1016/S0304-4238(03)00067-0

    Article  CAS  Google Scholar 

  • Constantine NG, Stanley KR (1977) Superoxide dismutases. Plant Physiol 59(309):e314

    Google Scholar 

  • Çulha Ş, Çakırlar H (2011) Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi 11(2):11–34

    Google Scholar 

  • Dal Bİ, Yazıcı K, Baktır İ (2001) Tuz stresi ve bahçe bitkileri üzerindeki etkileri. Derim 18(3):122–131

    Google Scholar 

  • Daşgan HY, Koç S, Ekici B, Aktaş H, Abak K (2006) Bazı fasulye ve börülce genotiplerinin tuz stresine tepkileri. Alatarım 5(1):23–31

    Google Scholar 

  • Dat JS, Vandenabeele E, Vranova M, Van Montagu D, Inze F, Breusegem FV (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795

    Article  CAS  PubMed  Google Scholar 

  • De Britto AJ, Gracelin DHS, Sebastian SR (2011) Antibacterial activity of a few medicinal plants against Xanthomonas campestris and Aeromonas hydrophila. J Biopestic 4(1):57

    Google Scholar 

  • Demiral T, Türkan I (2006) Exogenous glycinebetaine affects growth and proline accumulation and retards senescence in two rice cultivars under NaCl stress. Environ Exp Bot 56(1):72–79. https://doi.org/10.1016/j.envexpbot.2005.01.005

    Article  CAS  Google Scholar 

  • Doğru A, Canavar S (2020) Bitkilerde tuz toleransının fizyolojik ve biyokimyasal bileşenleri. Akademik Platf Mühendislik Ve Fen Bilimleri Dergisi 8(1):155–174. https://doi.org/10.21541/apjes.541620

    Article  Google Scholar 

  • Dölarslan M, Gül E (2012) Toprak bitki ilişkileri açısından tuzluluk. Türk Bilimsel Derlemeler Dergisi 5(2):56–59

    Google Scholar 

  • Edrewa A (2005) Generation and scavenging of reactive oxygen species in chloroplasts: a submolecular approaches. Agric Ecosyst Environ 106:119–133. https://doi.org/10.1016/j.agee.2004.10.022

    Article  CAS  Google Scholar 

  • Ertürk U, Sivritepe N, Yerlikaya C, Bor M, Ozdemir F, Turkan I (2007) Responses of the cherry rootstock to salinity invitro. Biol Plant 51(3):597–600

    Article  Google Scholar 

  • Feng X, Chen L, Lei N, Wang S, Xu X, Zhou G, Li Z (2017) Emulsifying properties of oxidatively stressed myofibrillar protein emulsion gels prepared with (−)-epigallocatechin-3-gallate and NaCl. J Agric Food Chem 65(13):2816–2826. https://doi.org/10.1021/acs.jafc.6b05517

    Article  CAS  PubMed  Google Scholar 

  • Fu M, Li C, Ma F (2013) Physiological responses and tolerance to nacl stress in different biotypes of malus prunifolia. Euphytica 189:101–109

    Article  CAS  Google Scholar 

  • Gadallah MAA (1999) Effects of proline and glycinebetaine on Vicia faba responses to salt stress. Biol Plant 42(2):249–257

    Article  CAS  Google Scholar 

  • Gupta B, Huang B (2014) Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. Int J Genomics. https://doi.org/10.1155/2014/701596

    Article  PubMed  PubMed Central  Google Scholar 

  • Halliwell B (2008) Are polyphenols antioxidants or pro-oxidants? What do we learn from cell culture and in vivo studies? Arch Biochem Biophys 476(2):107–112. https://doi.org/10.1016/j.abb.2008.01.028t

    Article  CAS  PubMed  Google Scholar 

  • Hartree EF (1972) Determination of protein: a modification of the lowry method that gives a linear photometric response. Anal Biochem 48(2):422–427. https://doi.org/10.1016/0003-2697(72)90094-2

