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Adventitious bud formation and shoot development from in vitro leaves of Vitis x Muscadinia hybrids

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Abstract

High levels of regeneration were obtained from young leaves excised from axillary shoots in proliferating nodal cultures of several Vitis x Muscadinia hybrids. Best results were obtained when the explants were cultured on solidified half-strength Murashige and Skoog medium supplemented with 8.9 μM 6-benzyladenine and 0.05 μM 1-naphthaleneacetic acid. Though variation was observed among the hybrids, the procedure used does not seem to be genotype-specific as all the hybrids and cultivars tested could regenerate. Scanning electron microscopy observations and histological studies carried out during the development of adventitious shoot organogenesis revealed that the promeristem initiation occurred in the outer cell layers near the wounded area of the petiolar stub.

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Abbreviations

BA:

6-benzyladenine

NAA:

1-naphthaleneacetic acid

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Authors and Affiliations

  1. Ecole Nationale Supérieure Agronomique, Place Pierre Viala, 34060, Montpellier Cedex, France

    L. Torregrosa

  2. Institut National de la Recherche Agronomique, Domaine du Chapître, 34751, Villeneuve lès Maguelone Cedex, France

    A. Bouquet

Authors
  1. L. Torregrosa
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  2. A. Bouquet
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Torregrosa, L., Bouquet, A. Adventitious bud formation and shoot development from in vitro leaves of Vitis x Muscadinia hybrids. Plant Cell Tiss Organ Cult 45, 245–252 (1996). https://doi.org/10.1007/BF00043637

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  • DOI: https://doi.org/10.1007/BF00043637

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