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Regeneration of plants from leaflet explants of tissue culture raised safed siris (Albizia procera)

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Abstract

Shoot bud regeneration was obtained from isolated leaflets of Albizia procera cultured on MS medium with various concentrations of 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). The highest numbers of adventitious buds were obtained on MS medium supplemented with 10 μM BA and 1 μM NAA. The replacement of 7 g l-1 Difco bacto agar with 2.6 g l-1 Phytagel in the medium enhanced adventitious bud regeneration. Further, addition of 15 μM silver nitrate promoted callus-free shoot regeneration from leaf explants. The regenerated shoot buds were elongated on MS medium containing 0.01 μM BA and 1 μM NAA. Rooting was obtained on modified MS medium supplemented with 2 μM IBA. To our knowledge this is the first report of direct regeneration of shoots from leaflet explants in A. procera, and should help facilitate genetic transformation in this species.

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Authors and Affiliations

  1. Genetics and Plant Propagation Division, Tropical Forest Research Institute, P.0. RFRC, Mandla Road, Jabalpur (M.P.), 482 021, India

    Sandeep Kumar & A.K. Sarkar

  2. Non Wood Forest Produce Division, Tropical Forest Research Institute, P.0. RFRC, Mandla Road, Jabalpur (M.P.), 482 021, India

    C. Kunhikannan

Authors
  1. Sandeep Kumar
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  2. A.K. Sarkar
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  3. C. Kunhikannan
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Kumar, S., Sarkar, A. & Kunhikannan, C. Regeneration of plants from leaflet explants of tissue culture raised safed siris (Albizia procera). Plant Cell, Tissue and Organ Culture 54, 137–143 (1998). https://doi.org/10.1023/A:1006179611034

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  • DOI: https://doi.org/10.1023/A:1006179611034

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