    Article  CAS  PubMed  Google Scholar 

  • Hatami E, Shokouhian AA, Ghanbari AR, Naseri LA (2018) Alleviating salt stress in almond rootstocks using of humic acid. Sci Hortic 237:296–302. https://doi.org/10.1016/j.scienta.2018.03.034

    Article  CAS  Google Scholar 

  • Inskeep WP, Bloom PR (1985) Extinction coefficients of chlorophyll a and B in n,n-dimethylformamide and 80 % acetone. Plant Physiol 77(2):483–485. https://doi.org/10.1104/pp.77.2.483

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kafi M, Stewart WS, Borland AM (2003) Carbohydrate and proline contents in leaves, roots, and apices of salt-tolerant and salt-sensitive wheat cultivars1. Russ J Plant Physiol 50(2):155–162

    Article  CAS  Google Scholar 

  • Köşkeroğlu S (2006). Tuz ve stresi altındaki mısır (Zea Mays L.) bitkisinde prolin birikim düzeyleri ve stres parametrelerinin araştırılması. Yüksek Lisans Tezi, Muğla Üniversitesi, Fen Bilimleri Enstitüsü.

  • Li W, Chen M, Wang E, Hu L, Hawkesford MJ et al (2016) Genome-wide analysis of autophagyassociated genes in foxtail millet (Setaria italica L.) and characterization of the function of SiATG8a in conferring tolerance to nitrogen starvation in rice. BMC Genomics 17(1):797

    Article  PubMed  PubMed Central  Google Scholar 

  • Liu B, Li M, Cheng L, Liang D, Zou Y, Ma F (2012) Influence of rootstock on antioxidant system in leaves and roots of young apple trees in response to drought stress. Plant Growth Regul 67(3):247–256

    Article  CAS  Google Scholar 

  • Mashayekhi M, Amiri ME, Habibi F (2015) Study of the biochemical responses and enzymatic activity of GF677 (peach and almond hybrid) rootstock to in vitro salinity stress. J Hortic Sci 29(2):207–2015

    Google Scholar 

  • Meloni DA, Oliva MA, Martinez CA, Cambraia J (2003) Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot 49(1):69–76. https://doi.org/10.1016/S0098-8472(02)00058-8

    Article  CAS  Google Scholar 

  • Moore JN, Janick J (1996) Fruit breeding: tree and tropical fruits; 2. Vine and small fruits; 3. Nuts. John Wiley & Sons, Oxford

    Google Scholar 

  • Naik GR, Joshi GV (1983) Ineffectual role of proline metabolism in salt-stressed sugarcane leaves. Proc Plant Sci 92(3):265–269

    Article  CAS  Google Scholar 

  • Najafian SH, Rahemi M, Tavallali V (2008) Effect of salinity on tolerance of two bitter almond rootstock. American Eurasian Journal of Agricultural and Environmental Sciences 3(2):264–268

    Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22(5):867–880. https://doi.org/10.1093/oxfordjournals.pcp.a076232

    Article  CAS  Google Scholar 

  • Okubo M, Sakuratani T (2000) Effects of sodium chloride on survival and stem elongation of two Asian pear rootstock seedlings. Sci Hortic 85(1):85–90. https://doi.org/10.1016/S0304-4238(99)00141-7

    Article  CAS  Google Scholar 

  • Oluk EA, Sami O (2007) Yüksek öğretim öğrencilerinin sera etkisi, küresel isınma ve iklim değişikliği algılarının analizi. Dokuz Eylül Üniversitesi Buca Eğitim Fakültesi Dergisi 22:45–53.

    Google Scholar 

  • Öztürk NZ (2015) Bitkilerin kuraklık stresine tepkilerinde bilinenler ve yeni yaklaşımlar. Turk J Agric Sci Technol 3(5):307–315. https://doi.org/10.24925/turjaf.v3i5.307-315.307

    Article  Google Scholar 

  • Pareek A, Singla SL, Grover A (1997) Salt responsive proteins/genes in crop plants. Oxford, IBH, New Delhi

    Google Scholar 

  • Paschke M, Bernasconi G, Schmid B (2005) Effects of inbreeding and pollen donor provenance and diversity on offspring performance under environmental stress in the rare plant Cochlearia bavarica. Basic Appl Ecol 6(4):325–338. https://doi.org/10.1016/j.baae.2005.02.005

    Article  Google Scholar 

  • Ramanjulu S, Sudhakar C (2001) Alleviation of NaCl salinity stress by calcium is partly related to the increased proline accumulation in mulberry (Morus alba L.) callus. J Plant Biol 28:203–206

    Google Scholar 

  • Ranjbarfordoei A, Samson R, Van Damme P (2006) Chlorophyll fluorescence performance of sweet almond [Prunus dulcis (Miller) D. Webb] in response to salinity stress induced by NaCl. Photosynt 44(4):513–522

    Article  CAS  Google Scholar 

  • Rebey IB, Bourgou S, Rahali FZ, Msaada K, Ksouri R, Marzouk B (2017) Relation between salt tolerance and biochemical changes in cumin (Cuminum cyminum L.) seeds. J Food Drug Anal 25(2):391–402. https://doi.org/10.1016/j.jfda.2016.10.001

    Article  CAS  PubMed  Google Scholar 

  • Rezazadeh S, Ilkaee M, Aghayari F, Paknejad F, Rezaee M (2019) The physiological and biochemical responses of directly seeded and transplanted maize (Zea mays L.) supplied with plant growth-promoting rhizobacteria (PGPR) under water stress. Iran J Plant Physiol 10(1):3009–3021. https://doi.org/10.22034/IJPP.2019.670787

    Article  Google Scholar 

  • Scandalios JG (1996) Oxidative stress and moleculer biologoy of antioxidant defenses. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  • Shalaby EE, Epstein E, Qualset CO (1993) Variation in salt tolerance among some wheat and triticale genotypes. J Agron Crop Sci 171(5):298–304. https://doi.org/10.1111/j.1439-037X.1993.tb00144.x

    Article  Google Scholar 

  • Shibli RA, Shatnawi MA, Swaidat IQ (2003) Growth, osmotic adjustment, and nutrient acquisition of bitter almond under induced sodium chloride salinity invitro. Commun Soil Sci Plant Anal 34(13–14):1969–1979. https://doi.org/10.1081/CSS-120023231

    Article  CAS  Google Scholar 

  • Shiyab SM, Shibli RA, Mohammad MM (2003) Influence of sodium chloride salt stress on growth and nutrient acquisition of sour orange invitro. J Plant Nutr 26(5):985–996. https://doi.org/10.1081/PLN-120020070

    Article  CAS  Google Scholar 

  • Sibley JL, Eakes DJ, Gilliam CH, Keever GJ, Dozier WA, Himelrick D (1996) Foliar SPAD-502 meter values, nitrogen levels, and extractable chlorophyll for red maple selections. HortScience 31(3):468–470. https://doi.org/10.21273/HORTSCI.31.3.468

    Article  Google Scholar 

  • Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16(3):144–158

    CAS  Google Scholar 

  • Sivritepe N, Erturk U, Yerlikaya C, Turkan I, Bor M, Ozdemir F (2008) Response of the cherry rootstock to water stress induced invitro. Biol Plant 52(3):573

    Article  CAS  Google Scholar 

  • Sorkheh K, Shiran B, Rouhi V, Khodambashi M, Sofo A (2012) Salt stress induction of some key antioxidant enzymes and metabolites in eight Iranian wild almond species. Acta Physiol Plant 34(1):203–213

    Article  CAS  Google Scholar 

  • Sotiropoulos TE, Dimassi KN, Tsirakoglou V, Therios IN (2006) Responses of two Prunus rootstocks to KCl induced salinity invitro. Biol Plant 50(3):477–480

    Article  CAS  Google Scholar 

  • Tilkat EA, Kaplan A, Hoşer A, Tilkat E (2017) In vitro şartlarda yetiştirilen buttum (Pistacia khinjuk Stocks)’da çözünür karbonhidrat değerleri ile antioksidan peroksidaz aktivitesi üzerine tuz stresinin etkileri. Batman Üniversitesi Yaşam Bilimleri Dergisi 7(2/2):90–97

    Google Scholar 

  • Turfan N (2016) Yerel ceviz çeşidinde (Juglans regia L.) abiyotik stres faktörlerine karşı dayanıklılık mekanizmasının belirlenmesi. Anadolu Tarım Bilimleri Dergisi 31(3):321–331. https://doi.org/10.7161/omuanajas.269984

    Article  Google Scholar 

  • Valifard M, Mohsenzadeh S, Kholdebarin B, Rowshan V (2014) Effects of salt stress on volatile compounds, total phenolic content and antioxidant activities of Salvia mirzayanii. S Afr J Bot 93:92–97. https://doi.org/10.1016/j.sajb.2014.04.002

    Article  CAS  Google Scholar 

  • Yıldırım AN, Tekintaş E, Koyuncu F (2007) Isparta bölgesinde geç çiçeklenen ve üstün nitelikli meyve veren badem (Prunus amygdalus Batsch.) genotiplerinin seleksiyonu. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi 4(1–2):39–48

    Google Scholar 

  • Yıldız M, Terzi H, Cenkci S, Terzi E, Uruşak B (2010) Bitkilerde tuzluluğa toleransın fizyolojik ve biyokimyasal markörleri. Anadolu Univ Sci Technol Life Sci Biotechnol 1(1):33

    Google Scholar 

  • Yılmaz S, Temizgül R, Yürürdurmaz C, Kaplan M (2020) Oxidant and antioxidant enzyme response of redbine sweet sorghum under NaCl salinity stress. Bioagro 32(1):31–38

    Google Scholar 

  • Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64(4):555–559. https://doi.org/10.1016/S0308-8146(98)00102-2

    Article  CAS  Google Scholar 

  • Zhu Z, Wei G, Li J, Qian Q, Yu J (2004) Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.). Plant Sci 167(3):527–533. https://doi.org/10.1016/j.plantsci.2004.04.020

    Article  CAS  Google Scholar 

  • Zrig A, Tounekti T, Vadel AM, Mohamed HB, Valero D, Serrano M, Chaker C, Habib K (2011) Possible involvement of polyphenols and polyamines in salt tolerance of almond rootstocks. Plant Physiol Biochem 49(11):1313–1322. https://doi.org/10.1016/j.plaphy.2011.08.009

    Article  CAS  PubMed  Google Scholar 

  • Zrig A, Mohamed HB, Tounekti T, Khemira H, Serrano M, Valeroc D, Vadelet AM (2016) Effect of rootstock on salinity tolerance of sweet almond (cv. Mazzetto). S Afr J Bot 102:50–59. https://doi.org/10.1016/j.sajb.2015.09.001

    Article  CAS  Google Scholar 

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Acknowledgements

This research is an output of project number 215O779 supported by The Scientific and Technological Research Council of Turkey (TUBITAK).

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  1. Department of Horticultural, Faculty of Agriculture, Isparta University of Applied Science, Isparta, Turkey

    Adnan Nurhan Yildirim, Bekir Şan, Fatma Yildirim & Berna Bayar

  2. Department of Agricultural Biotechnology, Faculty of Agriculture, Isparta University of Applied Science, Isparta, Turkey

    Civan Çelik & Yaşar Karakurt

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  1. Adnan Nurhan Yildirim
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Correspondence to Adnan Nurhan Yildirim.

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A.N. Yildirim, B. Şan, F. Yildirim, C. Çelik, B. Bayar and Y. Karakurt declare that they have no competing interests.

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Yildirim, A.N., Şan, B., Yildirim, F. et al. Determining the Tolerance of Selected Almond Rootstock Genotypes to Salt Stress Under in vitro Conditions. Erwerbs-Obstbau 65, 299–310 (2023). https://doi.org/10.1007/s10341-022-00827-y

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